Overview

  • Product name

    Anti-IP10 antibody [EPR20764]
    See all IP10 primary antibodies
  • Description

    Rabbit monoclonal [EPR20764] to IP10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human IP10 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P02778

  • Positive control

    • WB: THP-1 cell treated with 200 ng/ml interferon-gamma (ab9659) and 50 ng/ml lipopolysaccharides (LPS) for 24 hours culture supernatant.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab214668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 12 kDa (predicted molecular weight: 10 kDa).

Target

  • Function

    Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications

    CXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Interferon gamma induced factor MOB1, mouse, homolog of antibody
    • Interferon gamma induced protein 10 antibody
    • 10 kDa interferon gamma induced protein antibody
    • 10 kDa interferon gamma-induced protein antibody
    • C X C motif chemokine 10 antibody
    • C7 antibody
    • Chemokine (C X C motif) ligand 10 antibody
    • Chemokine CXC motif ligand 10 antibody
    • Crg 2 antibody
    • CRG2 antibody
    • CXCL10 antibody
    • CXCL10(1-73) antibody
    • CXL10_HUMAN antibody
    • Gamma IP10 antibody
    • Gamma-IP10 antibody
    • gIP 10 antibody
    • GIP10 antibody
    • IFI10 antibody
    • INP 10 antibody
    • INP10 antibody
    • Interferon activated gene 10 antibody
    • Interferon activated gene 10 antibody
    • Interferon gamma induced cell line antibody
    • Interferon inducible cytokine IP 10 antibody
    • Interferon inducible cytokine IP10 antibody
    • IP 10 antibody
    • IP-10 antibody
    • Mob 1 antibody
    • MOB1 antibody
    • Protein 10 from interferon (gamma) induced cell line antibody
    • SCYB10 antibody
    • Small inducible cytokine B10 antibody
    • Small inducible cytokine B10 precursor antibody
    • Small inducible cytokine subfamily B (Cys X Cys) member 10 antibody
    • Small inducible cytokine subfamily B CXC member 10 antibody
    • Small inducible cytokine subfamily B, member 10 antibody
    • Small-inducible cytokine B10 antibody
    see all

Images

  • All lanes : Anti-IP10 antibody [EPR20764] (ab214668) at 1/1000 dilution

    Lane 1 : Untreated THP-1 (human monocytic leukemia cell line) culture supernatant
    Lane 2 : THP-1 treated with 200 ng/ml interferon-gamma (IFN-gamma, ab9659) and 50 ng/ml lipopolysaccharides (LPS) for 24 hours, culture supernatant

    Lysates/proteins at 15 µl per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 10 kDa
    Observed band size: 12 kDa
    why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    IP10 protein secretion can be induced by IFN-gamma treatment (PMID: 11907072).

References

ab214668 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab214668.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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