For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    ip10-antibody-epr20764-ab214668.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Innate Immunity Chemokines Alpha Chemokines (CXC)
Share by email
Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-IP10 antibody [EPR20764] (ab214668)

  • Datasheet
  • SDS
  • Certificate of Compliance
Submit a review Submit a question References (1)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-IP10 antibody [EPR20764] (ab214668)
  • Western blot - Anti-IP10 antibody [EPR20764] (ab214668)
  • Western blot - Anti-IP10 antibody [EPR20764] (ab214668)
  • ELISA - Anti-IP10 antibody [EPR20764] (ab214668)
  • Anti-IP10 antibody [EPR20764] (ab214668)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20764] to IP10
  • Suitable for: ELISA, WB
  • Knockout validated
  • Reacts with: Human, Recombinant fragment

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

ELISA
Product image
Human IP-10 ELISA Kit (ab173194)
Primary
Product image
Anti-CXCL11 antibody [EPR11076] (ab181035)
Knockout
Product image
Human CXCL10 (IP10) knockout A549 cell line (ab266971)

View more associated products

Overview

  • Product name

    Anti-IP10 antibody [EPR20764]
    See all IP10 primary antibodies
  • Description

    Rabbit monoclonal [EPR20764] to IP10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Human, Recombinant fragment
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild-type A549 IFN-y (ab259377) (100 ng/ml, 32 h) and TNF-alpha (ab259410) (10 ng/ml, 32h), and Brefeldin A (ab120299)-treated (5ug/ml for the last 6h) cell lysate; THP-1 IFN-y (ab259377) (200ng/ml, 24h) and LPS (50ng/ml, 24h)-treated for 24 hours, and Brefeldin A (ab120299)-treated (5ug/ml for the last 6h)
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20764
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Chemokines
    • Alpha Chemokines (CXC)
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic growth factors
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Cytokines and cytokine receptors ELISA kits
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Growth factors and hormones ELISA kits
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-IP10 antibody [EPR20764] - BSA and Azide free (ab224678)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human CXCL10 (IP10) knockout A549 cell line (ab266971)
    • Human CXCL10 (IP10) knockout THP-1 cell line (ab277860)
  • KO cell lysates

    • Human CXCL10 (IP10) knockout A549 cell lysate (ab256888)
  • Recombinant Protein

    • Recombinant human IP10 protein (ab9810)
  • Related Products

    • Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
    • Recombinant human Interferon gamma protein (Active) (ab9659)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab214668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
WB
1/1000. Detects a band of approximately 12 kDa (predicted molecular weight: 10 kDa).
Notes
ELISA
Use at an assay dependent concentration.
WB
1/1000. Detects a band of approximately 12 kDa (predicted molecular weight: 10 kDa).

Target

  • Function

    Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications

    CXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P02778 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3627 Human
    • Omim: 147310 Human
    • SwissProt: P02778 Human
    • Unigene: 632586 Human
    • Alternative names

      • Interferon gamma induced factor MOB1, mouse, homolog of antibody
      • Interferon gamma induced protein 10 antibody
      • 10 kDa interferon gamma induced protein antibody
      • 10 kDa interferon gamma-induced protein antibody
      • C X C motif chemokine 10 antibody
      • C7 antibody
      • Chemokine (C X C motif) ligand 10 antibody
      • Chemokine CXC motif ligand 10 antibody
      • Crg 2 antibody
      • CRG2 antibody
      • CXCL10 antibody
      • CXCL10(1-73) antibody
      • CXL10_HUMAN antibody
      • Gamma IP10 antibody
      • Gamma-IP10 antibody
      • gIP 10 antibody
      • GIP10 antibody
      • IFI10 antibody
      • INP 10 antibody
      • INP10 antibody
      • Interferon activated gene 10 antibody
      • Interferon activated gene 10 antibody
      • Interferon gamma induced cell line antibody
      • Interferon inducible cytokine IP 10 antibody
      • Interferon inducible cytokine IP10 antibody
      • IP 10 antibody
      • IP-10 antibody
      • Mob 1 antibody
      • MOB1 antibody
      • Protein 10 from interferon (gamma) induced cell line antibody
      • SCYB10 antibody
      • Small inducible cytokine B10 antibody
      • Small inducible cytokine B10 precursor antibody
      • Small inducible cytokine subfamily B (Cys X Cys) member 10 antibody
      • Small inducible cytokine subfamily B CXC member 10 antibody
      • Small inducible cytokine subfamily B, member 10 antibody
      • Small-inducible cytokine B10 antibody
      see all

    Images

    • Western blot - Anti-IP10 antibody [EPR20764] (ab214668)
      Western blot - Anti-IP10 antibody [EPR20764] (ab214668)
      All lanes : Anti-IP10 antibody [EPR20764] (ab214668) at 1/1000 dilution

      Lane 1 : Wild-type THP-1 vehicle control IFNg (0 ng/ml, 32 h), TNF-alpha (0 ng/ml, 32 h), Brefeldin A (5 ug/ml, 6 h) cell lysate
      Lane 2 : Wild-type THP-1 treated IFNg (100 ng/ml, 32 h), TNF-alpha (10 ng/ml, 32 h), Brefeldin A (5 ug/ml, 6 h) cell lysate
      Lane 3 : CXCL10 knockout THP-1 vehicle control IFNg (0 ng/ml, 32 h), TNF-alpha (0 ng/ml, 32 h), Brefeldin A (5 ug/ml, 6 h) cell lysate
      Lane 4 : CXCL10 knockout THP-1 treated IFN-gamma (100 ng/ml, 32 h), TNF-alpha (10 ng/ml, 32 h), Brefeldin A (5 ug/ml, 6 h) cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 10 kDa
      Observed band size: 11 kDa why is the actual band size different from the predicted?



      False colour image of Western blot: Anti-IP10 antibody [EPR20764] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab214668 was shown to bind specifically to IP10. A band was observed at 11 kDa in treated wild-type THP-1 cell lysates with no signal observed at this size in treated CXCL10 knockout cell line ab277860 (knockout cell lysate ab282997). To generate this image, wild-type and CXCL10 knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

    • Western blot - Anti-IP10 antibody [EPR20764] (ab214668)
      Western blot - Anti-IP10 antibody [EPR20764] (ab214668)
      All lanes : Anti-IP10 antibody [EPR20764] (ab214668) at 1/1000 dilution

      Lane 1 : Wild-type A549 Brefeldin A (ab120299)-treated (5ug/ml, 6h) cell lysate
      Lane 2 : Wild-type A549 IFN-y (ab259377) (100 ng/ml, 32 h) and TNF-alpha (ab259410) (10 ng/ml, 32h), and Brefeldin A (ab120299)-treated (5ug/ml for the last 6h) cell lysate
      Lane 3 : IP10 knockout A549 Brefeldin A (ab120299)-treated (5ug/ml, 6h) cell lysate
      Lane 4 : IP10 knockout A549 IFN-y (ab259377) (100ng/ml, 32h) and TNF-alpha (ab259410) (10ng/ml, 32h), and Brefeldin A (ab120299)-treated (5ug/ml for the last 6h) cell lysate
      Lane 5 : THP-1 Brefeldin A (ab120299)-treated (5ug/ml, 6h) cell lysate
      Lane 6 : THP-1 IFN-y (ab259377) (200ng/ml, 24h) and LPS (50ng/ml, 24h)-treated for 24 hours, and Brefeldin A (ab120299)-treated (5ug/ml for the last 6h) cell lysate

      Lysates/proteins at 30 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 10 kDa
      Observed band size: 11 kDa why is the actual band size different from the predicted?



      Lanes 1 - 6: Merged signal (red and green). Green - ab214668 observed at 11 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

      ab214668 was shown to react with IP10 in wild-type A549 cells in western blot with loss of signal observed in IP10 knockout cell line ab266971 (knockout cell lysate ab256888). Wild-type and IP10 knockout A549 cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab214668 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-IP10 antibody [EPR20764] (ab214668)
      Western blot - Anti-IP10 antibody [EPR20764] (ab214668)
      All lanes : Anti-IP10 antibody [EPR20764] (ab214668) at 1/1000 dilution

      Lane 1 : Untreated THP-1 (human monocytic leukemia cell line) culture supernatant
      Lane 2 : THP-1 treated with 200 ng/ml interferon-gamma (IFN-gamma, ab9659) and 50 ng/ml lipopolysaccharides (LPS) for 24 hours, culture supernatant

      Lysates/proteins at 15 µl per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Predicted band size: 10 kDa
      Observed band size: 12 kDa why is the actual band size different from the predicted?


      Exposure time: 15 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

      IP10 protein secretion can be induced by IFN-gamma treatment (PMID: 11907072).

    • ELISA - Anti-IP10 antibody [EPR20764] (ab214668)
      ELISA - Anti-IP10 antibody [EPR20764] (ab214668)
      ELISA analysis of CXCL10 recombinant protein at 1000 ng/mL with ab214668. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
    • Anti-IP10 antibody [EPR20764] (ab214668)
      Anti-IP10 antibody [EPR20764] (ab214668)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (1)

    Publishing research using ab214668? Please let us know so that we can cite the reference in this datasheet.

    ab214668 has been referenced in 1 publication.

    • Xu W  et al. The Role of CHK1 Varies with the Status of Oestrogen-receptor and Progesterone-receptor in the Targeted Therapy for Breast Cancer. Int J Biol Sci 16:1388-1402 (2020). PubMed: 32210727

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab214668.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2022 Abcam plc. All rights reserved.