Recombinant Anti-IP10 antibody [EPR20764] (ab214668)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20764] to IP10
- Suitable for: ELISA, WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-IP10 antibody [EPR20764]
See all IP10 primary antibodies -
Description
Rabbit monoclonal [EPR20764] to IP10 -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human IP10 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P02778 -
Positive control
- WB: THP-1 cell treated with 200 ng/ml interferon-gamma (ab9659) and 50 ng/ml lipopolysaccharides (LPS) for 24 hours culture supernatant; Wild-type A549 (IFN-y (100 ng/ml) plus TNF-alpha (10 ng/ml) 32 hours + Brefeldin A 5ug/ml the last 6h) cell lysate; THP-1 (IFN-y (200 ng/ml) plus LPS (50 ng/ml) 24 hours + Brefeldin A 5ug/ml the last 6h) cell lysate.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20764 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab214668 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. | |
| WB | 1/1000. Detects a band of approximately 12 kDa (predicted molecular weight: 10 kDa). |
Target
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Function
Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsCXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3627 Human
- Omim: 147310 Human
- SwissProt: P02778 Human
- Unigene: 632586 Human
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Alternative names
- Interferon gamma induced factor MOB1, mouse, homolog of antibody
- Interferon gamma induced protein 10 antibody
- 10 kDa interferon gamma induced protein antibody
see all
Images
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![Western blot - Anti-IP10 antibody [EPR20764] (ab214668)](https://www.abcam.com/ps/products/214/ab214668/Images/ab214668-413280-anti-ip10-antibody-epr20764-western-blot-wt-a549-untreated-control-32-hours-brefeldin-wt-a549-ifn-tnfalpha-brefeldin.jpg)
Western blot - Anti-IP10 antibody [EPR20764] (ab214668)All lanes : Anti-IP10 antibody [EPR20764] (ab214668) at 1/1000 dilution
Lane 1 : Wild-type A549 Brefeldin A-treated (5ug/ml, 6h)
Lane 2 : Wild-type A549 IFN-y (100 ng/ml) and TNF-alpha (10 ng/ml)-treated for 32 hours, and Brefeldin A (5ug/ml for the last 6h)
Lane 3 : IP10 knockout A549 Brefeldin A-treated (5ug/ml, 6h)
Lane 4 : IP10 knockout A549 IFN-y (100 ng/ml) and TNF-alpha (10 ng/ml)-treated for 32 hours, and Brefeldin A (5ug/ml for the last 6h)
Lane 5 : THP-1 Brefeldin A-treated (5ug/ml, 6h)
Lane 6 : THP-1 IFN-y (200 ng/ml) and LPS (50 ng/ml)-treated for 24 hours, and Brefeldin A (5ug/ml for the last 6h)
Lysates/proteins at 30 µg per lane.
Performed under reducing conditions.
Predicted band size: 10 kDa
Observed band size: 11 kDa why is the actual band size different from the predicted?Lanes 1 - 6: Merged signal (red and green). Green - ab214668 observed at 11 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab214668 was shown to react with IP10 in wild-type A549 cells in western blot with loss of signal observed in IP10 knockout cell line ab266971 (knockout cell lysate ab256888). Wild-type and IP10 knockout A549 cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab214668 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-IP10 antibody [EPR20764] (ab214668)](https://www.abcam.com/ps/products/214/ab214668/Images/ab214668-285819-anti-ip10-antibody-epr20764-western-blot.jpg)
Western blot - Anti-IP10 antibody [EPR20764] (ab214668)All lanes : Anti-IP10 antibody [EPR20764] (ab214668) at 1/1000 dilution
Lane 1 : Untreated THP-1 (human monocytic leukemia cell line) culture supernatant
Lane 2 : THP-1 treated with 200 ng/ml interferon-gamma (IFN-gamma, ab9659) and 50 ng/ml lipopolysaccharides (LPS) for 24 hours, culture supernatant
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 10 kDa
Observed band size: 12 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
IP10 protein secretion can be induced by IFN-gamma treatment (PMID: 11907072).
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![ELISA - Anti-IP10 antibody [EPR20764] (ab214668)](https://www.abcam.com/ps/products/214/ab214668/Images/ab214668-2-anti-ip10-antibody-epr20764-elisa-cxcl10.jpg)
ELISA - Anti-IP10 antibody [EPR20764] (ab214668)ELISA analysis of CXCL10 recombinant protein at 1000 ng/mL with ab214668. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab214668 has not yet been referenced specifically in any publications.
