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  1. Link

    ip10-antibody-epr24674-12-ab283681.pdf

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Immunology Innate Immunity Chemokines Alpha Chemokines (CXC)
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-IP10 antibody [EPR24674-12] (ab283681)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Western blot - Anti-IP10 antibody [EPR24674-12] (ab283681)
  • Immunocytochemistry/ Immunofluorescence - Anti-IP10 antibody [EPR24674-12] (ab283681)
  • Immunocytochemistry/ Immunofluorescence - Anti-IP10 antibody [EPR24674-12] (ab283681)
  • Anti-IP10 antibody [EPR24674-12] (ab283681)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24674-12] to IP10
  • Suitable for: ICC/IF, WB
  • Knockout validated
  • Reacts with: Human

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Overview

  • Product name

    Anti-IP10 antibody [EPR24674-12]
    See all IP10 primary antibodies
  • Description

    Rabbit monoclonal [EPR24674-12] to IP10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild-type A549 and THP-1 treated with IFN-y, LPS, and Brefeldin A whole cell lysates ICC/IF: THP-1 cells, and A549 cells treated with Interferon gamma, lipopolysaccharide, and Brefeldin A
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR24674-12
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Chemokines
    • Alpha Chemokines (CXC)
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic growth factors
    • Kits/ Lysates/ Other
    • Kits
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    • ELISA Kits
    • Cytokines and cytokine receptors ELISA kits
    • Kits/ Lysates/ Other
    • Kits
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    • Growth factors and hormones ELISA kits
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • KO cell lines

    • Human CXCL10 (IP10) knockout A549 cell line (ab266971)
  • KO cell lysates

    • Human CXCL10 (IP10) knockout A549 cell lysate (ab256888)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab283681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/50.
WB
1/1000. Predicted molecular weight: 10 kDa.
Notes
ICC/IF
1/50.
WB
1/1000. Predicted molecular weight: 10 kDa.
Application notes
Is unsuitable for IHC-P or IP.

Target

  • Function

    Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications

    CXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P02778 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3627 Human
    • Omim: 147310 Human
    • SwissProt: P02778 Human
    • Unigene: 632586 Human
    • Alternative names

      • Interferon gamma induced factor MOB1, mouse, homolog of antibody
      • Interferon gamma induced protein 10 antibody
      • 10 kDa interferon gamma induced protein antibody
      • 10 kDa interferon gamma-induced protein antibody
      • C X C motif chemokine 10 antibody
      • C7 antibody
      • Chemokine (C X C motif) ligand 10 antibody
      • Chemokine CXC motif ligand 10 antibody
      • Crg 2 antibody
      • CRG2 antibody
      • CXCL10 antibody
      • CXCL10(1-73) antibody
      • CXL10_HUMAN antibody
      • Gamma IP10 antibody
      • Gamma-IP10 antibody
      • gIP 10 antibody
      • GIP10 antibody
      • IFI10 antibody
      • INP 10 antibody
      • INP10 antibody
      • Interferon activated gene 10 antibody
      • Interferon activated gene 10 antibody
      • Interferon gamma induced cell line antibody
      • Interferon inducible cytokine IP 10 antibody
      • Interferon inducible cytokine IP10 antibody
      • IP 10 antibody
      • IP-10 antibody
      • Mob 1 antibody
      • MOB1 antibody
      • Protein 10 from interferon (gamma) induced cell line antibody
      • SCYB10 antibody
      • Small inducible cytokine B10 antibody
      • Small inducible cytokine B10 precursor antibody
      • Small inducible cytokine subfamily B (Cys X Cys) member 10 antibody
      • Small inducible cytokine subfamily B CXC member 10 antibody
      • Small inducible cytokine subfamily B, member 10 antibody
      • Small-inducible cytokine B10 antibody
      see all

    Images

    • Western blot - Anti-IP10 antibody [EPR24674-12] (ab283681)
      Western blot - Anti-IP10 antibody [EPR24674-12] (ab283681)
      All lanes : Anti-IP10 antibody [EPR24674-12] (ab283681) at 1/1000 dilution

      Lane 1 : Untreated Wild-type A549 (human lung carcinoma epithelial cell), whole cell lysate at 40 µg
      Lane 2 : Wild-type A549 treated with 100 ng/ml IFN-y (ab259377) for 32 hours and 10 ng/m TNF-alpha (ab259410) for 32 hours, and 5ug/ml Brefeldin A (ab120299) for the last 6 hours, whole cell lysate at 40 µg
      Lane 3 : Untreated IP10 knockout A549 whole cell lysate at 40 µg
      Lane 4 : IP10 knockout A549 treated with 100 ng/ml IFN-y (ab259377) for 32 hours and 10 ng/m TNF-alpha (ab259410) for 32 hours, and 5ug/ml Brefeldin A (ab120299) for the last 6 hours, whole cell lysate at 40 µg
      Lane 5 : Untreated THP-1 (human monocytic leukemia monocyte), whole cell lysate at 20 µg
      Lane 6 : THP-1 treated with 200ng/ml IFN-y (ab259377) for 24 hours and 50ng/ml LPS for 24 hours, and 5ug/ml Brefeldin A for the last 21 hours, whole cell lysate at 20 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (ab216773) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (ab216776) at 1/10000 dilution

      Predicted band size: 10 kDa
      Observed band size: 11 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      Lanes 1-6: Merged signal (red and green). Green - ab283681 observed at 11 kDa. Red-loading control ab8245 (Mouse monoclonal [6C5] to GAPDH) was observed at 36 kDa.

      ab283681 Anti-TNF Receptor I antibody [EPR24674-12] was shown to specifically react with IP10 in treated wild-type A549 cells. Loss of signal was observed when IP10 knockout cell lines ab266971 (knockout cell lysate ab256888) were used. Wild-type and IP10 knockout samples were subjected to SDS-PAGE. ab283681 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at 4°C overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

       

    • Immunocytochemistry/ Immunofluorescence - Anti-IP10 antibody [EPR24674-12] (ab283681)
      Immunocytochemistry/ Immunofluorescence - Anti-IP10 antibody [EPR24674-12] (ab283681)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 cells labelling IP10 with ab283681 at 1/50 (12.86 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in THP-1 cell line after treatment with Interferon gamma (200 ng/ml) and lipopolysaccharide (50 ng/ml) for 3 h, then adding Brefeldin A (1 ug/ml) for another 21 h. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-IP10 antibody [EPR24674-12] (ab283681)
      Immunocytochemistry/ Immunofluorescence - Anti-IP10 antibody [EPR24674-12] (ab283681)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized CXCL10 KO A549 (ab266971) cells labelling IP10 with ab283681 at 1/50 (12.86 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing the signal expression was increased in Parental A549 cells after treatment with Interferon gamma (200 ng/ml) and lipopolysaccharide (50 ng/ml) for 3h, then adding Brefeldin A (1 ug/ml) for another 21h, and no staining in treated CXCL10 KO A549 cells with the same conditions. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

    • Anti-IP10 antibody [EPR24674-12] (ab283681)
      Anti-IP10 antibody [EPR24674-12] (ab283681)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab283681? Please let us know so that we can cite the reference in this datasheet.

    ab283681 has not yet been referenced specifically in any publications.

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