Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-IP3 receptor antibody [EPR4537] (ab108517)

Overview

  • Product name

    Anti-IP3 receptor antibody [EPR4537]
    See all IP3 receptor primary antibodies
  • Description

    Rabbit monoclonal [EPR4537] to IP3 receptor
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human IP3 receptor aa 2200-2300. The exact sequence is proprietary.

  • Positive control

    • WB: Rat brain, SH-SY5Y, Mouse brain, and HeLa lysates IP: Mouse brain cells; IHC-P: Human and Mouse cerebellum tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab108517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 314 kDa.
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function

      Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate.
    • Tissue specificity

      Widely expressed.
    • Involvement in disease

      Defects in ITPR1 are the cause of spinocerebellar ataxia type 15 (SCA15) (SCA15) [MIM:606658]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA15 is an autosomal dominant cerebellar ataxia (ADCA). It is very slow progressing form with a wide range of onset, ranging from childhood to adult. Most patients remain ambulatory.
    • Sequence similarities

      Belongs to the InsP3 receptor family.
      Contains 5 MIR domains.
    • Domain

      The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region.
    • Post-translational
      modifications

      Phosphorylated by cAMP kinase. Phosphorylation prevents the ligand-induced opening of the calcium channels.
      Phosphorylated on tyrosine residues.
    • Cellular localization

      Endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • 4 antibody
      • 5-trisphosphate receptor antibody
      • 5-trisphosphate receptor type 1 antibody
      • DKFZp313E1334 antibody
      • DKFZp313N1434 antibody
      • inositol 1 4 5 triphosphate receptor type 1 antibody
      • Inositol 1 4 5 trisphosphate Receptor Type 1 antibody
      • Inositol 1 antibody
      • InsP3R1 antibody
      • IP3 antibody
      • IP3 receptor antibody
      • IP3 receptor isoform 1 antibody
      • IP3R 1 antibody
      • IP3R antibody
      • IP3R1 antibody
      • ITPR 1 antibody
      • Itpr1 antibody
      • ITPR1_HUMAN antibody
      • SCA15 antibody
      • SCA16 antibody
      • SCA29 antibody
      • Type 1 inositol 1 4 5 trisphosphate receptor antibody
      • Type 1 inositol 1 antibody
      • Type 1 InsP3 receptor antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: IP3 receptor knockout HAP1 cell lysate (20 µg)
      Lane 3: Human brain tissue lysate (20 µg)
      Lane 4: SH-SY5Y cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab108517 observed at 270 kDa. Red - loading control, ab18058, observed at 124 kDa.


      ab108517 was shown to specifically recognize IP3 receptor in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when IP3 receptor knockout samples were examined. Wild-type and IP3 receptor knockout samples were subjected to SDS-PAGE. ab108517 and ab18058 (loading control to Vinculin) were diluted 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemical analysis of paraffin-embedded Human cerebellum  tissue labeling IP3 with ab108517 at 1/1000 dilution (0.139 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebellum. The section was incubated with ab108517 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    • Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling IP3 with ab108517 at 1/1000 dilution (0.139 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebellum. The section was incubated with ab108517 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    • ab108517 (purified) at 1:500 dilution (1.124 µg/ml) immunoprecipitating IP3 receptor in Mouse brain lysate.
      Lane 1 (input): Mouse brain lysate 10µg
      Lane 2 (+): ab108517 & Mouse brain lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32061 in HeLa whole cell lysate
      For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM /TBST .
    • All lanes : Anti-IP3 receptor antibody [EPR4537] (ab108517) at 1/1000 dilution

      Lane 1 : Rat brain lysate
      Lane 2 : SH-SY5Y lysate
      Lane 3 : Mouse brain lysate
      Lane 4 : HeLa brain lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 314 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab108517 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Question
    Answer

    Please find here below the promised information about the ab108517 as well. I know it might not be useful to you anymore, but I received now the answer from the laboratory. As already mentioned, we do not have tested this antibody against bovine proteins. Please find here however the results of the alignments of the immunogen of ab108517 with the different bovine sequences: ITPR1 - 100% aas homology ITPR2 - ~90% aas homology ITPR3 - ~90% aas homology This information is surely good to keep in mind, if the other antibody you are currently testing will not crossreact to the bovine protein. I wish you good success with your research and am looking forward to hear back from you.

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