For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    ip3-receptor-antibody-l2418-ab252536.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Signaling Pathway Calcium Signaling Other
Share by email
Recombinant

Recombinant Anti-IP3 receptor antibody [L24/18] (ab252536)

  • Datasheet
  • SDS
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Flow Cytometry - Anti-IP3 receptor antibody [L24/18] (ab252536)
  • Western blot - Anti-IP3 receptor antibody [L24/18] (ab252536)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)
  • Immunohistochemistry (Frozen sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)
  • Immunohistochemistry (Frozen sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)
  • Anti-IP3 receptor antibody [L24/18] (ab252536)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [L24/18] to IP3 receptor
  • Suitable for: IHC-Fr, WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

View more associated products

Overview

  • Product name

    Anti-IP3 receptor antibody [L24/18]
    See all IP3 receptor primary antibodies
  • Description

    Mouse monoclonal [L24/18] to IP3 receptor
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-Fr
    Mouse
    Rat
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa and HuT-78 whole cell lysates; mouse brain lysate; rat brain lysate. IHC-P: Rat cerebellum tissue; mouse cerebellum tissue; human cerebellum tissue. IHC-Fr: Mouse cerebellum tissue; rat cerebellum tissue. Flow cyt: SH-SY5Y cells.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    L24/18
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Other
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia

Associated products

  • Alternative Versions

    • Anti-IP3 receptor antibody [L24/18] - BSA and Azide free (ab255762)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab252536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
IHC-Fr
Mouse
Rat
IHC-P
Mouse
Rat
Human
WB
Mouse
Rat
Human
Application Abreviews Notes
IHC-Fr
Use a concentration of 4.33 µg/ml.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20.

WB
Use a concentration of 0.433 µg/ml. Detects a band of approximately 314 kDa (predicted molecular weight: 314 kDa).
IHC-P
Use a concentration of 0.108 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt
Use a concentration of 10.825 µg/ml.
Notes
IHC-Fr
Use a concentration of 4.33 µg/ml.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20.

WB
Use a concentration of 0.433 µg/ml. Detects a band of approximately 314 kDa (predicted molecular weight: 314 kDa).
IHC-P
Use a concentration of 0.108 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt
Use a concentration of 10.825 µg/ml.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function

      Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate.
    • Tissue specificity

      Widely expressed.
    • Involvement in disease

      Defects in ITPR1 are the cause of spinocerebellar ataxia type 15 (SCA15) (SCA15) [MIM:606658]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA15 is an autosomal dominant cerebellar ataxia (ADCA). It is very slow progressing form with a wide range of onset, ranging from childhood to adult. Most patients remain ambulatory.
    • Sequence similarities

      Belongs to the InsP3 receptor family.
      Contains 5 MIR domains.
    • Domain

      The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region.
    • Post-translational
      modifications

      Phosphorylated by cAMP kinase. Phosphorylation prevents the ligand-induced opening of the calcium channels.
      Phosphorylated on tyrosine residues.
    • Cellular localization

      Endoplasmic reticulum membrane.
    • Target information above from: UniProt accession Q14643 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3708 Human
      • Entrez Gene: 16438 Mouse
      • Entrez Gene: 25262 Rat
      • Omim: 147265 Human
      • SwissProt: Q14643 Human
      • SwissProt: P11881 Mouse
      • SwissProt: P29994 Rat
      • Unigene: 567295 Human
      • Unigene: 715765 Human
      • Unigene: 227912 Mouse
      • Unigene: 2135 Rat
      see all
    • Alternative names

      • 4 antibody
      • 5-trisphosphate receptor antibody
      • 5-trisphosphate receptor type 1 antibody
      • DKFZp313E1334 antibody
      • DKFZp313N1434 antibody
      • inositol 1 4 5 triphosphate receptor type 1 antibody
      • Inositol 1 4 5 trisphosphate Receptor Type 1 antibody
      • Inositol 1 antibody
      • InsP3R1 antibody
      • IP3 antibody
      • IP3 receptor antibody
      • IP3 receptor isoform 1 antibody
      • IP3R 1 antibody
      • IP3R antibody
      • IP3R1 antibody
      • ITPR 1 antibody
      • Itpr1 antibody
      • ITPR1_HUMAN antibody
      • SCA15 antibody
      • SCA16 antibody
      • SCA29 antibody
      • Type 1 inositol 1 4 5 trisphosphate receptor antibody
      • Type 1 inositol 1 antibody
      • Type 1 InsP3 receptor antibody
      see all

    Images

    • Flow Cytometry - Anti-IP3 receptor antibody [L24/18] (ab252536)
      Flow Cytometry - Anti-IP3 receptor antibody [L24/18] (ab252536)

      Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized SH-SY5Y cells labeling IP3 receptor with ab252536 at 10.825µg/ml (red) compared with a Isotype control details (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti mouse IgG (Alexa Fluor® 488, ab150113), at 1/2000 dilution was used as the secondary antibody.

    • Western blot - Anti-IP3 receptor antibody [L24/18] (ab252536)
      Western blot - Anti-IP3 receptor antibody [L24/18] (ab252536)
      All lanes : Anti-IP3 receptor antibody [L24/18] (ab252536) at 1/1000 dilution

      Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
      Lane 2 : HuT-78 (human Sezary syndrome cutaneous T lymphocyte), whole cell lysate
      Lane 3 : Mouse brain tissue lysate
      Lane 4 : Rat brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG at 1/10000 dilution

      Predicted band size: 314 kDa
      Observed band size: 314 kDa



      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3.25 seconds.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)

      Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling IP3 receptor with ab252536 at 0.108µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Positive staining on rat cerebellum is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

      The section was incubated with ab252536 for 30 mins at RT.

      The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)

      Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling IP3 receptor with ab252536 at 0.108µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Positive staining on human cerebellum is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

      The section was incubated with ab252536 for 30 mins at RT.

      The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)

      Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling IP3 receptor with ab252536 at 0.108µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Positive staining on mouse cerebellum is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

      The section was incubated with ab252536 for 30 mins at RT.

      The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    • Immunohistochemistry (Frozen sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)
      Immunohistochemistry (Frozen sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)

      Immunohistochemical analysis of frozen section of 4%PFA-fixed, 0.2% Triton X-100 permeabilized mouse cerebellum tissue labeling IP3 receptor with ab252536 at 4.33µg/ml, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (green). Positive staining on mouse cerebellum is observed. The nuclear counter stain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody isb150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20.

       

    • Immunohistochemistry (Frozen sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)
      Immunohistochemistry (Frozen sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)

      Immunohistochemical analysis of frozen section of 4%PFA-fixed, 0.2% Triton X-100 permeabilized rat cerebellum tissue labeling IP3 receptor with ab252536 at 4.33µg/ml, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (green). Positive staining on rat cerebellum is observed. The nuclear counter stain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody isb150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20.

    • Anti-IP3 receptor antibody [L24/18] (ab252536)
      Anti-IP3 receptor antibody [L24/18] (ab252536)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab252536? Please let us know so that we can cite the reference in this datasheet.

    ab252536 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab252536.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.