Recombinant

Recombinant Anti-IP3 receptor antibody [L24/18] (ab252536)

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Overview

  • Product name

    Anti-IP3 receptor antibody [L24/18]
    See all IP3 receptor primary antibodies

  • Description

    Mouse monoclonal [L24/18] to IP3 receptor

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-Fr, WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is considered to be commercially sensitive.

  • Positive control

    • WB: HeLa and HuT-78 whole cell lysates; mouse brain lysate; rat brain lysate. IHC-P: Rat cerebellum tissue; mouse cerebellum tissue; human cerebellum tissue. IHC-Fr: Mouse cerebellum tissue; rat cerebellum tissue. Flow cyt: SH-SY5Y cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Applications

Our Abpromise guarantee covers the use of ab252536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20.
WB 1/1000. Detects a band of approximately 314 kDa (predicted molecular weight: 314 kDa).
IHC-P 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt 1/40.
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    • Function

      Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate.

    • Tissue specificity

      Widely expressed.

    • Involvement in disease

      Defects in ITPR1 are the cause of spinocerebellar ataxia type 15 (SCA15) (SCA15) [MIM:606658]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA15 is an autosomal dominant cerebellar ataxia (ADCA). It is very slow progressing form with a wide range of onset, ranging from childhood to adult. Most patients remain ambulatory.

    • Sequence similarities

      Belongs to the InsP3 receptor family.
      Contains 5 MIR domains.

    • Domain

      The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region.

    • Post-translational
      modifications

      Phosphorylated by cAMP kinase. Phosphorylation prevents the ligand-induced opening of the calcium channels.
      Phosphorylated on tyrosine residues.

    • Cellular localization

      Endoplasmic reticulum membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • 4 antibody
      • 5-trisphosphate receptor antibody
      • 5-trisphosphate receptor type 1 antibody
      • DKFZp313E1334 antibody
      • DKFZp313N1434 antibody
      • inositol 1 4 5 triphosphate receptor type 1 antibody
      • Inositol 1 4 5 trisphosphate Receptor Type 1 antibody
      • Inositol 1 antibody
      • InsP3R1 antibody
      • IP3 antibody
      • IP3 receptor antibody
      • IP3 receptor isoform 1 antibody
      • IP3R 1 antibody
      • IP3R antibody
      • IP3R1 antibody
      • ITPR 1 antibody
      • Itpr1 antibody
      • ITPR1_HUMAN antibody
      • SCA15 antibody
      • SCA16 antibody
      • SCA29 antibody
      • Type 1 inositol 1 4 5 trisphosphate receptor antibody
      • Type 1 inositol 1 antibody
      • Type 1 InsP3 receptor antibody
      see all

    Images

    • Flow Cytometry - Anti-IP3 receptor antibody [L24/18] (ab252536)
      Flow Cytometry - Anti-IP3 receptor antibody [L24/18] (ab252536)

      Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized SH-SY5Y cells labeling IP3 receptor with ab252536 at 1/40 dilution (red) compared with a Isotype control details (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti mouse IgG (Alexa Fluor® 488, ab150113), at 1/2000 dilution was used as the secondary antibody.

    • Western blot - Anti-IP3 receptor antibody [L24/18] (ab252536)
      Western blot - Anti-IP3 receptor antibody [L24/18] (ab252536)
      All lanes : Anti-IP3 receptor antibody [L24/18] (ab252536) at 1/1000 dilution

      Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
      Lane 2 : HuT-78 (human Sezary syndrome cutaneous T lymphocyte), whole cell lysate
      Lane 3 : Mouse brain tissue lysate
      Lane 4 : Rat brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG at 1/10000 dilution

      Predicted band size: 314 kDa
      Observed band size: 314 kDa



      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3.25 seconds.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)

      Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling IP3 receptor with ab252536 at 1/4000 dilution, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Positive staining on rat cerebellum is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

      The section was incubated with ab252536 for 30 mins at RT.

      The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)

      Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling IP3 receptor with ab252536 at 1/4000 dilution, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Positive staining on human cerebellum is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

      The section was incubated with ab252536 for 30 mins at RT.

      The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)

      Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling IP3 receptor with ab252536 at 1/4000 dilution, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Positive staining on mouse cerebellum is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

      The section was incubated with ab252536 for 30 mins at RT.

      The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    • Immunohistochemistry (Frozen sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)
      Immunohistochemistry (Frozen sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)

      Immunohistochemical analysis of frozen section of 4%PFA-fixed, 0.2% Triton X-100 permeabilized mouse cerebellum tissue labeling IP3 receptor with ab252536 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (green). Positive staining on mouse cerebellum is observed. The nuclear counter stain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody isb150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20.

       

    • Immunohistochemistry (Frozen sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)
      Immunohistochemistry (Frozen sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)

      Immunohistochemical analysis of frozen section of 4%PFA-fixed, 0.2% Triton X-100 permeabilized rat cerebellum tissue labeling IP3 receptor with ab252536 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (green). Positive staining on rat cerebellum is observed. The nuclear counter stain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody isb150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20.

    References

    ab252536 has not yet been referenced specifically in any publications.

    Publishing research using ab252536? Please let us know so that we can cite the reference in this datasheet.

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