Recombinant Anti-IP3 receptor antibody [L24/18] (ab252536)
![Flow Cytometry - Anti-IP3 receptor antibody [L24/18] (ab252536)](https://www.abcam.com/ps/products/252/ab252536/Images/ab252536-365731-anti-ip3-receptor-antibody-flow-cytometry-human.jpg "Flow Cytometry - Anti-IP3 receptor antibody [L24/18] (ab252536)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [L24/18] to IP3 receptor
- Suitable for: IHC-Fr, WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-IP3 receptor antibody [L24/18]
See all IP3 receptor primary antibodies -
Description
Mouse monoclonal [L24/18] to IP3 receptor -
Host species
Mouse -
Tested applications
Suitable for: IHC-Fr, WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa and HuT-78 whole cell lysates; mouse brain lysate; rat brain lysate. IHC-P: Rat cerebellum tissue; mouse cerebellum tissue; human cerebellum tissue. IHC-Fr: Mouse cerebellum tissue; rat cerebellum tissue. Flow cyt: SH-SY5Y cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
L24/18 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab252536 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
Use a concentration of 4.33 µg/ml.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20. |
|
| WB |
Use a concentration of 0.433 µg/ml. Detects a band of approximately 314 kDa (predicted molecular weight: 314 kDa).
|
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| IHC-P |
Use a concentration of 0.108 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| Flow Cyt |
Use a concentration of 10.825 µg/ml.
|
| Notes |
|---|
|
IHC-Fr
Use a concentration of 4.33 µg/ml. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20. |
|
WB
Use a concentration of 0.433 µg/ml. Detects a band of approximately 314 kDa (predicted molecular weight: 314 kDa). |
|
IHC-P
Use a concentration of 0.108 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
Flow Cyt
Use a concentration of 10.825 µg/ml. |
Target
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Function
Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate. -
Tissue specificity
Widely expressed. -
Involvement in disease
Defects in ITPR1 are the cause of spinocerebellar ataxia type 15 (SCA15) (SCA15) [MIM:606658]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA15 is an autosomal dominant cerebellar ataxia (ADCA). It is very slow progressing form with a wide range of onset, ranging from childhood to adult. Most patients remain ambulatory. -
Sequence similarities
Belongs to the InsP3 receptor family.
Contains 5 MIR domains. -
Domain
The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region. -
Post-translational
modificationsPhosphorylated by cAMP kinase. Phosphorylation prevents the ligand-induced opening of the calcium channels.
Phosphorylated on tyrosine residues. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 3708 Human
- Entrez Gene: 16438 Mouse
- Entrez Gene: 25262 Rat
- Omim: 147265 Human
- SwissProt: Q14643 Human
- SwissProt: P11881 Mouse
- SwissProt: P29994 Rat
- Unigene: 567295 Human
see all -
Alternative names
- 4 antibody
- 5-trisphosphate receptor antibody
- 5-trisphosphate receptor type 1 antibody
see all
Images
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Flow Cytometry - Anti-IP3 receptor antibody [L24/18] (ab252536)
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized SH-SY5Y cells labeling IP3 receptor with ab252536 at 10.825µg/ml (red) compared with a Isotype control details (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti mouse IgG (Alexa Fluor® 488, ab150113), at 1/2000 dilution was used as the secondary antibody.
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![Western blot - Anti-IP3 receptor antibody [L24/18] (ab252536)](https://www.abcam.com/ps/products/252/ab252536/Images/ab252536-365724-anti-ip3-receptor-antibody-western-blot.jpg)
Western blot - Anti-IP3 receptor antibody [L24/18] (ab252536)All lanes : Anti-IP3 receptor antibody [L24/18] (ab252536) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : HuT-78 (human Sezary syndrome cutaneous T lymphocyte), whole cell lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG at 1/10000 dilution
Predicted band size: 314 kDa
Observed band size: 314 kDaBlocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3.25 seconds.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)](https://www.abcam.com/ps/products/252/ab252536/Images/ab252536-365727-anti-ip3-receptor-antibody-immunohistochemistry-cerebellum-rat.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling IP3 receptor with ab252536 at 0.108µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Positive staining on rat cerebellum is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with ab252536 for 30 mins at RT.
The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)](https://www.abcam.com/ps/products/252/ab252536/Images/ab252536-365725-anti-ip3-receptor-antibody-immunohistochemistry-cerebellum-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling IP3 receptor with ab252536 at 0.108µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Positive staining on human cerebellum is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with ab252536 for 30 mins at RT.
The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)](https://www.abcam.com/ps/products/252/ab252536/Images/ab252536-365726-anti-ip3-receptor-antibody-immunohistochemistry-cerebellum-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling IP3 receptor with ab252536 at 0.108µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Positive staining on mouse cerebellum is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with ab252536 for 30 mins at RT.
The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
-
![Immunohistochemistry (Frozen sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)](https://www.abcam.com/ps/products/252/ab252536/Images/ab252536-365728-anti-ip3-receptor-antibody-immunohistochemistry-frozen-cerebellum-mouse.jpg)
Immunohistochemistry (Frozen sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)Immunohistochemical analysis of frozen section of 4%PFA-fixed, 0.2% Triton X-100 permeabilized mouse cerebellum tissue labeling IP3 receptor with ab252536 at 4.33µg/ml, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (green). Positive staining on mouse cerebellum is observed. The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody isb150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20.
-
![Immunohistochemistry (Frozen sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)](https://www.abcam.com/ps/products/252/ab252536/Images/ab252536-365729-anti-ip3-receptor-antibody-immunohistochemistry-frozen-cerebellum-rat.jpg)
Immunohistochemistry (Frozen sections) - Anti-IP3 receptor antibody [L24/18] (ab252536)Immunohistochemical analysis of frozen section of 4%PFA-fixed, 0.2% Triton X-100 permeabilized rat cerebellum tissue labeling IP3 receptor with ab252536 at 4.33µg/ml, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (green). Positive staining on rat cerebellum is observed. The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody isb150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20.
-
![Anti-IP3 receptor antibody [L24/18] (ab252536)](https://www.abcam.com/ps/products/252/ab252536/Images/ab252536-8-benefits-of-recombinant-antibodies.png)
Anti-IP3 receptor antibody [L24/18] (ab252536)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab252536 has not yet been referenced specifically in any publications.
