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    ip3r1-antibody-ab264280.pdf

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Neuroscience Neurotransmission Calcium Signaling IP3R
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Anti-IP3R1 antibody (ab264280)

  • Datasheet
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Abpromise

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Western blot - Anti-IP3R1 antibody (ab264280)
  • Immunoprecipitation - Anti-IP3R1 antibody (ab264280)

Key features and details

  • Rabbit polyclonal to IP3R1
  • Suitable for: IP, WB
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-IP3R1 antibody
    See all IP3R1 primary antibodies
  • Description

    Rabbit polyclonal to IP3R1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human IP3R1 aa 1025-1050. The exact sequence is proprietary.
    Database link: Q14643

  • Positive control

    • WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Calcium Signaling
    • IP3R

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab264280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IP
Human
WB
Human
All applications
Mouse
Rat
Cow
Application Abreviews Notes
IP
Use at 2-5 µg/mg of lysate.
WB
1/2000 - 1/10000. Predicted molecular weight: 314 kDa.
Notes
IP
Use at 2-5 µg/mg of lysate.
WB
1/2000 - 1/10000. Predicted molecular weight: 314 kDa.

Target

  • Database links

    • Entrez Gene: 317697 Cow
    • Entrez Gene: 3708 Human
    • Entrez Gene: 16438 Mouse
    • Entrez Gene: 25262 Rat
    • Omim: 147265 Human
    • SwissProt: Q9TU34 Cow
    • SwissProt: Q14643 Human
    • SwissProt: P11881 Mouse
    • SwissProt: P29994 Rat
    • Unigene: 149900 Human
    see all
  • Alternative names

    • Inositol 1 4 5 Triphosphate Receptor 1 antibody
    • Inositol 1 4 5 trisphosphate receptor type 1 antibody
    • InsP3R1 antibody
    • IP3 receptor isoform 1 antibody
    • IP3R 1 antibody
    • IP3R antibody
    • ITPR 1 antibody
    • ITPR1 antibody
    • Type 1 inositol 1 4 5 trisphosphate receptor antibody
    • Type 1 InsP3 receptor antibody
    see all

Images

  • Western blot - Anti-IP3R1 antibody (ab264280)
    Western blot - Anti-IP3R1 antibody (ab264280)
    All lanes : Anti-IP3R1 antibody (ab264280) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 314 kDa


    Exposure time: 3 minutes


    3-8% SDS-PAGE

  • Immunoprecipitation - Anti-IP3R1 antibody (ab264280)
    Immunoprecipitation - Anti-IP3R1 antibody (ab264280)

    IP3R1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264280 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264280 at 0.4 µg/ml.

    Lane 1: ab264280 IP in HeLa whole cell lysate.
    Lane 2: Control IgG.

    Exposure time: 15 secs.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab264280? Please let us know so that we can cite the reference in this datasheet.

    ab264280 has not yet been referenced specifically in any publications.

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