Product nameAnti-IP3R1 antibody
See all IP3R1 primary antibodies
DescriptionRabbit polyclonal to IP3R1
Tested applicationsSuitable for: IP, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic peptide within Human IP3R1 aa 2708-2758. The exact sequence is proprietary.
Database link: Q14643
- IHC-P: Mouse cerebellum tissue. WB: HeLa whole cell lysate. IP: HeLa whole cell lysate. IF: Mouse cerebellum tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 314 kDa.|
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse cerebellum tissue labeling IP3R1 with ab264281 at 1/100 dilution. Detection: Red fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1/100. Counterstain: DAPI (blue).
All lanes : Anti-IP3R1 antibody (ab264281) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 314 kDa
Exposure time: 30 seconds
IP3R1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264281 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264281 at 0.4 µg/ml.
Lane 1: ab264281 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 30 secs.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse cerebellum tissue labeling IP3R1 with ab264281 at 1/200 dilution. Detection: DAB. Counterstain: IHC Hematoxylin (blue).
ab264281 has not yet been referenced specifically in any publications.