Product nameAnti-IP3R1 antibody
See all IP3R1 primary antibodies
DescriptionRabbit polyclonal to IP3R1
Tested applicationsSuitable for: IP, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic peptide within Human IP3R1 aa 2708-2758. The exact sequence is proprietary.
Database link: Q14643
- IHC-P: Mouse cerebellum tissue. WB: HeLa whole cell lysate. IP: HeLa whole cell lysate. IF: Mouse cerebellum tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 314 kDa.|
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- InsP3R1 antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse cerebellum tissue labeling IP3R1 with ab264281 at 1/100 dilution. Detection: Red fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1/100. Counterstain: DAPI (blue).
All lanes : Anti-IP3R1 antibody (ab264281) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 314 kDa
Exposure time: 30 seconds
IP3R1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264281 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264281 at 0.4 µg/ml.
Lane 1: ab264281 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 30 secs.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse cerebellum tissue labeling IP3R1 with ab264281 at 1/200 dilution. Detection: DAB. Counterstain: IHC Hematoxylin (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264281 has not yet been referenced specifically in any publications.