Recombinant
RabMAb

Recombinant Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] - BSA and Azide free (ab238170)

Overview

  • Product name

    Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] - BSA and Azide free
    See all IP6K1 primary antibodies
  • Description

    Rabbit monoclonal [EPR19852] to IP6K1 (phospho S118 + S121) - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Dot blot, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human IP6K1 aa 100-200.
    Database link: Q92551

  • Positive control

    • WB: HEK-293T transfected with a His-tagged human IP6K1 (WT) expression vector, whole cell lysate.
  • General notes

    Ab238170 is the carrier-free version of ab214816. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab238170 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab238170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa).
Dot blot Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Function

    Converts inositol hexakisphosphate (InsP6) to diphosphoinositol pentakisphosphate (InsP7/PP-InsP5). Converts 1,3,4,5,6-pentakisphosphate (InsP5) to PP-InsP4.
  • Sequence similarities

    Belongs to the inositol phosphokinase (IPK) family.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATP:1D myo inositol hexakisphosphate phosphotransferase antibody
    • IHPK1 antibody
    • Inositol hexakisphosphate kinase 1 antibody
    • Inositol hexaphosphate kinase 1 antibody
    • InsP6 antibody
    • InsP6 kinase 1 antibody
    • Ip6k1 antibody
    • IP6K1_HUMAN antibody
    • Itpk6 antibody
    • Pi uptake stimulator antibody
    • PiUS antibody
    see all

Images

  • All lanes : Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (ab214816) at 1/10000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a His-tagged human IP6K1 (WT) expression vector, whole cell lysate
    Lane 2 : HEK-293T transfected with a His-tagged human IP6K1 (WT) expression vector, whole cell lysate. The WB membrane was treated with alkaline phosphatase for 1h at 37°C
    Lane 3 : HEK-293T transfected with a His-tagged human IP6K1 S118A+S121A mutant expression vector, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 50 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?


    Exposure time: 26 seconds


    Blocking/diluting buffer: 5% NFDM/TBST.

    The band at approximately 30 kDa likely represents degradation fragments of IP6K1 (pS118 and pS121) protein.

    The plasmids were kindly provided by our collaborator Dr. Feng Rao NIBS.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214816).

  • IP6K1 (phospho S118 + S121) was immunoprecipitated from 0.35 mg MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate with ab214816 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab214816 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.


    Lane 1: MOLT-4 whole cell lysate 10 µg (Input).
    Lane 2: ab214816 IP in MOLT-4 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab214816 in MOLT-4 whole cell lysate (-).

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 minute.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214816).

  • Dot blot analysis of IP6K1 (phospho S118 + S121) labeled with ab214816 at 1/1000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody was used at 1/100,000 dilution..

    Lane 1: IP6K1 (phospho S118 + S121) peptide (aa114-125).

    Lane 2: IP6K1 (phospho S118 + S121) peptide (aa116-127).

    Lane 3: IP6K1 (phospho S118) peptide (aa114-127).

    Lane 4: IP6K1 (phospho S121) peptide (aa114-127).

    Lane 5: IP6K1 non-phospho peptide (aa114-127).

    Exposure time: 58 seconds.

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214816).

References

ab238170 has not yet been referenced specifically in any publications.

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