Overview

  • Product name

    Ipilimumab ELISA Kit (Yervoy®)
    See all Ipilimumab kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 15%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 15%
  • Sample type

    Serum, Plasma
  • Assay type

    Quantitative
  • Sensitivity

    10 ng/ml
  • Range

    10 ng/ml - 1000 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 85% - 115%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Ipilimumab ELISA Kit (Yervoy®) (ab237640) is a highly specific and sensitive kit designed for the in vitro determination of Ipilimumab in human serum and plasma. The density of color is proportional to the amount of Ipilimumab captured from the samples and can be quantified when compared with standard curve.


    Ipilimumab (Yervoy®) is a fully human IgG1κ antibody that binds to CTLA-4 (cytotoxic T lymphocyte associated antigen 4), a molecule on Tcells that is indicated for unresectable or metastatic melanoma. The absence or presence of CTLA-4 can augment or suppress the immune system's T-cell response in fighting disease. Ipilimumab is designed to block the activity of CTLA-4, thereby sustaining an active immune response in its attack on cancer cells. The proposed mechanism of action is indirect, and may be through T-cell - mediated anti-tumor immune responses.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 96 tests
    Assay Buffer 1 x 50ml
    HRP-conjugate Probe 1 x 12ml
    Ipilimumab Standard S1 1 x 0.3ml
    Ipilimumab Standard S2 1 x 0.3ml
    Ipilimumab Standard S3 1 x 0.3ml
    Ipilimumab Standard S4 1 x 0.3ml
    Ipilimumab Standard S5 1 x 0.3ml
    Ipilimumab Standard S6 1 x 0.3ml
    Ipilimumab Standard S7 1 x 0.3ml
    Micro ELISA Plate 1 unit
    Plate sealers 2 units
    Stop Solution 1 x 12ml
    TMB substrate  1 x 12ml
    Wash buffer (20X) 1 x 50ml
  • Relevance

    Ipilimumab (Yervoy®) is a fully human IgG1? antibody that binds to CTLA-4 (cytotoxic T lymphocyteassociated antigen 4), a molecule on Tcells that is indicated for unresectable or metastatic melanoma. The absence or presence of CTLA-4 can augment or suppress the immune system's T-cell response in fighting disease. Ipilimumab is designed to block the activity of CTLA-4, thereby sustaining an active immune response in its attack on cancer cells. The proposed mechanism of action is indirect, and may be through T-cell - mediated anti-tumor immune responses.
  • Alternative names

    • Yervoy

Applications

Our Abpromise guarantee covers the use of ab237653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Typical Standard Curve: This standard curve is for demonstration only. A standard curve must be run with each assay.

Protocols

References

ab237653 has not yet been referenced specifically in any publications.

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