Key features and details
- Rabbit polyclonal to IPO11
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IPO11 antibody
See all IPO11 primary antibodies
DescriptionRabbit polyclonal to IPO11
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- Human cerebral cortex tissue; RT-4 and U-251 MG cell lysates; U-2 OS cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab221615 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 113 kDa.|
|IHC-P||1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionFunctions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of UBE2E3, and of RPL12.
Sequence similaritiesBelongs to the importin beta family.
Contains 15 HEAT repeats.
Contains 1 importin N-terminal domain.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- Imp11 antibody
- Importin 11 antibody
- Importin-11 antibody
Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling IPO11 with ab221615 at 1/2500 dilution.
All lanes : Anti-IPO11 antibody (ab221615) at 1/500 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Predicted band size: 113 kDa
Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized U-2 OS cells labeling IPO11 with ab221615 at 4 μg/ml (green).
ab221615 has not yet been referenced specifically in any publications.