Anti-IPO5 antibody (ab88695)
Key features and details
- Mouse polyclonal to IPO5
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-IPO5 antibody
See all IPO5 primary antibodies -
Description
Mouse polyclonal to IPO5 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant full length protein within Human IPO5. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (AAH45640.1)
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Positive control
- WB: Rat brain tissue lysate; HeLa cell lysate; Karyopherin (importin) beta 3 transfected 293T cell lysate ICC/IF: HeLa cell
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 124 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 124 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. -
Sequence similarities
Belongs to the importin beta family.
Contains 6 HEAT repeats.
Contains 1 importin N-terminal domain. -
Cellular localization
Cytoplasm. Nucleus. Nucleus > nucleolus. Nucleus; nuclear rim. Found particularly in the nuclear rim and nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 3843 Human
- Entrez Gene: 306182 Rat
- Omim: 602008 Human
- SwissProt: O00410 Human
- Unigene: 712598 Human
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Alternative names
- DKFZp686O1576 antibody
- FLJ43041 antibody
- IMB3 antibody
see all
Images
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Anti-IPO5 antibody (ab88695) at 1 µg/ml + rat brain tissue lysate at 50 µg
Predicted band size: 124 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted? -
Anti-IPO5 antibody (ab88695) at 1 µg/ml + HeLa cell lysate at 50 µg
Predicted band size: 124 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted? -
All lanes : Anti-IPO5 antibody (ab88695) at 1 µg/ml
Lane 1 : Karyopherin (importin) beta 3 transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 124 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted? -
ab88695, at 10 µg/ml, staining Karyopherin (importin) beta 3 in HeLa cell by immunofluorescence.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab88695 has not yet been referenced specifically in any publications.