Anti-IPP antibody (ab236772)
Key features and details
- Rabbit polyclonal to IPP
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-IPP antibody
See all IPP primary antibodies -
Description
Rabbit polyclonal to IPP -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
Recombinant fragment corresponding to Human IPP aa 400-550.
Database link: Q9Y573 -
Positive control
- WB: Rat kidney tissue lysate. IHC-P: Human kidney tissue. ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236772 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/50 - 1/200.
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IHC-P |
1/20 - 1/200.
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WB |
1/500 - 1/5000.
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Notes |
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ICC/IF
1/50 - 1/200. |
IHC-P
1/20 - 1/200. |
WB
1/500 - 1/5000. |
Target
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Relevance
Intracisternal A particle promoted polypeptide (IPP) is a member of the kelch family of proteins, characterized by a 50 amino acid repeat which interacts with actin. The kelch domain of IPP consists of six C terminal tandem arranged repeats. IPP may play a role in organizing the actin cytoskeleton. IPP also contains an N terminal POZ protein protein interaction domain. The POZ domain (also called BTB domain) is present near the N terminus of a fraction of zinc finger proteins and in proteins that contain the pfam01344 motif such as kelch and pox virus proteins. The BTB/POZ domain mediates homomeric dimerization and in some instances heteromeric dimerization. POZ domains from several zinc finger proteins have been shown to mediate transcriptional repression and to interact with components of histone deacetylase co repressor complexes including N-coR and SMRT. -
Cellular localization
Cytoplasm, cytoskeleton. -
Database links
- Entrez Gene: 3652 Human
- Entrez Gene: 16351 Mouse
- Entrez Gene: 298439 Rat
- Omim: 147485 Human
- SwissProt: Q9Y573 Human
- SwissProt: P28575 Mouse
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Alternative names
- Actin binding protein IPP antibody
- Intracisternal A particle promoted polypeptide antibody
- IPP POZ antibody
see all
Images
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Anti-IPP antibody (ab236772) at 1/500 dilution + Rat kidney tissue lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution -
Paraffin-embedded human kidney tissue stained for IPP with ab236772 at a 1/100 dilution in immunohistochemical analysis.
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HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for IPP using ab236772 at a dilution of 1/100 in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab236772 has not yet been referenced specifically in any publications.