- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-IPP antibody [EPR15575]
See all IPP primary antibodies -
DescriptionRabbit monoclonal [EPR15575] to IPP
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, WBmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Recombinant fragment within Human IPP aa 200-350. The exact sequence is proprietary.
Database link: Q9Y573 -
Positive control
- HepG2, Jurkat and Human testis lysates: mouse brain heart and kidney lysates: Rat heart and kidney lysates: Human placenta and Mouse kidney tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR15575
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
- HepG2 whole cell lysate (ab166833)
- Mouse kidney normal tissue lysate - total protein (ab29305)
- Jurkat whole cell lysate (ab30128)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse heart normal tissue lysate - total protein (ab30291)
- Jurkat whole cell lysate (ab7899)
- HepG2 whole cell lysate (ab7900)
Applications
Our Abpromise guarantee covers the use of ab192266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| WB | 1/1000 - 1/2000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa). |
Target
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RelevanceIntracisternal A particle promoted polypeptide (IPP) is a member of the kelch family of proteins, characterized by a 50 amino acid repeat which interacts with actin. The kelch domain of IPP consists of six C terminal tandem arranged repeats. IPP may play a role in organizing the actin cytoskeleton. IPP also contains an N terminal POZ protein protein interaction domain. The POZ domain (also called BTB domain) is present near the N terminus of a fraction of zinc finger proteins and in proteins that contain the pfam01344 motif such as kelch and pox virus proteins. The BTB/POZ domain mediates homomeric dimerization and in some instances heteromeric dimerization. POZ domains from several zinc finger proteins have been shown to mediate transcriptional repression and to interact with components of histone deacetylase co repressor complexes including N-coR and SMRT.
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Cellular localizationCytoplasm, cytoskeleton.
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Database links
- Entrez Gene: 3652 Human
- Entrez Gene: 16351 Mouse
- Entrez Gene: 298439 Rat
- Omim: 147485 Human
- SwissProt: Q9Y573 Human
- SwissProt: P28575 Mouse
- Unigene: 699548 Human
- Unigene: 229516 Rat
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Alternative names
- Actin binding protein IPP antibody
- Intracisternal A particle promoted polypeptide antibody
- IPP POZ antibody
see all
Images
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![Western blot - Anti-IPP antibody [EPR15575] (ab192266)](https://www.abcam.com/ps/products/192/ab192266/Images/ab192266-230252-192266.JPG)
Western blot - Anti-IPP antibody [EPR15575] (ab192266)All lanes : Anti-IPP antibody [EPR15575] (ab192266) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Human testis lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDa -
![Western blot - Anti-IPP antibody [EPR15575] (ab192266)](https://www.abcam.com/ps/products/192/ab192266/Images/ab192266-230251-1922662.JPG)
Western blot - Anti-IPP antibody [EPR15575] (ab192266)All lanes : Anti-IPP antibody [EPR15575] (ab192266) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Rat heart lysate
Lane 5 : Rat kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDaBlocking/dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IPP antibody [EPR15575] (ab192266)](https://www.abcam.com/ps/products/192/ab192266/Images/ab192266-230250-1922663.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IPP antibody [EPR15575] (ab192266)Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling IPP with ab192266 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Negative control also shown.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IPP antibody [EPR15575] (ab192266)](https://www.abcam.com/ps/products/192/ab192266/Images/ab192266-230249-1922664.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IPP antibody [EPR15575] (ab192266)Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling IPP with ab192266 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Negative control also shown.
References
ab192266 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
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