Recombinant
RabMAb

Recombinant Anti-IPP antibody [EPR15575] (ab192266)

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Overview

  • Product name

    Anti-IPP antibody [EPR15575]
    See all IPP primary antibodies

  • Description

    Rabbit monoclonal [EPR15575] to IPP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human IPP aa 200-350. The exact sequence is proprietary.
    Database link: Q9Y573

  • Positive control

    • HepG2, Jurkat and Human testis lysates: mouse brain heart and kidney lysates: Rat heart and kidney lysates: Human placenta and Mouse kidney tissue.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15575

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab192266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000 - 1/2000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).

Target

  • Relevance

    Intracisternal A particle promoted polypeptide (IPP) is a member of the kelch family of proteins, characterized by a 50 amino acid repeat which interacts with actin. The kelch domain of IPP consists of six C terminal tandem arranged repeats. IPP may play a role in organizing the actin cytoskeleton. IPP also contains an N terminal POZ protein protein interaction domain. The POZ domain (also called BTB domain) is present near the N terminus of a fraction of zinc finger proteins and in proteins that contain the pfam01344 motif such as kelch and pox virus proteins. The BTB/POZ domain mediates homomeric dimerization and in some instances heteromeric dimerization. POZ domains from several zinc finger proteins have been shown to mediate transcriptional repression and to interact with components of histone deacetylase co repressor complexes including N-coR and SMRT.

  • Cellular localization

    Cytoplasm, cytoskeleton.

  • Database links

    • Alternative names

      • Actin binding protein IPP antibody
      • Intracisternal A particle promoted polypeptide antibody
      • IPP POZ antibody
      • Kelch like protein 27 antibody
      • KLHL27 antibody
      • MIPP protein antibody
      see all

    Images

    • Western blot - Anti-IPP antibody [EPR15575] (ab192266)
      Western blot - Anti-IPP antibody [EPR15575] (ab192266)
      All lanes : Anti-IPP antibody [EPR15575] (ab192266) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : Human testis lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 65 kDa
      Observed band size: 65 kDa

    • Western blot - Anti-IPP antibody [EPR15575] (ab192266)
      Western blot - Anti-IPP antibody [EPR15575] (ab192266)
      All lanes : Anti-IPP antibody [EPR15575] (ab192266) at 1/1000 dilution

      Lane 1 : Mouse brain lysate
      Lane 2 : Mouse heart lysate
      Lane 3 : Mouse kidney lysate
      Lane 4 : Rat heart lysate
      Lane 5 : Rat kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 65 kDa
      Observed band size: 65 kDa



      Blocking/dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IPP antibody [EPR15575] (ab192266)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IPP antibody [EPR15575] (ab192266)

      Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling IPP with ab192266 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Negative control also shown.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IPP antibody [EPR15575] (ab192266)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IPP antibody [EPR15575] (ab192266)

      Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling IPP with ab192266 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Negative control also shown.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    References

    ab192266 has not yet been referenced specifically in any publications.

    Publishing research using ab192266? Please let us know so that we can cite the reference in this datasheet.

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