RecombinantRabMAb

Recombinant Anti-IPP antibody [EPR15575] (ab192266)

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Western blot - Anti-IPP antibody [EPR15575] (ab192266)
  • Western blot - Anti-IPP antibody [EPR15575] (ab192266)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IPP antibody [EPR15575] (ab192266)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IPP antibody [EPR15575] (ab192266)
  • Anti-IPP antibody [EPR15575] (ab192266)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15575] to IPP
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-IPP antibody [EPR15575]
    See all IPP primary antibodies

  • Description

    Rabbit monoclonal [EPR15575] to IPP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human IPP aa 200-350. The exact sequence is proprietary.
    Database link: Q9Y573

  • Positive control

    • HepG2, Jurkat and Human testis lysates: mouse brain heart and kidney lysates: Rat heart and kidney lysates: Human placenta and Mouse kidney tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15575

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab192266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000 - 1/2000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).

Target

  • Relevance

    Intracisternal A particle promoted polypeptide (IPP) is a member of the kelch family of proteins, characterized by a 50 amino acid repeat which interacts with actin. The kelch domain of IPP consists of six C terminal tandem arranged repeats. IPP may play a role in organizing the actin cytoskeleton. IPP also contains an N terminal POZ protein protein interaction domain. The POZ domain (also called BTB domain) is present near the N terminus of a fraction of zinc finger proteins and in proteins that contain the pfam01344 motif such as kelch and pox virus proteins. The BTB/POZ domain mediates homomeric dimerization and in some instances heteromeric dimerization. POZ domains from several zinc finger proteins have been shown to mediate transcriptional repression and to interact with components of histone deacetylase co repressor complexes including N-coR and SMRT.

  • Cellular localization

    Cytoplasm, cytoskeleton.

  • Database links

    • Alternative names

      • Actin binding protein IPP antibody
      • Intracisternal A particle promoted polypeptide antibody
      • IPP POZ antibody
      • Kelch like protein 27 antibody
      • KLHL27 antibody
      • MIPP protein antibody
      see all

    Images

    • Western blot - Anti-IPP antibody [EPR15575] (ab192266)
      Western blot - Anti-IPP antibody [EPR15575] (ab192266)
      All lanes : Anti-IPP antibody [EPR15575] (ab192266) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : Human testis lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 65 kDa
      Observed band size: 65 kDa

    • Western blot - Anti-IPP antibody [EPR15575] (ab192266)
      Western blot - Anti-IPP antibody [EPR15575] (ab192266)
      All lanes : Anti-IPP antibody [EPR15575] (ab192266) at 1/1000 dilution

      Lane 1 : Mouse brain lysate
      Lane 2 : Mouse heart lysate
      Lane 3 : Mouse kidney lysate
      Lane 4 : Rat heart lysate
      Lane 5 : Rat kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 65 kDa
      Observed band size: 65 kDa



      Blocking/dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IPP antibody [EPR15575] (ab192266)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IPP antibody [EPR15575] (ab192266)

      Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling IPP with ab192266 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Negative control also shown.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IPP antibody [EPR15575] (ab192266)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IPP antibody [EPR15575] (ab192266)

      Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling IPP with ab192266 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Negative control also shown.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-IPP antibody [EPR15575] (ab192266)
      Anti-IPP antibody [EPR15575] (ab192266)

    References (0)

    Publishing research using ab192266? Please let us know so that we can cite the reference in this datasheet.

    ab192266 has not yet been referenced specifically in any publications.

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