Recombinant
RabMAb

Recombinant Anti-IPP antibody [EPR15575] - BSA and Azide free (ab251133)

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Overview

  • Product name

    Anti-IPP antibody [EPR15575] - BSA and Azide free
    See all IPP primary antibodies

  • Description

    Rabbit monoclonal [EPR15575] to IPP - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human IPP aa 200-350. The exact sequence is proprietary.
    Database link: Q9Y573

  • General notes

    Ab251133 is the carrier-free version of ab192266. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab251133 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab251133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).

Target

  • Relevance

    Intracisternal A particle promoted polypeptide (IPP) is a member of the kelch family of proteins, characterized by a 50 amino acid repeat which interacts with actin. The kelch domain of IPP consists of six C terminal tandem arranged repeats. IPP may play a role in organizing the actin cytoskeleton. IPP also contains an N terminal POZ protein protein interaction domain. The POZ domain (also called BTB domain) is present near the N terminus of a fraction of zinc finger proteins and in proteins that contain the pfam01344 motif such as kelch and pox virus proteins. The BTB/POZ domain mediates homomeric dimerization and in some instances heteromeric dimerization. POZ domains from several zinc finger proteins have been shown to mediate transcriptional repression and to interact with components of histone deacetylase co repressor complexes including N-coR and SMRT.

  • Cellular localization

    Cytoplasm, cytoskeleton.

  • Database links

    • Alternative names

      • Actin binding protein IPP antibody
      • Intracisternal A particle promoted polypeptide antibody
      • IPP POZ antibody
      • Kelch like protein 27 antibody
      • KLHL27 antibody
      • MIPP protein antibody
      see all

    References

    ab251133 has not yet been referenced specifically in any publications.

    Publishing research using ab251133? Please let us know so that we can cite the reference in this datasheet.

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