RecombinantRabMAb

Recombinant Anti-IPP antibody [EPR15575] - BSA and Azide free (ab251133)

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Western blot - Anti-IPP antibody [EPR15575] - BSA and Azide free (ab251133)
  • Western blot - Anti-IPP antibody [EPR15575] - BSA and Azide free (ab251133)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IPP antibody [EPR15575] - BSA and Azide free (ab251133)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IPP antibody [EPR15575] - BSA and Azide free (ab251133)
  • Anti-IPP antibody [EPR15575] - BSA and Azide free (ab251133)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15575] to IPP - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-IPP antibody [EPR15575] - BSA and Azide free
    See all IPP primary antibodies

  • Description

    Rabbit monoclonal [EPR15575] to IPP - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251133 is the carrier-free version of ab192266. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab251133 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab251133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).

Target

  • Relevance

    Intracisternal A particle promoted polypeptide (IPP) is a member of the kelch family of proteins, characterized by a 50 amino acid repeat which interacts with actin. The kelch domain of IPP consists of six C terminal tandem arranged repeats. IPP may play a role in organizing the actin cytoskeleton. IPP also contains an N terminal POZ protein protein interaction domain. The POZ domain (also called BTB domain) is present near the N terminus of a fraction of zinc finger proteins and in proteins that contain the pfam01344 motif such as kelch and pox virus proteins. The BTB/POZ domain mediates homomeric dimerization and in some instances heteromeric dimerization. POZ domains from several zinc finger proteins have been shown to mediate transcriptional repression and to interact with components of histone deacetylase co repressor complexes including N-coR and SMRT.

  • Cellular localization

    Cytoplasm, cytoskeleton.

  • Database links

    • Alternative names

      • Actin binding protein IPP antibody
      • Intracisternal A particle promoted polypeptide antibody
      • IPP POZ antibody
      • Kelch like protein 27 antibody
      • KLHL27 antibody
      • MIPP protein antibody
      see all

    Images

    • Western blot - Anti-IPP antibody [EPR15575] - BSA and Azide free (ab251133)
      Western blot - Anti-IPP antibody [EPR15575] - BSA and Azide free (ab251133)
      All lanes : Anti-IPP antibody [EPR15575] (ab192266) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : Human testis tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 65 kDa
      Observed band size: 65 kDa



      This data was developed using ab192266, the same antibody clone in a different buffer formulation.

    • Western blot - Anti-IPP antibody [EPR15575] - BSA and Azide free (ab251133)
      Western blot - Anti-IPP antibody [EPR15575] - BSA and Azide free (ab251133)
      All lanes : Anti-IPP antibody [EPR15575] (ab192266) at 1/1000 dilution

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Mouse heart tissue lysate
      Lane 3 : Mouse kidney tissue lysate
      Lane 4 : Rat heart tissue lysate
      Lane 5 : Rat kidney tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 65 kDa
      Observed band size: 65 kDa



      This data was developed using ab192266, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IPP antibody [EPR15575] - BSA and Azide free (ab251133)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IPP antibody [EPR15575] - BSA and Azide free (ab251133)
      This data was developed using ab192266, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling IPP with ab192266 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Negative control also shown. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IPP antibody [EPR15575] - BSA and Azide free (ab251133)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IPP antibody [EPR15575] - BSA and Azide free (ab251133)
      This data was developed using ab192266, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling IPP with ab192266 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Negative control also shown. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    • Anti-IPP antibody [EPR15575] - BSA and Azide free (ab251133)
      Anti-IPP antibody [EPR15575] - BSA and Azide free (ab251133)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab251133? Please let us know so that we can cite the reference in this datasheet.

    ab251133 has not yet been referenced specifically in any publications.

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