Anti-IPPK/IPK1 antibody (ab196781)
Key features and details
- Rabbit polyclonal to IPPK/IPK1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-IPPK/IPK1 antibody
See all IPPK/IPK1 primary antibodies -
Description
Rabbit polyclonal to IPPK/IPK1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human IPPK/IPK1 (internal sequence).
Database link: Q9H8X2 -
Positive control
- RAW, K562, LOVO and 3T3 cell extracts. Human brain tissue. HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
PBS without Mg2+ and Ca2+. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196781 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 56 kDa.
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ICC/IF |
1/100 - 1/500.
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IHC-P |
1/50 - 1/100.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 56 kDa. |
ICC/IF
1/100 - 1/500. |
IHC-P
1/50 - 1/100. |
Target
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Function
Phosphorylates Ins(1,3,4,5,6)P5 at position 2 to form Ins(1,2,3,4,5,6)P6 (InsP6 or phytate). InsP6 is involved in many processes such as mRNA export, non-homologous end-joining, endocytosis, ion channel regulation. It also protects cells from TNF-alpha-induced apoptosis. -
Tissue specificity
Ubiquitously expressed, with high expression in heart, brain, testis and placenta. -
Sequence similarities
Belongs to the IPK1 type 2 family. -
Domain
The EXKPK motif is conserved in inositol-pentakisphosphate 2-kinases of both family 1 and 2. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 64768 Human
- Entrez Gene: 75678 Mouse
- SwissProt: Q9H8X2 Human
- SwissProt: Q6P1C1 Mouse
- Unigene: 459896 Human
- Unigene: 713775 Human
- Unigene: 390586 Mouse
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Alternative names
- 3 antibody
- 4 antibody
- 5 antibody
see all
Images
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All lanes : Anti-IPPK/IPK1 antibody (ab196781) at 1/500 dilution
Lane 1 : RAW cell extracts
Lane 2 : K562 cell extracts
Lane 3 : LOVO cell extracts
Lane 4 : 3T3 cell extract
Lane 5 : Cell extracts with synthesized peptide
Predicted band size: 56 kDa -
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling IPPK/IPK1 with ab196781 at 1/50 dilution. The image on the right is pre-incubated with synthesized peptide.
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Immunofluorescent analysis of HepG2 cells labeling IPPK/IPK1 with ab196781 at 1/100 dilution. The image on the right is pre-incubated with synthesized peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab196781 has not yet been referenced specifically in any publications.