Anti-IPS1 antibody (ab240581)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-IPS1 antibody
See all IPS1 primary antibodies -
Description
Rabbit polyclonal to IPS1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Orangutan
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Immunogen
Synthetic peptide within Human IPS1 aa 1383-1433. The exact sequence is proprietary. (GeneID 9677).
Database link: Q6PFW1 -
Positive control
- WB: HeLa, HEK-293T and Jurkat cell lysate. IP: HEK-293T cell lysate.
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General notes
This product was previously labelled as HISPPD2A
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab240581 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Relevance
Bifunctional inositol kinase that catalyzes the formation of diphosphoinositol pentakisphosphate (InsP7 or PP-InsP5) and bi-diphosphoinositol tetrakisphosphate (InsP8 or PP2-InsP4). Converts inositolitol hexakisphosphate (InsP6) to InsP7. Also able to convert InsP7 to InsP8. Probably specifically mediates the formation of 4PP-InsP5 and 6PP-InsP5 InsP7 isomers but not of 5PP-IP5 InsP7 isomer. Activated when cells are exposed to hyperosmotic stress. -
Cellular localization
Cytoplasm, cytosol and Plasma membrane -
Database links
- Entrez Gene: 510684 Cow
- Entrez Gene: 9677 Human
- Omim: 610979 Human
- SwissProt: A7Z050 Cow
- SwissProt: Q6PFW1 Human
- SwissProt: Q5RDF1 Orangutan
- Unigene: 156814 Human
- Unigene: 679911 Human
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Alternative names
- diphosphoinositol pentakisphosphate kinase 1 antibody
- HISPPD2A antibody
- histidine acid phosphatase domain containing 2A antibody
see all
Images
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Western blot - Anti-IPS1 antibody (ab240581)All lanes : Anti-IPS1 antibody (ab240581) at 1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Exposure time: 30 secondsDetection: Chemiluminescence with an exposure time of 30 seconds.
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Immunoprecipitation - Anti-IPS1 antibody (ab240581)IPS1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer, using ab240581 at 6 μg per reaction. For western blotting, ab240581 was used at 0.4 µg/ml.
Lane 1: ab240581 used for IP at 6 µg per reaction.
Lane 2: Control IgG.Detection: Chemiluminescence with an exposure time of 30 seconds.
Datasheets and documents
References
ab240581 has not yet been referenced specifically in any publications.
