Anti-IPS1 antibody (ab240581)
Key features and details
- Rabbit polyclonal to IPS1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IPS1 antibody
See all IPS1 primary antibodies -
Description
Rabbit polyclonal to IPS1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Orangutan
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Immunogen
Synthetic peptide within Human IPS1 aa 1383-1433. The exact sequence is proprietary. (GeneID 9677).
Database link: Q6PFW1 -
Positive control
- WB: HeLa, HEK-293T and Jurkat cell lysate. IP: HEK-293T cell lysate.
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General notes
This product was previously labelled as HISPPD2A
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab240581 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Relevance
Bifunctional inositol kinase that catalyzes the formation of diphosphoinositol pentakisphosphate (InsP7 or PP-InsP5) and bi-diphosphoinositol tetrakisphosphate (InsP8 or PP2-InsP4). Converts inositolitol hexakisphosphate (InsP6) to InsP7. Also able to convert InsP7 to InsP8. Probably specifically mediates the formation of 4PP-InsP5 and 6PP-InsP5 InsP7 isomers but not of 5PP-IP5 InsP7 isomer. Activated when cells are exposed to hyperosmotic stress. -
Cellular localization
Cytoplasm, cytosol and Plasma membrane -
Database links
- Entrez Gene: 510684 Cow
- Entrez Gene: 9677 Human
- Omim: 610979 Human
- SwissProt: A7Z050 Cow
- SwissProt: Q6PFW1 Human
- SwissProt: Q5RDF1 Orangutan
- Unigene: 156814 Human
- Unigene: 679911 Human
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Alternative names
- diphosphoinositol pentakisphosphate kinase 1 antibody
- HISPPD2A antibody
- histidine acid phosphatase domain containing 2A antibody
see all
Images
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Western blot - Anti-IPS1 antibody (ab240581)All lanes : Anti-IPS1 antibody (ab240581) at 1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Exposure time: 30 secondsDetection: Chemiluminescence with an exposure time of 30 seconds.
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Immunoprecipitation - Anti-IPS1 antibody (ab240581)IPS1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer, using ab240581 at 6 μg per reaction. For western blotting, ab240581 was used at 0.4 µg/ml.
Lane 1: ab240581 used for IP at 6 µg per reaction.
Lane 2: Control IgG.Detection: Chemiluminescence with an exposure time of 30 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab240581 has not yet been referenced specifically in any publications.
