Overview

  • Product name
    Anti-IPT antibody [EPR12517]
    See all IPT primary antibodies
  • Description
    Rabbit monoclonal [EPR12517] to IPT
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human IPT aa 100-200 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9H3H1

  • Positive control
    • WB: Jurkat and Raji cell lysates and human fetal lung tissue lysate. Flow Cyt: Raji cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab174308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 53 kDa.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function
      Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 of both cytosolic and mitochondrial tRNAs, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A).
    • Sequence similarities
      Belongs to the IPP transferase family.
      Contains 1 matrin-type zinc finger.
    • Cellular localization
      Cytoplasm and Mitochondrion.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2310075G14Rik antibody
      • AI314189 antibody
      • AI314635 antibody
      • AV099619 antibody
      • FLJ20061 antibody
      • hGRO1 antibody
      • IPP transferase antibody
      • IPPT antibody
      • IPT antibody
      • IPTase antibody
      • Isopentenyl diphosphate:tRNA isopentenyltransferase antibody
      • Isopentenyl-diphosphate:tRNA isopentenyltransferase antibody
      • MGC149242 antibody
      • MGC149243 antibody
      • mitochondrial antibody
      • MOD5 antibody
      • MOD5_HUMAN antibody
      • OTTMUSP00000009314 antibody
      • Putative protein TR2IT1 antibody
      • RP23-121J14.6 antibody
      • TRIT 1 antibody
      • TRIT1 antibody
      • tRNA dimethylallyltransferase antibody
      • tRNA isopentenylpyrophosphate transferase antibody
      • tRNA isopentenyltransferase 1 antibody
      • tRNA isopentenyltransferase antibody
      • tRNA isopentenyltransferase mitochondrial antibody
      see all

    Images

    • All lanes : Anti-IPT antibody [EPR12517] (ab174308) at 1/1000 dilution

      Lane 1 : Jurakt cell lysate
      Lane 2 : Fetal lung lysate
      Lane 3 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 53 kDa

    • Flow cytometric analysis of permeabilized Raji cells labeling IPT with ab174308 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).

    References

    ab174308 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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