Overview

  • Product name
    IPTG, Allosteric lac repressor protein inhibitor
  • Description
    Allosteric lac repressor protein inhibitor
  • Biological description
    Allosteric lac repressor protein inhibitor. Induces β-galactosidase (LacZ) expression. Used in combination with X-gal for blue/white selection of recombinant bacteria.
  • Purity
    > 99%
  • CAS Number
    367-93-1
  • Chemical structure
    Chemical Structure

Properties

  • Chemical name
    (3R,4R,5S)-2-(Hydroxymethyl)-6-propan-2-ylsulfanyloxane-3,4,5-triol
  • Molecular weight
    238.09
  • Molecular formula
    C9H18O5S
  • PubChem identifier
    656894
  • Storage instructions
    Store at +4°C. The product can be stored for up to 12 months.
  • Solubility overview
    Soluble in water to 100 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES
    CC(C)SC1C(C(C(C(O1)CO)O)O)O
  • Source

    Synthetic

  • Research areas

References

This product has been referenced in:
  • Marbach A & Bettenbrock K lac operon induction in Escherichia coli: Systematic comparison of IPTG and TMG induction and influence of the transacetylase LacA. J Biotechnol 157:82-8 (2012). Read more (PubMed: 22079752) »
  • Sambrook J & Russell DW Screening Bacterial Colonies Using X-gal and IPTG: a-Complementation. CSH Protoc 2006: (2006). Read more (PubMed: 22485386) »
  • Hansen LH  et al. The effect of the lacY gene on the induction of IPTG inducible promoters, studied in Escherichia coli and Pseudomonas fluorescens. Curr Microbiol 36:341-7 (1998). Read more (PubMed: 9608745) »

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