Anti-IQ motif containing B1 antibody (ab69927)
Key features and details
- Mouse polyclonal to IQ motif containing B1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-IQ motif containing B1 antibody
See all IQ motif containing B1 primary antibodies -
Description
Mouse polyclonal to IQ motif containing B1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human IQ motif containing B1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
-
Positive control
- IQ motif containing B1 transfected 293T cell lysate
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
-
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab69927 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 54 kDa).
Predicted molecular weight for isoform 2: 54 kDa. |
Notes |
---|
WB
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 54 kDa). Predicted molecular weight for isoform 2: 54 kDa. |
Target
-
Relevance
Interacts with calmodulin. -
Cellular localization
Photoreceptor connecting cilium -
Database links
- Entrez Gene: 9657 Human
- Omim: 609237 Human
- SwissProt: Q15051 Human
-
Alternative names
- IQ calmodulin binding motif containing 1 antibody
- IQ calmodulin binding motif containing protein 1 antibody
- IQCB1 antibody
see all
Images
-
All lanes : Anti-IQ motif containing B1 antibody (ab69927) at 1/500 dilution
Lane 1 : IQ motif containing B1 transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 54 kDa
Observed band size: 69 kDa why is the actual band size different from the predicted?
Datasheets and documents
-
Datasheet download
References (2)
ab69927 has been referenced in 2 publications.
- Hossain D et al. Requirement of NPHP5 in the hierarchical assembly of basal feet associated with basal bodies of primary cilia. Cell Mol Life Sci 77:195-212 (2020). PubMed: 31177295
- Zhang Y et al. BBS mutations modify phenotypic expression of CEP290-related ciliopathies. Hum Mol Genet N/A:N/A (2013). PubMed: 23943788