Recombinant
RabMAb

Recombinant Anti-IQGAP2 antibody [EPR13382] - BSA and Azide free (ab240242)

Overview

  • Product name

    Anti-IQGAP2 antibody [EPR13382] - BSA and Azide free
    See all IQGAP2 primary antibodies
  • Description

    Rabbit monoclonal [EPR13382] to IQGAP2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human IQGAP2 aa 1300-1500. The exact sequence is proprietary.
    Database link: Q13576

  • General notes

    Ab240242 is the carrier-free version of ab181127. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240242 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 181 kDa (predicted molecular weight: 181 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Binds to activated CDC42 and RAC1 but does not seem to stimulate their GTPase activity. Associates with calmodulin.
  • Tissue specificity

    Isoform 2 expression is enhanced in testis.
  • Sequence similarities

    Contains 1 CH (calponin-homology) domain.
    Contains 3 IQ domains.
    Contains 1 Ras-GAP domain.
    Contains 1 WW domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • IQ motif containing GTPase activating protein 2 antibody
    • IQGA2_HUMAN antibody
    • Iqgap2 antibody
    • Ras GTPase activating-like protein antibody
    • Ras GTPase-activating-like protein IQGAP2 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue labeling IQGAP2 with ab181127 at a 1/100 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181127).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin embedded Human liver tissue labeling IQGAP2 ab181127 at a 1/100 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181127).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin embedded Human colonic carcinoma tissue labeling IQGAP2 with ab181127 at a 1/100 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181127).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

References

ab240242 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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