Key features and details
- Rabbit polyclonal to IRAK-1 (phospho T209)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-IRAK-1 (phospho T209) antibody
See all IRAK-1 primary antibodies
DescriptionRabbit polyclonal to IRAK-1 (phospho T209)
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
- WB: 293T cell lysate. IHC-P: Mouse placenta tissue.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab218130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100 - 1/1000. Predicted molecular weight: 77 kDa.|
FunctionBinds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1.
Tissue specificityIsoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.
Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 protein kinase domain.
modificationsAutophosphorylated or is transphosphorylated by IRAK4 following recruitment to the IL-1RI. In the case of isoform 1, this is linked to ubiquitination and degradation.
Polyubiquitinated; after cell stimulation with IL-1-beta. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'.
- Information by UniProt
- AA48924 antibody
- Il1rak antibody
- Interleukin 1 receptor associated kinase 1 antibody
Immunohistochemical analysis of formalin-fixed paraffin-embedded mouse placenta tissue, labeling IRAK-1 using ab218130 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Anti-IRAK-1 (phospho T209) antibody (ab218130) at 1/300 dilution + 293T cell lysates
Goat anti-rabbit IgG antibody (H+L) HRP-conjugated at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 77 kDa
ab218130 has been referenced in 1 publication.
- Gomez D et al. Interleukin-1ß has atheroprotective effects in advanced atherosclerotic lesions of mice. Nat Med 24:1418-1429 (2018). PubMed: 30038218