Overview

  • Product name

    Anti-IRAK-1 (phospho T209) antibody
    See all IRAK-1 primary antibodies
  • Description

    Rabbit polyclonal to IRAK-1 (phospho T209)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Mouse IRAK-1 aa 195-225 (phospho T209) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    PSPFCWPFCEISQGTCNFSEELRIGEGGFGC


    Database link: Q62406

  • Positive control

    • WB: 293T cell lysate. IHC-P: Mouse placenta tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab218130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/1000. Predicted molecular weight: 77 kDa.

Target

  • Function

    Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1.
  • Tissue specificity

    Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated or is transphosphorylated by IRAK4 following recruitment to the IL-1RI. In the case of isoform 1, this is linked to ubiquitination and degradation.
    Polyubiquitinated; after cell stimulation with IL-1-beta. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'.
  • Information by UniProt
  • Database links

  • Alternative names

    • AA48924 antibody
    • Il1rak antibody
    • Interleukin 1 receptor associated kinase 1 antibody
    • Interleukin-1 receptor-associated kinase 1 antibody
    • IRAK antibody
    • IRAK-1 antibody
    • Irak1 antibody
    • IRAK1-S antibody
    • IRAK1_HUMAN antibody
    • mPLK antibody
    • OTTHUMP00000026014 antibody
    • OTTHUMP00000026015 antibody
    • OTTHUMP00000026020 antibody
    • OTTHUMP00000180621 antibody
    • Pelle antibody
    • Pelle homolog antibody
    • Pelle-like protein kinase antibody
    • Plpk antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded mouse placenta tissue, labeling IRAK-1 using ab218130 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

  • Anti-IRAK-1 (phospho T209) antibody (ab218130) at 1/300 dilution + 293T cell lysates

    Secondary
    Goat anti-rabbit IgG antibody (H+L) HRP-conjugated at 1/5000 dilution

    Developed using the ECL technique.

    Predicted band size: 77 kDa

References

This product has been referenced in:

  • Gomez D  et al. Interleukin-1ß has atheroprotective effects in advanced atherosclerotic lesions of mice. Nat Med 24:1418-1429 (2018). Read more (PubMed: 30038218) »
See 1 Publication for this product

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