Anti-IRAK2 antibody (ab6148)
Key features and details
- Rabbit polyclonal to IRAK2
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-IRAK2 antibody
See all IRAK2 primary antibodies -
Description
Rabbit polyclonal to IRAK2 -
Host species
Rabbit -
Specificity
Anti-IRAK2 has no cross response to IRAK. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide, corresponding to amino acids 546-564 of Human IRAK2.
-
General notes
The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2 (1). Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kB activation. IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kB activation (2,3).Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. IRAK2 is expressed in a variety of human tissues.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified IgG was prepared by affinity chromatography. -
Primary antibody notes
The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2 (1). Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kB activation. IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kB activation (2,3).Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. IRAK2 is expressed in a variety of human tissues. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 2 µg/ml.
|
|
| WB |
1/500 - 1/1000. Predicted molecular weight: 65 kDa.
|
| Notes |
|---|
|
ICC/IF
Use a concentration of 2 µg/ml. |
|
WB
1/500 - 1/1000. Predicted molecular weight: 65 kDa. |
Target
-
Function
Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. -
Tissue specificity
Expressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 death domain.
Contains 1 protein kinase domain. -
Domain
The protein kinase domain is predicted to be catalytically inactive. - Information by UniProt
-
Database links
- Entrez Gene: 3656 Human
- Omim: 603304 Human
- SwissProt: O43187 Human
- Unigene: 449207 Human
-
Alternative names
- il1rak2 antibody
- Interleukin 1 receptor associated kinase 2 antibody
- Interleukin 1 receptor associated kinase like 2 antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (ab6148)Immunofluorescent analysis of Hela cells staining IRAK2 with ab6148 at 20µg/ml
-
Western blot - Anti-IRAK2 antibody (ab6148)All lanes : Anti-IRAK2 antibody (ab6148) at 2 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : K562 whole cell lysate
Predicted band size: 65 kDa
Observed band size: 65 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (ab6148)ab6148 at 2µg/ml staining IRAK2 in Hela cells by ICC/IF
-
Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (ab6148)Immunofluorescence of IRAK-2 in Hela cells using ab6148 at 10 ug/ml.
Protocols
Datasheets and documents
-
Datasheet download
References (0)
ab6148 has not yet been referenced specifically in any publications.
