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    irak2-antibody-ab6148.pdf

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Immunology Innate Immunity Cytokines Interleukins
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Anti-IRAK2 antibody (ab6148)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)

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Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (ab6148)
  • Western blot - Anti-IRAK2 antibody (ab6148)
  • Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (ab6148)
  • Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (ab6148)

Key features and details

  • Rabbit polyclonal to IRAK2
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-IRAK2 antibody
    See all IRAK2 primary antibodies
  • Description

    Rabbit polyclonal to IRAK2
  • Host species

    Rabbit
  • Specificity

    Anti-IRAK2 has no cross response to IRAK.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide, corresponding to amino acids 546-564 of Human IRAK2.

  • General notes


    The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2 (1). Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kB activation. IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kB activation (2,3).Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. IRAK2 is expressed in a variety of human tissues.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Purified IgG was prepared by affinity chromatography.
  • Primary antibody notes

    The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2 (1). Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kB activation. IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kB activation (2,3).Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. IRAK2 is expressed in a variety of human tissues.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Immunology
    • Innate Immunity
    • TLR Signaling

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • K-562 whole cell lysate (ab7911)
  • Recombinant Protein

    • Recombinant human IRAK2 protein (ab60859)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
Application Abreviews Notes
ICC/IF
Use a concentration of 2 µg/ml.
WB
1/500 - 1/1000. Predicted molecular weight: 65 kDa.
Notes
ICC/IF
Use a concentration of 2 µg/ml.
WB
1/500 - 1/1000. Predicted molecular weight: 65 kDa.

Target

  • Function

    Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization.
  • Tissue specificity

    Expressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes.
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
    Contains 1 death domain.
    Contains 1 protein kinase domain.
  • Domain

    The protein kinase domain is predicted to be catalytically inactive.
  • Target information above from: UniProt accession O43187 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3656 Human
    • Omim: 603304 Human
    • SwissProt: O43187 Human
    • Unigene: 449207 Human
    • Alternative names

      • il1rak2 antibody
      • Interleukin 1 receptor associated kinase 2 antibody
      • Interleukin 1 receptor associated kinase like 2 antibody
      • Interleukin-1 receptor-associated kinase-like 2 antibody
      • IRAK 2 antibody
      • IRAK-2 antibody
      • Irak2 antibody
      • IRAK2_HUMAN antibody
      • MGC150550 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (ab6148)
      Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (ab6148)
      Immunofluorescent analysis of Hela cells staining IRAK2 with ab6148 at 20µg/ml
    • Western blot - Anti-IRAK2 antibody (ab6148)
      Western blot - Anti-IRAK2 antibody (ab6148)
      All lanes : Anti-IRAK2 antibody (ab6148) at 2 µg/ml

      Lane 1 : HeLa whole cell lysate
      Lane 2 : K562 whole cell lysate

      Predicted band size: 65 kDa
      Observed band size: 65 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (ab6148)
      Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (ab6148)

      ab6148 at 2µg/ml staining IRAK2 in Hela cells by ICC/IF

    • Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (ab6148)
      Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (ab6148)
      Immunofluorescence of IRAK-2 in Hela cells using ab6148 at 10 ug/ml.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab6148? Please let us know so that we can cite the reference in this datasheet.

    ab6148 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Immunoprecipitation abreview for Anti-IRAK2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Immuno-precipitation step
    Protein G
    Sample
    Human Cell lysate - whole cell (HEK293)
    Specification
    HEK293
    Total protein in input
    1000 µg
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 07 2013

    Question

    I was wondering had you assessed the anti IRAK-2 antibody for its ability to immunoprecipitate IRAK-2 ??

    Read More

    Abcam community

    Verified customer

    Asked on May 11 2001

    Answer

    We do not have any reports of this antibody being used for immunoprecipitation

    Read More

    Abcam Scientific Support

    Answered on May 14 2001

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