Anti-IRAK2 antibody (ab6148)
Key features and details
- Rabbit polyclonal to IRAK2
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IRAK2 antibody
See all IRAK2 primary antibodies -
Description
Rabbit polyclonal to IRAK2 -
Host species
Rabbit -
Specificity
Anti-IRAK2 has no cross response to IRAK. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide, corresponding to amino acids 546-564 of Human IRAK2.
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General notes
The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2 (1). Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kB activation. IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kB activation (2,3).Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. IRAK2 is expressed in a variety of human tissues.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified IgG was prepared by affinity chromatography. -
Primary antibody notes
The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2 (1). Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kB activation. IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kB activation (2,3).Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. IRAK2 is expressed in a variety of human tissues. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
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| WB |
Human
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| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 2 µg/ml.
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| WB |
1/500 - 1/1000. Predicted molecular weight: 65 kDa.
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| Notes |
|---|
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ICC/IF
Use a concentration of 2 µg/ml. |
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WB
1/500 - 1/1000. Predicted molecular weight: 65 kDa. |
Target
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Function
Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. -
Tissue specificity
Expressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 death domain.
Contains 1 protein kinase domain. -
Domain
The protein kinase domain is predicted to be catalytically inactive. - Information by UniProt
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Database links
- Entrez Gene: 3656 Human
- Omim: 603304 Human
- SwissProt: O43187 Human
- Unigene: 449207 Human
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Alternative names
- il1rak2 antibody
- Interleukin 1 receptor associated kinase 2 antibody
- Interleukin 1 receptor associated kinase like 2 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (ab6148)Immunofluorescent analysis of Hela cells staining IRAK2 with ab6148 at 20µg/ml
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Western blot - Anti-IRAK2 antibody (ab6148)All lanes : Anti-IRAK2 antibody (ab6148) at 2 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : K562 whole cell lysate
Predicted band size: 65 kDa
Observed band size: 65 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (ab6148)ab6148 at 2µg/ml staining IRAK2 in Hela cells by ICC/IF
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Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (ab6148)Immunofluorescence of IRAK-2 in Hela cells using ab6148 at 10 ug/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab6148 has not yet been referenced specifically in any publications.