- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-IRAK2 antibody
See all IRAK2 primary antibodies -
DescriptionRabbit polyclonal to IRAK2
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Host speciesRabbit
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SpecificityAnti-IRAK2 has no cross response to IRAK.
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Tested applicationsSuitable for: ICC/IF, WB, IPmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide, corresponding to amino acids 546-564 of Human IRAK2.
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Positive control
- Whole cell lysate from HeLa or K562 cells.
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General notes
The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2 (1). Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kB activation. IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kB activation (2,3).Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. IRAK2 is expressed in a variety of human tissues.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPBS with 0.02% sodium azide
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Concentration information loading... -
PurityAffinity purified
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Purification notesPurified IgG was prepared by affinity chromatography.
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Primary antibody notesThe pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2 (1). Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kB activation. IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kB activation (2,3).Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. IRAK2 is expressed in a variety of human tissues.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab6148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 2 µg/ml. | |
| WB | 1/500 - 1/1000. Predicted molecular weight: 65 kDa.Can be blocked with IRAK2 peptide (ab6232). | |
| IP | Use at 1 µg/mg of lysate. |
Target
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FunctionBinds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization.
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Tissue specificityExpressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes.
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Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 death domain.
Contains 1 protein kinase domain. -
DomainThe protein kinase domain is predicted to be catalytically inactive.
- Information by UniProt
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Database links
- Entrez Gene: 3656 Human
- Omim: 603304 Human
- SwissProt: O43187 Human
- Unigene: 449207 Human
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Alternative names
- il1rak2 antibody
- Interleukin 1 receptor associated kinase 2 antibody
- Interleukin 1 receptor associated kinase like 2 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (ab6148)Immunofluorescent analysis of Hela cells staining IRAK2 with ab6148 at 20µg/ml -
Western blot - Anti-IRAK2 antibody (ab6148)All lanes : Anti-IRAK2 antibody (ab6148) at 2 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : K562 whole cell lysate
Predicted band size: 65 kDa
Observed band size: 65 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (ab6148)ab6148 at 2µg/ml staining IRAK2 in Hela cells by ICC/IF
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Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (ab6148)Immunofluorescence of IRAK-2 in Hela cells using ab6148 at 10 ug/ml.
Protocols
References
ab6148 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"