Product nameAnti-IRAK2 antibody
See all IRAK2 primary antibodies
DescriptionRabbit polyclonal to IRAK2
SpecificityThis antibody reacts with IRAK2, it does not react with IRAK
Tested applicationsSuitable for: ICC/IF, ICC, IP, WBmore details
Species reactivityReacts with: Mouse, Human
A synthetic peptide corresponding to 17 amino acids near the C terminus of mouse IRAK2a, (which is common to all four isoforms).
- RAW2647 whole cell lysate and A20 cells
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab62419 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution (PMID 19224918).
WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionBinds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization.
Tissue specificityExpressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes.
Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 death domain.
Contains 1 protein kinase domain.
DomainThe protein kinase domain is predicted to be catalytically inactive.
- Information by UniProt
- il1rak2 antibody
- Interleukin 1 receptor associated kinase 2 antibody
- Interleukin 1 receptor associated kinase like 2 antibody
Anti-IRAK antibody (ab62419) reactivity with reduced wildtype (WT) or IRAK2 knockout (KO) mouse embryonic fibroblast (MEF) cell lysate. After SDS-PAGE, membranes were blocked in 5% milk for 1h at 25°C before incubation with ab62419 (1µg/ml dilution in 5% milk in TBS/0.5% Tween) for 16h at 4ºC. The blot was then incubated with an anti-Rabbit HRP-conjugated secondary antibody before developing with ECL.
Immunofluorescent analysis of IRAK2 expression in human spleen cells with ab62419 at 20µg/ml.
Lane 1 : Anti-IRAK2 antibody (ab62419) at 0.5 µg/ml
Lane 2 : Anti-IRAK2 antibody (ab62419) at 1 µg/ml
Lane 3 : Anti-IRAK2 antibody (ab62419) at 2 µg/ml
All lanes : RAW264.7 whole cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 65 kDa
Observed band size: 65 kDa
Immunocytochemistry of IRAK2 in A-20 cells with ab62419 antibody at 1 µg/ml.
Immunofluorescence of IRAK2 in A20 cells using ab62419 at 10 ug/ml.
This product has been referenced in:
- Zhou H et al. IRAK2 directs stimulus-dependent nuclear export of inflammatory mRNAs. Elife 6:N/A (2017). Read more (PubMed: 28990926) »
- Pan L et al. Shear stress induces human aortic endothelial cell apoptosis via interleukin-1 receptor-associated kinase 2-induced endoplasmic reticulum stress. Mol Med Rep 16:7205-7212 (2017). Read more (PubMed: 28944871) »