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    irak4-antibody-2h9-ab119942.pdf

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Immunology Innate Immunity Cytokines Interleukins
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Anti-IRAK4 antibody [2H9] (ab119942)

  • Datasheet
Submit a review Submit a question References (3)

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Western blot - Anti-IRAK4 antibody [2H9] (ab119942)
  • Western blot - Anti-IRAK4 antibody [2H9] (ab119942)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAK4 antibody [2H9] (ab119942)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAK4 antibody [2H9] (ab119942)
  • Flow Cytometry - Anti-IRAK4 antibody [2H9] (ab119942)
  • ELISA - Anti-IRAK4 antibody [2H9] (ab119942)
  • Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody [2H9] (ab119942)

Key features and details

  • Mouse monoclonal [2H9] to IRAK4
  • Suitable for: WB, ELISA, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Human, African green monkey, Recombinant fragment
  • Isotype: IgG1

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Overview

  • Product name

    Anti-IRAK4 antibody [2H9]
    See all IRAK4 primary antibodies
  • Description

    Mouse monoclonal [2H9] to IRAK4
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data
  • Immunogen

    Recombinant fragment, corresponding to amino acids 21-198 of Human IRAK4

  • Positive control

    • Recombinant IRAK4 protein; THP-1, Hela, K562, MCF7, RAW264.7, Jurkat and Cos7 cell lysates; Human lung cancer amd kidney cancer tissues; Hela cells This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: HepG2.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    2H9
  • Isotype

    IgG1
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Immunology
    • Innate Immunity
    • TLR Signaling

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant human IRAK4 protein (ab79739)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab119942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Human
WB
Human
Recombinant fragment
Application Abreviews Notes
WB
1/500 - 1/2000. Predicted molecular weight: 52 kDa.
ELISA
1/10000.
IHC-P
1/200 - 1/1000.
Flow Cyt
1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF
1/100.
Notes
WB
1/500 - 1/2000. Predicted molecular weight: 52 kDa.
ELISA
1/10000.
IHC-P
1/200 - 1/1000.
Flow Cyt
1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF
1/100.

Target

  • Function

    Required for the efficient recruitment of IRAK1 to the IL-1 receptor complex following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Phosphorylates IRAK1.
  • Involvement in disease

    Defects in IRAK4 are the cause of recurrent isolated invasive pneumococcal disease type 1 (IPD1) [MIM:610799]. Recurrent invasive pneumococcal disease (IPD) is defined as two episodes of IPD occurring at least 1 month apart, whether caused by the same or different serotypes or strains. Recurrent IPD occurs in at least 2% of patients in most series, making IPD the most important known risk factor for subsequent IPD.
    Defects in IRAK4 are the cause of IRAK4 deficiency (IRAK4D) [MIM:607676]. IRAK4 deficiency causes extracellular pyogenic bacterial and fungal infections in otherwise healthy children.
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
    Contains 1 death domain.
    Contains 1 protein kinase domain.
  • Target information above from: UniProt accession Q9NWZ3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 51135 Human
    • Entrez Gene: 266632 Mouse
    • Omim: 606883 Human
    • SwissProt: Q9NWZ3 Human
    • SwissProt: Q8R4K2 Mouse
    • Unigene: 138499 Human
    • Unigene: 422858 Mouse
    • Alternative names

      • IL-1 receptor-associated kinase 4 antibody
      • Interleukin 1 receptor associated kinase 4 mutant form 1 antibody
      • Interleukin-1 receptor-associated kinase 4 antibody
      • Interleukin1 receptor associated kinase 4 antibody
      • IPD1 antibody
      • IRAK 4 antibody
      • IRAK-4 antibody
      • IRAK4 antibody
      • IRAK4 mutated form 1 antibody
      • IRAK4_HUMAN antibody
      • LOC 51135 antibody
      • NY REN 64 antibody
      • NY REN 64 antigen antibody
      • NY-REN-64 antibody
      • REN64 antibody
      • Renal carcinoma antigen NY-REN-64 antibody
      see all

    Images

    • Western blot - Anti-IRAK4 antibody [2H9] (ab119942)
      Western blot - Anti-IRAK4 antibody [2H9] (ab119942)
      All lanes : Anti-IRAK4 antibody [2H9] (ab119942) at 1/500 dilution

      Lane 1 : THP-1 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : MCF7 cell lysate
      Lane 5 : RAW264.7 cell lysate
      Lane 6 : Jurkat cell lysate
      Lane 7 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate

      Predicted band size: 52 kDa

    • Western blot - Anti-IRAK4 antibody [2H9] (ab119942)
      Western blot - Anti-IRAK4 antibody [2H9] (ab119942)
      Anti-IRAK4 antibody [2H9] (ab119942) at 1/500 dilution + Recombinant IRAK4 protein

      Predicted band size: 52 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAK4 antibody [2H9] (ab119942)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAK4 antibody [2H9] (ab119942)
      ab119942, at 1/200 dilution, staining IRAK4 in paraffin-embedded Human lung cancer tissue by Immunohistochemistry with DAB staining.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAK4 antibody [2H9] (ab119942)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAK4 antibody [2H9] (ab119942)
      ab119942, at 1/200 dilution, staining IRAK4 in paraffin-embedded Human kidney cancer tissue by Immunohistochemistry with DAB staining.
    • Flow Cytometry - Anti-IRAK4 antibody [2H9] (ab119942)
      Flow Cytometry - Anti-IRAK4 antibody [2H9] (ab119942)
      Flow cytometric analysis of Hela cells using ab119942 at 1/200 dilution (blue) and negative control (red).
    • ELISA - Anti-IRAK4 antibody [2H9] (ab119942)
      ELISA - Anti-IRAK4 antibody [2H9] (ab119942)
      ELISA using ab119942.
    • Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody [2H9] (ab119942)
      Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody [2H9] (ab119942)

      ab119942 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab119942 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (3)

    Publishing research using ab119942? Please let us know so that we can cite the reference in this datasheet.

    ab119942 has been referenced in 3 publications.

    • Tan Y & Kagan JC Biochemical Isolation of the Myddosome from Murine Macrophages. Methods Mol Biol 1714:79-95 (2018). PubMed: 29177857
    • Zhang L  et al. The role of Toll-like receptor 3 and 4 in regulating the function of mesenchymal stem cells isolated from umbilical cord. Int J Mol Med 35:1003-10 (2015). PubMed: 25695694
    • Murayama A  et al. A subclone of HuH-7 with enhanced intracellular hepatitis C virus production and evasion of virus related-cell cycle arrest. PLoS One 7:e52697 (2012). PubMed: 23285155

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