Anti-IRAK4 antibody [2H9] (ab119942)

Mouse monoclonal IRAK4 antibody [2H9]. Validated in WB, ELISA, IHC, Flow Cyt, ICC/IF and tested in Mouse, Human, Monkey. Cited in 1 publication(s). Immunogen corresponding to recombinant fragment.

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Overview

  • Product name

    Anti-IRAK4 antibody [2H9]
    See all IRAK4 primary antibodies

  • Description

    Mouse monoclonal [2H9] to IRAK4

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ELISA, IHC-P, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human, Monkey

  • Immunogen

    Recombinant fragment, corresponding to amino acids 21-198 of Human IRAK4

  • Positive control

    • Recombinant IRAK4 protein; THP-1, Hela, K562, MCF7, RAW264.7, Jurkat and Cos7 cell lysates; Human lung cancer amd kidney cancer tissues; Hela cells This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: HepG2.

Properties

Applications

Our Abpromise guarantee covers the use of ab119942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 52 kDa.
ELISA 1/10000.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

Target

  • Function

    Required for the efficient recruitment of IRAK1 to the IL-1 receptor complex following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Phosphorylates IRAK1.

  • Involvement in disease

    Defects in IRAK4 are the cause of recurrent isolated invasive pneumococcal disease type 1 (IPD1) [MIM:610799]. Recurrent invasive pneumococcal disease (IPD) is defined as two episodes of IPD occurring at least 1 month apart, whether caused by the same or different serotypes or strains. Recurrent IPD occurs in at least 2% of patients in most series, making IPD the most important known risk factor for subsequent IPD.
    Defects in IRAK4 are the cause of IRAK4 deficiency (IRAK4D) [MIM:607676]. IRAK4 deficiency causes extracellular pyogenic bacterial and fungal infections in otherwise healthy children.

  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
    Contains 1 death domain.
    Contains 1 protein kinase domain.
  • Information by UniProt

  • Database links

    • Alternative names

      • IL-1 receptor-associated kinase 4 antibody
      • Interleukin 1 receptor associated kinase 4 mutant form 1 antibody
      • Interleukin-1 receptor-associated kinase 4 antibody
      • Interleukin1 receptor associated kinase 4 antibody
      • IPD1 antibody
      • IRAK 4 antibody
      • IRAK-4 antibody
      • IRAK4 antibody
      • IRAK4 mutated form 1 antibody
      • IRAK4_HUMAN antibody
      • LOC 51135 antibody
      • NY REN 64 antibody
      • NY REN 64 antigen antibody
      • NY-REN-64 antibody
      • REN64 antibody
      • Renal carcinoma antigen NY-REN-64 antibody
      see all

    Images

    • Western blot - Anti-IRAK4 antibody [2H9] (ab119942)
      Western blot - Anti-IRAK4 antibody [2H9] (ab119942)
      All lanes : Anti-IRAK4 antibody [2H9] (ab119942) at 1/500 dilution

      Lane 1 : THP-1 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : MCF7 cell lysate
      Lane 5 : RAW264.7 cell lysate
      Lane 6 : Jurkat cell lysate
      Lane 7 : COS7 cell lysate

      Predicted band size: 52 kDa

    • Western blot - Anti-IRAK4 antibody [2H9] (ab119942)
      Western blot - Anti-IRAK4 antibody [2H9] (ab119942)
      Anti-IRAK4 antibody [2H9] (ab119942) at 1/500 dilution + Recombinant IRAK4 protein

      Predicted band size: 52 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAK4 antibody [2H9] (ab119942)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAK4 antibody [2H9] (ab119942)
      ab119942, at 1/200 dilution, staining IRAK4 in paraffin-embedded Human lung cancer tissue by Immunohistochemistry with DAB staining.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAK4 antibody [2H9] (ab119942)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAK4 antibody [2H9] (ab119942)
      ab119942, at 1/200 dilution, staining IRAK4 in paraffin-embedded Human kidney cancer tissue by Immunohistochemistry with DAB staining.
    • Flow Cytometry - Anti-IRAK4 antibody [2H9] (ab119942)
      Flow Cytometry - Anti-IRAK4 antibody [2H9] (ab119942)
      Flow cytometric analysis of Hela cells using ab119942 at 1/200 dilution (blue) and negative control (red).
    • ELISA - Anti-IRAK4 antibody [2H9] (ab119942)
      ELISA - Anti-IRAK4 antibody [2H9] (ab119942)
      ELISA using ab119942.
    • Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody [2H9] (ab119942)
      Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody [2H9] (ab119942)

      ab119942 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab119942 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References

    This product has been referenced in:

    • Tan Y & Kagan JC Biochemical Isolation of the Myddosome from Murine Macrophages. Methods Mol Biol 1714:79-95 (2018). Read more (PubMed: 29177857) »
    • Zhang L  et al. The role of Toll-like receptor 3 and 4 in regulating the function of mesenchymal stem cells isolated from umbilical cord. Int J Mol Med 35:1003-10 (2015). Read more (PubMed: 25695694) »
    See all 2 Publications for this product

    Publishing research using ab119942? Please let us know so that we can cite the reference in this datasheet.

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