Anti-IRAK4 antibody [2H9] (ab119942)
![Western blot - Anti-IRAK4 antibody [2H9] (ab119942)](https://www.abcam.com/ps/products/119/ab119942/Images/ab119942-133510-anti-irak4-antibody-2h9-western-blot.jpg "Western blot - Anti-IRAK4 antibody [2H9] (ab119942)")
Key features and details
- Mouse monoclonal [2H9] to IRAK4
- Suitable for: WB, ELISA, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human, African green monkey, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-IRAK4 antibody [2H9]
See all IRAK4 primary antibodies -
Description
Mouse monoclonal [2H9] to IRAK4 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB HumanRecombinant fragment
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Immunogen
Recombinant fragment, corresponding to amino acids 21-198 of Human IRAK4
-
Positive control
- Recombinant IRAK4 protein; THP-1, Hela, K562, MCF7, RAW264.7, Jurkat and Cos7 cell lysates; Human lung cancer amd kidney cancer tissues; Hela cells This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: HepG2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
2H9 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab119942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| WB |
Human
Recombinant fragment
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/2000. Predicted molecular weight: 52 kDa.
|
|
| ELISA |
1/10000.
|
|
| IHC-P |
1/200 - 1/1000.
|
|
| Flow Cyt |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| ICC/IF |
1/100.
|
| Notes |
|---|
|
WB
1/500 - 1/2000. Predicted molecular weight: 52 kDa. |
|
ELISA
1/10000. |
|
IHC-P
1/200 - 1/1000. |
|
Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
ICC/IF
1/100. |
Target
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Function
Required for the efficient recruitment of IRAK1 to the IL-1 receptor complex following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Phosphorylates IRAK1. -
Involvement in disease
Defects in IRAK4 are the cause of recurrent isolated invasive pneumococcal disease type 1 (IPD1) [MIM:610799]. Recurrent invasive pneumococcal disease (IPD) is defined as two episodes of IPD occurring at least 1 month apart, whether caused by the same or different serotypes or strains. Recurrent IPD occurs in at least 2% of patients in most series, making IPD the most important known risk factor for subsequent IPD.
Defects in IRAK4 are the cause of IRAK4 deficiency (IRAK4D) [MIM:607676]. IRAK4 deficiency causes extracellular pyogenic bacterial and fungal infections in otherwise healthy children. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 death domain.
Contains 1 protein kinase domain. - Information by UniProt
-
Database links
- Entrez Gene: 51135 Human
- Entrez Gene: 266632 Mouse
- Omim: 606883 Human
- SwissProt: Q9NWZ3 Human
- SwissProt: Q8R4K2 Mouse
- Unigene: 138499 Human
- Unigene: 422858 Mouse
-
Alternative names
- IL-1 receptor-associated kinase 4 antibody
- Interleukin 1 receptor associated kinase 4 mutant form 1 antibody
- Interleukin-1 receptor-associated kinase 4 antibody
see all
Images
-
Western blot - Anti-IRAK4 antibody [2H9] (ab119942)All lanes : Anti-IRAK4 antibody [2H9] (ab119942) at 1/500 dilution
Lane 1 : THP-1 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lane 4 : MCF7 cell lysate
Lane 5 : RAW264.7 cell lysate
Lane 6 : Jurkat cell lysate
Lane 7 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate
Predicted band size: 52 kDa -
![Western blot - Anti-IRAK4 antibody [2H9] (ab119942)](https://www.abcam.com/ps/products/119/ab119942/Images/ab119942-133511-anti-irak4-antibody-2h9-western-blot.jpg)
Western blot - Anti-IRAK4 antibody [2H9] (ab119942)Anti-IRAK4 antibody [2H9] (ab119942) at 1/500 dilution + Recombinant IRAK4 protein
Predicted band size: 52 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAK4 antibody [2H9] (ab119942)](https://www.abcam.com/ps/products/119/ab119942/Images/ab119942-133509-anti-irak4-antibody-2h9-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAK4 antibody [2H9] (ab119942)ab119942, at 1/200 dilution, staining IRAK4 in paraffin-embedded Human lung cancer tissue by Immunohistochemistry with DAB staining. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAK4 antibody [2H9] (ab119942)](https://www.abcam.com/ps/products/119/ab119942/Images/ab119942-133508-anti-irak4-antibody-2h9-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAK4 antibody [2H9] (ab119942)ab119942, at 1/200 dilution, staining IRAK4 in paraffin-embedded Human kidney cancer tissue by Immunohistochemistry with DAB staining. -
![Flow Cytometry - Anti-IRAK4 antibody [2H9] (ab119942)](https://www.abcam.com/ps/products/119/ab119942/Images/ab119942-133507-anti-irak4-antibody-2h9-flow-cytometry.jpg)
Flow Cytometry - Anti-IRAK4 antibody [2H9] (ab119942)Flow cytometric analysis of Hela cells using ab119942 at 1/200 dilution (blue) and negative control (red). -
![ELISA - Anti-IRAK4 antibody [2H9] (ab119942)](https://www.abcam.com/ps/products/119/ab119942/Images/ab119942-133506-anti-irak4-antibody-2h9-elisa.jpg)
ELISA - Anti-IRAK4 antibody [2H9] (ab119942)ELISA using ab119942. -
![Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody [2H9] (ab119942)](https://www.abcam.com/ps/products/119/ab119942/Images/ab119942-133505-anti-irak4-antibody-2h9-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody [2H9] (ab119942)ab119942 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab119942 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (3)
ab119942 has been referenced in 3 publications.
- Tan Y & Kagan JC Biochemical Isolation of the Myddosome from Murine Macrophages. Methods Mol Biol 1714:79-95 (2018). PubMed: 29177857
- Zhang L et al. The role of Toll-like receptor 3 and 4 in regulating the function of mesenchymal stem cells isolated from umbilical cord. Int J Mol Med 35:1003-10 (2015). PubMed: 25695694
- Murayama A et al. A subclone of HuH-7 with enhanced intracellular hepatitis C virus production and evasion of virus related-cell cycle arrest. PLoS One 7:e52697 (2012). PubMed: 23285155
