Overview

  • Product name

  • Description

    Rabbit polyclonal to IRAK4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Recombinant fragment within Human IRAK4 (internal sequence). The exact sequence is proprietary.
    Database link: Q69FE3

  • Positive control

    • WB: U-87 MG whole cell lysate. ICC/IF: HeLa cells. IHC-P: HeLa xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab228754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 52 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Required for the efficient recruitment of IRAK1 to the IL-1 receptor complex following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Phosphorylates IRAK1.
  • Involvement in disease

    Defects in IRAK4 are the cause of recurrent isolated invasive pneumococcal disease type 1 (IPD1) [MIM:610799]. Recurrent invasive pneumococcal disease (IPD) is defined as two episodes of IPD occurring at least 1 month apart, whether caused by the same or different serotypes or strains. Recurrent IPD occurs in at least 2% of patients in most series, making IPD the most important known risk factor for subsequent IPD.
    Defects in IRAK4 are the cause of IRAK4 deficiency (IRAK4D) [MIM:607676]. IRAK4 deficiency causes extracellular pyogenic bacterial and fungal infections in otherwise healthy children.
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
    Contains 1 death domain.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • IL-1 receptor-associated kinase 4 antibody
    • Interleukin 1 receptor associated kinase 4 mutant form 1 antibody
    • Interleukin-1 receptor-associated kinase 4 antibody
    • Interleukin1 receptor associated kinase 4 antibody
    • IPD1 antibody
    • IRAK 4 antibody
    • IRAK-4 antibody
    • IRAK4 antibody
    • IRAK4 mutated form 1 antibody
    • IRAK4_HUMAN antibody
    • LOC 51135 antibody
    • NY REN 64 antibody
    • NY REN 64 antigen antibody
    • NY-REN-64 antibody
    • REN64 antibody
    • Renal carcinoma antigen NY-REN-64 antibody
    see all

Images

  • Anti-IRAK4 antibody (ab228754) at 1/1000 dilution + U87-MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 30 µg

    Predicted band size: 52 kDa



    10% SDS-PAGE gel.

  • 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for IRAK4 (green) using ab228754 at 1/100 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

  • Paraffin-embedded HeLa (human epithelial cell line from cervix adenocarcinoma) xenograft tissue stained for IRAK4 using ab228754 at 1/500 dilution in immunohistochemical analysis.

References

ab228754 has not yet been referenced specifically in any publications.

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