- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-IRAK4 antibody
See all IRAK4 primary antibodies -
DescriptionRabbit polyclonal to IRAK4
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Host speciesRabbit
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SpecificityThis antibody is specific to IRAK4 and does not cross react with other IRAK proteins.
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Tested applicationsSuitable for: ICC, ICC/IF, WBmore details
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Species reactivityReacts with: Sheep, Human
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Immunogen
Synthetic peptide corresponding to amino acids at the carboxy terminus end of IRAK-4 (Human).
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Positive control
- HeLa whole cell lysate.
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General notesStable for one year.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
Applications
Our Abpromise guarantee covers the use of ab5985 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | ||
| ICC/IF | ||
| WB |
Application notes
ICC/IF: Use at a concentration of 10 µg/ml.
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 50 kDa. Can be blocked with IRAK4 peptide (448-460) (ab39782).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 50 kDa. Can be blocked with IRAK4 peptide (448-460) (ab39782).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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FunctionRequired for the efficient recruitment of IRAK1 to the IL-1 receptor complex following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Phosphorylates IRAK1.
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Involvement in diseaseDefects in IRAK4 are the cause of recurrent isolated invasive pneumococcal disease type 1 (IPD1) [MIM:610799]. Recurrent invasive pneumococcal disease (IPD) is defined as two episodes of IPD occurring at least 1 month apart, whether caused by the same or different serotypes or strains. Recurrent IPD occurs in at least 2% of patients in most series, making IPD the most important known risk factor for subsequent IPD.
Defects in IRAK4 are the cause of IRAK4 deficiency (IRAK4D) [MIM:607676]. IRAK4 deficiency causes extracellular pyogenic bacterial and fungal infections in otherwise healthy children. -
Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 death domain.
Contains 1 protein kinase domain. - Information by UniProt
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Database links
- Entrez Gene: 51135 Human
- Omim: 606883 Human
- SwissProt: Q9NWZ3 Human
- Unigene: 138499 Human
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Alternative names
- IL-1 receptor-associated kinase 4 antibody
- Interleukin 1 receptor associated kinase 4 mutant form 1 antibody
- Interleukin-1 receptor-associated kinase 4 antibody
see all
Images
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Western blot - Anti-IRAK4 antibody (ab5985)Western blot analysis of IRAK4 in HeLa cell lysate with ab5985 at 1 (lane 1), 2 (lane 2), and 4 (lane 3)µ g/ml, respectively. Western blot analysis of IRAK4 in HeLa cell lysate with ab5985 at 1 (lane 1), 2 (lane 2), and 4 (lane 3) µg/ml, respectively. -
Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (ab5985)ab5985 at 10µg/ml staining IRAK-4 in K562 by ICC/IF
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Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (ab5985)This image was kindly supplied by Dr Mahesh Shivananjappa by Abreviewab5985 at a 1/100 dilution staining IRAK4 in human PMN cells by Immunocytochemistry/ Immunofluorescence incubated for 4 hours at 37°C. Fixed with PFA. Blocked using 2% BSA for 1 hour at 22°C. Secondary used at 1/250 polyclonal Goat anti-rabbit conjugated to Alexa Fluor 568.
Left image: DAPI staining nuclei (blue)Middle image: IRAK4 (red)
Right image: Overlay -
Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (ab5985)Immunofluorescence of IRAK-4 in K562 cells using ab5985 at 10 ug/ml.
References
This product has been referenced in:
- Kallapur SG et al. Pulmonary and systemic endotoxin tolerance in preterm fetal sheep exposed to chorioamnionitis. J Immunol 179:8491-9 (2007). WB ; Sheep . Read more (PubMed: 18056396) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"