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    irak4-antibody-ab5985.pdf

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Immunology Innate Immunity Cytokines Interleukins
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Anti-IRAK4 antibody (ab5985)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (1)

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Western blot - Anti-IRAK4 antibody (ab5985)
  • Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (ab5985)
  • Western blot - Anti-IRAK4 antibody (ab5985)
  • Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (ab5985)
  • Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (ab5985)

Key features and details

  • Rabbit polyclonal to IRAK4
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-IRAK4 antibody
    See all IRAK4 primary antibodies
  • Description

    Rabbit polyclonal to IRAK4
  • Host species

    Rabbit
  • Specificity

    This antibody is specific to IRAK4 and does not cross react with other IRAK proteins.
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human IRAK4 (C terminal).
    (Peptide available as ab39782)

  • Positive control

    • WB: Daudi, HepG2 and HeLa whole cell lysate. ICC/IF: K562 cells
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. The product can be stored for up to 12 months.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Immunology
    • Innate Immunity
    • TLR Signaling

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human IRAK4 protein (ab79739)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab5985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use a concentration of 10 µg/ml.
WB (1)
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 50 kDa.
Notes
ICC/IF
Use a concentration of 10 µg/ml.
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 50 kDa.

Target

  • Function

    Required for the efficient recruitment of IRAK1 to the IL-1 receptor complex following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Phosphorylates IRAK1.
  • Involvement in disease

    Defects in IRAK4 are the cause of recurrent isolated invasive pneumococcal disease type 1 (IPD1) [MIM:610799]. Recurrent invasive pneumococcal disease (IPD) is defined as two episodes of IPD occurring at least 1 month apart, whether caused by the same or different serotypes or strains. Recurrent IPD occurs in at least 2% of patients in most series, making IPD the most important known risk factor for subsequent IPD.
    Defects in IRAK4 are the cause of IRAK4 deficiency (IRAK4D) [MIM:607676]. IRAK4 deficiency causes extracellular pyogenic bacterial and fungal infections in otherwise healthy children.
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
    Contains 1 death domain.
    Contains 1 protein kinase domain.
  • Target information above from: UniProt accession Q9NWZ3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 51135 Human
    • Omim: 606883 Human
    • SwissProt: Q9NWZ3 Human
    • Unigene: 138499 Human
    • Alternative names

      • IL-1 receptor-associated kinase 4 antibody
      • Interleukin 1 receptor associated kinase 4 mutant form 1 antibody
      • Interleukin-1 receptor-associated kinase 4 antibody
      • Interleukin1 receptor associated kinase 4 antibody
      • IPD1 antibody
      • IRAK 4 antibody
      • IRAK-4 antibody
      • IRAK4 antibody
      • IRAK4 mutated form 1 antibody
      • IRAK4_HUMAN antibody
      • LOC 51135 antibody
      • NY REN 64 antibody
      • NY REN 64 antigen antibody
      • NY-REN-64 antibody
      • REN64 antibody
      • Renal carcinoma antigen NY-REN-64 antibody
      see all

    Images

    • Western blot - Anti-IRAK4 antibody (ab5985)
      Western blot - Anti-IRAK4 antibody (ab5985)
      All lanes : Anti-IRAK4 antibody (ab5985) at 1 µg/ml

      Lane 1 : Daudi cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
    • Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (ab5985)
      Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (ab5985)

      Immunofluorescent analysis of 4% paraformaldehydefixed K562 Cells labeling IRAK4 with ab9585 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

    • Western blot - Anti-IRAK4 antibody (ab5985)
      Western blot - Anti-IRAK4 antibody (ab5985)
      Lane 1 : Anti-IRAK4 antibody (ab5985) at 1 µg/ml
      Lane 2 : Anti-IRAK4 antibody (ab5985) at 2 µg/ml
      Lane 3 : Anti-IRAK4 antibody (ab5985) at 4 µg/ml

      All lanes : HeLa cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution


      Primary incbation: 1 hour at room temperature in 5% NFDM/TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (ab5985)
      Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (ab5985)

      Immunocytochemical analysis of K562 cells labeling IRAK4 with ab5985 at 10 μg/mL. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

    • Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (ab5985)
      Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (ab5985)This image was kindly supplied by Dr Mahesh Shivananjappa by Abreview
      ab5985 at a 1/100 dilution staining IRAK4 in human PMN cells by Immunocytochemistry/ Immunofluorescence incubated for 4 hours at 37°C. Fixed with PFA. Blocked using 2% BSA for 1 hour at 22°C. Secondary used at 1/250 polyclonal Goat anti-rabbit conjugated to Alexa Fluor 568.
      Left image: DAPI staining nuclei (blue)
      Middle image: IRAK4 (red)
      Right image: Overlay

      See Abreview

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab5985? Please let us know so that we can cite the reference in this datasheet.

    ab5985 has been referenced in 1 publication.

    • Kallapur SG  et al. Pulmonary and systemic endotoxin tolerance in preterm fetal sheep exposed to chorioamnionitis. J Immunol 179:8491-9 (2007). WB ; Sheep . PubMed: 18056396

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry abreview for Anti-IRAK4 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cell (PMN)
    Specification
    PMN
    Fixative
    Paraformaldehyde
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 29 2009

    Western blot abreview for Anti-IRAK4 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MRC-5)
    Loading amount
    1e+006 cells
    Specification
    MRC-5
    Blocking step
    Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 17 2006

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