Anti-IRAK4 antibody (ab5985)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-IRAK4 antibody
See all IRAK4 primary antibodies -
Description
Rabbit polyclonal to IRAK4 -
Host species
Rabbit -
Specificity
This antibody is specific to IRAK4 and does not cross react with other IRAK proteins. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human IRAK4 (C terminal).
(Peptide available asab39782) -
Positive control
- WB: Daudi, HepG2 and HeLa whole cell lysate. ICC/IF: K562 cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. The product can be stored for up to 12 months. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab5985 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 10 µg/ml. | |
| WB | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 50 kDa. |
Target
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Function
Required for the efficient recruitment of IRAK1 to the IL-1 receptor complex following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Phosphorylates IRAK1. -
Involvement in disease
Defects in IRAK4 are the cause of recurrent isolated invasive pneumococcal disease type 1 (IPD1) [MIM:610799]. Recurrent invasive pneumococcal disease (IPD) is defined as two episodes of IPD occurring at least 1 month apart, whether caused by the same or different serotypes or strains. Recurrent IPD occurs in at least 2% of patients in most series, making IPD the most important known risk factor for subsequent IPD.
Defects in IRAK4 are the cause of IRAK4 deficiency (IRAK4D) [MIM:607676]. IRAK4 deficiency causes extracellular pyogenic bacterial and fungal infections in otherwise healthy children. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 death domain.
Contains 1 protein kinase domain. - Information by UniProt
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Database links
- Entrez Gene: 51135 Human
- Omim: 606883 Human
- SwissProt: Q9NWZ3 Human
- Unigene: 138499 Human
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Alternative names
- IL-1 receptor-associated kinase 4 antibody
- Interleukin 1 receptor associated kinase 4 mutant form 1 antibody
- Interleukin-1 receptor-associated kinase 4 antibody
see all
Images
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Western blot - Anti-IRAK4 antibody (ab5985)All lanes : Anti-IRAK4 antibody (ab5985) at 1 µg/ml
Lane 1 : Daudi cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution -
Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (ab5985)Immunofluorescent analysis of 4% paraformaldehydefixed K562 Cells labeling IRAK4 with ab9585 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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Western blot - Anti-IRAK4 antibody (ab5985)Lane 1 : Anti-IRAK4 antibody (ab5985) at 1 µg/ml
Lane 2 : Anti-IRAK4 antibody (ab5985) at 2 µg/ml
Lane 3 : Anti-IRAK4 antibody (ab5985) at 4 µg/ml
All lanes : HeLa cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilutionPrimary incbation: 1 hour at room temperature in 5% NFDM/TBST.
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Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (ab5985)Immunocytochemical analysis of K562 cells labeling IRAK4 with ab5985 at 10 μg/mL. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (ab5985)This image was kindly supplied by Dr Mahesh Shivananjappa by Abreviewab5985 at a 1/100 dilution staining IRAK4 in human PMN cells by Immunocytochemistry/ Immunofluorescence incubated for 4 hours at 37°C. Fixed with PFA. Blocked using 2% BSA for 1 hour at 22°C. Secondary used at 1/250 polyclonal Goat anti-rabbit conjugated to Alexa Fluor 568.
Left image: DAPI staining nuclei (blue)Middle image: IRAK4 (red)
Right image: Overlay
Datasheets and documents
References
This product has been referenced in:
- Kallapur SG et al. Pulmonary and systemic endotoxin tolerance in preterm fetal sheep exposed to chorioamnionitis. J Immunol 179:8491-9 (2007). WB ; Sheep . Read more (PubMed: 18056396) »
