Western blot abreview for Anti-IRAK4 antibody

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (MRC-5)
Loading amount
1e+006 cells
Specification
MRC-5
Blocking step
Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Other product details

Dilution
1/500
Incubation time
18 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: Alexa 680 goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680

Detection

Detection method
Li-Cor
Exposure
30 second(s)
Bands
Specific: 54 kDa
Positive control
MRC-5 cells transfected with IRAK4 plasmid constructs.

Additional data

Additional Notes
Lane 1: Invitrogen SeeBlue +2 marker
Lane 2: Invitrogen MagicMark XP marker
Lane 3: empty
Lane 4-7: MRC5 transfected with IRAK4 plasmid
Lane 8: MRC5 without transfected IRAK4plasmid
Green is anti-goat antibody
Red is anti-mouse, picking up anti-mouse GAPDH
Green marker, starting from top is 220, 120, 100, 80, 60, 50, 40, 30, 20 kb

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Submitted Oct 17 2006

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