Overview

  • Product name

    Anti-IRAKM antibody [5C3D6] - C-terminal
    See all IRAKM primary antibodies
  • Description

    Mouse monoclonal [5C3D6] to IRAKM - C-terminal
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human IRAKM aa 454-596 (C terminal). Expressed in E. Coli.
    Sequence:

    EDPPTSL KSFRCPSPLF LENVPSIPVE DDESQNNNLL PSDEGLRIDR MTQKTPFECS QSEVMFLSLD KKPESKRNEE ACNMPSSSCE ESWFPKYIVP SQDLRPYKVN IDPSSEAPGH SCRSRPVESS CSSKFSWDEY EQYKKE


    Database link: Q9Y616

  • Positive control

    • WB: Human IRAKM (aa: 454-596) recombinant protein; Human IRAKM (aa: 454-596)-hIgGFc transfected HEK293 cell lysate. FACS: A549 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab216132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/400.
WB 1/500 - 1/2000. Predicted molecular weight: 68 kDa.

Target

  • Function

    Inhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex.
  • Tissue specificity

    Expressed predominantly in peripheral blood lymphocytes.
  • Involvement in disease

    Defects in IRAK3 are associated with susceptibility to asthma-related traits type 5 (ASRT5) [MIM:611064]. Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing, dyspnea, bronchial hyperresponsiveness as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis.
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
    Contains 1 death domain.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • ASRT5 antibody
    • IL 1 receptor associated kinase M antibody
    • IL-1 receptor-associated kinase M antibody
    • IL1 receptor associated kinase M antibody
    • Interleukin 1 receptor associated kinase 3 antibody
    • Interleukin 1 receptor associated kinase M antibody
    • Interleukin-1 receptor-associated kinase 3 antibody
    • IRAK M antibody
    • IRAK-3 antibody
    • IRAK-M antibody
    • Irak3 antibody
    • IRAK3_HUMAN antibody
    see all

Images

  • Immunofluorescent analaysis of A549 cells labeling IRAKM with ab216132 at 1/100 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. 

  • All lanes : Anti-IRAKM antibody [5C3D6] - C-terminal (ab216132) at 1/500 dilution

    Lane 1 : non-transfected HEK293 cell lysate
    Lane 2 : Human IRAKM (aa: 454-596)-hIgGFc transfected HEK293 cell lysate

    Predicted band size: 68 kDa

  • Anti-IRAKM antibody [5C3D6] - C-terminal (ab216132) at 1/500 dilution + Human IRAKM (aa: 454-596) recombinant protein

    Predicted band size: 68 kDa

References

ab216132 has not yet been referenced specifically in any publications.

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