Overview

  • Product name

    Anti-IRAKM antibody - C-terminal
    See all IRAKM primary antibodies
  • Description

    Rabbit polyclonal to IRAKM - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human IRAKM (C terminal).
    Database link: Q9Y616

  • Positive control

    • Human tonsil tissue; rat liver cells; rat liver and mouse spleen lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab189128 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 68 kDa.
IHC-P Use a concentration of 2 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function

    Inhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex.
  • Tissue specificity

    Expressed predominantly in peripheral blood lymphocytes.
  • Involvement in disease

    Defects in IRAK3 are associated with susceptibility to asthma-related traits type 5 (ASRT5) [MIM:611064]. Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing, dyspnea, bronchial hyperresponsiveness as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis.
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
    Contains 1 death domain.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • ASRT5 antibody
    • IL 1 receptor associated kinase M antibody
    • IL-1 receptor-associated kinase M antibody
    • IL1 receptor associated kinase M antibody
    • Interleukin 1 receptor associated kinase 3 antibody
    • Interleukin 1 receptor associated kinase M antibody
    • Interleukin-1 receptor-associated kinase 3 antibody
    • IRAK M antibody
    • IRAK-3 antibody
    • IRAK-M antibody
    • Irak3 antibody
    • IRAK3_HUMAN antibody
    see all

Images

  • All lanes : Anti-IRAKM antibody - C-terminal (ab189128) at 1 µg/ml

    Lane 1 : Mouse spleen lysate
    Lane 2 : Rat liver lysate

    Predicted band size: 68 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling IRAKM with ab189128 at 2 µg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

  • Immunofluorescent analysis of rat liver cells labeling IRAKM with ab189128 at 10 µg/ml.

References

This product has been referenced in:

  • Ge ZW  et al. IRAK3 gene silencing prevents cardiac rupture and ventricular remodeling through negative regulation of the NF-?B signaling pathway in a mouse model of acute myocardial infarction. J Cell Physiol N/A:N/A (2018). Read more (PubMed: 30536946) »
See 1 Publication for this product

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