Product nameIRAKM overexpression 293T lysate (whole cell)
ab94127 is a 293T cell transfected lysate in which Human IRAKM has been transiently over-expressed using a pCMV-IRAKM plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 6.80
Constituent: 100% 1x Sample Buffer
Concentration information loading...
BackgroundFunction: Inhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex. Tissue specificity: Expressed predominantly in peripheral blood lymphocytes. Disease: Defects in IRAK3 are associated with susceptibility to asthma-related traits type 5 (ASRT5) [MIM:611064]. Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing, dyspnea, bronchial hyperresponsiveness as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis. Similarity: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily. Contains 1 death domain. Contains 1 protein kinase domain.
Our Abpromise guarantee covers the use of ab94127 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94127 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-IRAKM antibody (ab56880) at 1/500 dilution
Lane 1 :
IRAKM overexpression 293T lysate (whole cell) (ab94127)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94127 has not yet been referenced specifically in any publications.