Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13409] to IRBIT
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Human
Product nameAnti-IRBIT antibody [EPR13409]
See all IRBIT primary antibodies
DescriptionRabbit monoclonal [EPR13409] to IRBIT
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human IRBIT aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: O43865
- Human thymus, HeLa, Ramos and 293T lysates; HeLa cells; 293T cells.
Protein previously labeled as AHCYL1.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab178693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).|
|ICC/IF||1/250 - 1/500.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Tissue specificityExpressed in dendritic cells.
PathwayAmino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.
Sequence similaritiesBelongs to the adenosylhomocysteinase family.
Developmental stageExpression increases markedly during activation of blood and skin DC (Langerhans cells), but is diminished in terminally differentiated tonsil DC.
DomainThe PEST region is essential for the interaction with ITPR1. The PDZ-binding region is required for maximal interaction with ITPR1 and is also responsible for the IP3-insensitive interaction with ITPR1.
Cellular localizationEndoplasmic reticulum.
- Information by UniProt
- AA409031 antibody
- AdoHcyase 2 antibody
- Ahcy-rs3 antibody
All lanes : Anti-IRBIT antibody [EPR13409] (ab178693) at 1/10000 dilution
Lane 1 : Human thymus lysate
Lane 2 : HeLa lysate
Lane 3 : Ramos lysate
Lane 4 : 293T lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 59 kDa
Immunofluorescent analysis of HeLa cells labeling IRBIT with ab178693 at 1/250 (red) and DAPI staining (blue).
Flow Cytometrical analysis of permeabilized 293T cells labeling IRBIT with ab178693 at 1/10 (red) or a rabbit IgG (negative) (green).
ab178693 has been referenced in 2 publications.
- García-Berrocoso T et al. Single Cell Immuno-Laser Microdissection Coupled to Label-Free Proteomics to Reveal the Proteotypes of Human Brain Cells After Ischemia. Mol Cell Proteomics 17:175-189 (2018). PubMed: 29133510
- Kruse M et al. Dynamics of Phosphoinositide-Dependent Signaling in Sympathetic Neurons. J Neurosci 36:1386-400 (2016). PubMed: 26818524