Overview

  • Product name

    Anti-IRBIT antibody [EPR13409]
    See all IRBIT primary antibodies
  • Description

    Rabbit monoclonal [EPR13409] to IRBIT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human IRBIT aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: O43865

  • Positive control

    • Human thymus, HeLa, Ramos and 293T lysates; HeLa cells; 293T cells.
  • General notes

    Protein previously labeled as AHCYL1.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab178693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
ICC/IF 1/250 - 1/500.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Tissue specificity

      Expressed in dendritic cells.
    • Pathway

      Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.
    • Sequence similarities

      Belongs to the adenosylhomocysteinase family.
    • Developmental stage

      Expression increases markedly during activation of blood and skin DC (Langerhans cells), but is diminished in terminally differentiated tonsil DC.
    • Domain

      The PEST region is essential for the interaction with ITPR1. The PDZ-binding region is required for maximal interaction with ITPR1 and is also responsible for the IP3-insensitive interaction with ITPR1.
    • Cellular localization

      Endoplasmic reticulum.
    • Information by UniProt
    • Database links

    • Alternative names

      • AA409031 antibody
      • AdoHcyase 2 antibody
      • Ahcy-rs3 antibody
      • AHCYL1 antibody
      • DC expressed AHCY like molecule antibody
      • DC-expressed AHCY-like molecule antibody
      • DCAL antibody
      • Dendritic cell expressed AHCY like protein antibody
      • Inositol 1,4,5-trisphosphate receptor-binding protein antibody
      • IP3R binding protein released with inositol 1,4,5-trisphosphate antibody
      • IRBIT antibody
      • PRO0233 antibody
      • Putative adenosylhomocysteinase 2 antibody
      • S adenosyl L homocysteine hydrolase 2 antibody
      • S adenosylhomocysteine hydrolase like 1 antibody
      • S-adenosyl-L-homocysteine hydrolase 2 antibody
      • S-adenosylhomocysteine hydrolase-like protein 1 antibody
      • SAHH2_HUMAN antibody
      • XPVKONA antibody
      see all

    Images

    • All lanes : Anti-IRBIT antibody [EPR13409] (ab178693) at 1/10000 dilution

      Lane 1 : Human thymus lysate
      Lane 2 : HeLa lysate
      Lane 3 : Ramos lysate
      Lane 4 : 293T lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 59 kDa

    • Immunofluorescent analysis of HeLa cells labeling IRBIT with ab178693 at 1/250 (red) and DAPI staining (blue).

    • Flow Cytometrical analysis of permeabilized 293T cells labeling IRBIT with ab178693 at 1/10 (red) or a rabbit IgG (negative) (green).

    References

    This product has been referenced in:

    • García-Berrocoso T  et al. Single Cell Immuno-Laser Microdissection Coupled to Label-Free Proteomics to Reveal the Proteotypes of Human Brain Cells After Ischemia. Mol Cell Proteomics 17:175-189 (2018). Read more (PubMed: 29133510) »
    • Kruse M  et al. Dynamics of Phosphoinositide-Dependent Signaling in Sympathetic Neurons. J Neurosci 36:1386-400 (2016). Read more (PubMed: 26818524) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab178693.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up