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    ire1-antibody-9f2-ab96481.pdf

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Signal Transduction Protein Phosphorylation Ser / Thr Kinases Other Kinases
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Anti-IRE1 antibody [9F2] (ab96481)

  • Datasheet
  • SDS
Reviews (4)Q&A (1)References (5)

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Western blot - Anti-IRE1 antibody [9F2] (ab96481)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRE1 antibody [9F2] (ab96481)
  • Flow Cytometry - Anti-IRE1 antibody [9F2] (ab96481)

Key features and details

  • Mouse monoclonal [9F2] to IRE1
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-IRE1 antibody [9F2]
    See all IRE1 primary antibodies
  • Description

    Mouse monoclonal [9F2] to IRE1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Purified recombinant fragment of Human IRE1 (aa282-433) expressed in E.coli.

  • Positive control

    • Raji, A431, Jurkat, Hela and HEK293 cell lysate; Human brain and stomach tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    9F2
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human IRE1 protein (ab114174)

Applications

Our Abpromise guarantee covers the use of ab96481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 110 kDa.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/100 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
  • Tissue specificity

    Ubiquitously expressed. High levels observed in pancreatic tissue.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 KEN domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated.
  • Cellular localization

    Endoplasmic reticulum membrane.
  • Target information above from: UniProt accession O75460 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10595 Human
    • Entrez Gene: 2081 Human
    • Omim: 604033 Human
    • SwissProt: O75460 Human
    • SwissProt: Q76MJ5 Human
    • Unigene: 133982 Human
    • Unigene: 592041 Human
    • Unigene: 700027 Human
    • Alternative names

      • Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
      • Endoplasmic reticulum to nucleus signaling 1 antibody
      • Endoplasmic reticulum-to-nucleus signaling 1 antibody
      • Endoribonuclease antibody
      • ER to nucleus signaling 1 antibody
      • ERN 1 antibody
      • Ern1 antibody
      • ERN1_HUMAN antibody
      • hIRE 1p antibody
      • hIRE1p antibody
      • Inositol requiring 1 antibody
      • Inositol requiring 1, S. cerevisiae, homolog of antibody
      • Inositol requiring enzyme 1, S. cerevisiae, homolog of antibody
      • Inositol requiring protein 1 antibody
      • inositol-requiring enzyme 1 antibody
      • Inositol-requiring protein 1 antibody
      • IRE 1 antibody
      • IRE 1a antibody
      • IRE 1P antibody
      • Ire1 alpha antibody
      • Ire1-alpha antibody
      • IRE1a antibody
      • Ire1alpha antibody
      • IRE1P antibody
      • MGC163277 antibody
      • MGC163279 antibody
      • Protein kinase/endoribonuclease antibody
      • RGD1559716 antibody
      • Serine/threonine protein kinase/endoribonuclease IRE1 antibody
      see all

    Images

    • Western blot - Anti-IRE1 antibody [9F2] (ab96481)
      Western blot - Anti-IRE1 antibody [9F2] (ab96481)
      All lanes : Anti-IRE1 antibody [9F2] (ab96481) at 1/500 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : HeLa cell lysate
      Lane 5 : HEK293 cell lysate

      Predicted band size: 110 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRE1 antibody [9F2] (ab96481)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRE1 antibody [9F2] (ab96481)
      Immunohistochemical analysis of paraffin-embedded Human brain tissue (A) and stomach tissue (B), showing cytoplasmic localization using ab96481 at 1/200 dilution with DAB staining.
    • Flow Cytometry - Anti-IRE1 antibody [9F2] (ab96481)
      Flow Cytometry - Anti-IRE1 antibody [9F2] (ab96481)
      Overlay histogram showing Raji cells stained with ab96481 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab96481, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Raji cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (5)

    Publishing research using ab96481? Please let us know so that we can cite the reference in this datasheet.

    ab96481 has been referenced in 5 publications.

    • Li K  et al. Deficiency of the IRE1a-Autophagy Axis Enhances the Antitumor Effects of the Oncolytic Virus M1. J Virol 92:N/A (2018). PubMed: 29263275
    • Jeon YJ  et al. miRNA-mediated TUSC3 deficiency enhances UPR and ERAD to promote metastatic potential of NSCLC. Nat Commun 9:5110 (2018). PubMed: 30504895
    • Ohana J  et al. dTCApFs, a derivative of a novel human hormone peptide, induces apoptosis in cancer cells through a mechanism involving loss of Golgi function. Mol Clin Oncol 7:991-999 (2017). PubMed: 29285362
    • Wang L  et al. Gemcitabine treatment induces endoplasmic reticular (ER) stress and subsequently upregulates urokinase plasminogen activator (uPA) to block mitochondrial-dependent apoptosis in Panc-1 cancer stem-like cells (CSCs). PLoS One 12:e0184110 (2017). PubMed: 28854261
    • Tamura K  et al. SHISA2 enhances the aggressive phenotype in prostate cancer through the regulation of WNT5A expression. Oncol Lett 14:6650-6658 (2017). PubMed: 29344118

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-5 of 5 Abreviews or Q&A

    Western blot abreview for Anti-IRE1 antibody [9F2]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (heart)
    Gel Running Conditions
    Non-reduced Denaturing (10%)
    Loading amount
    20 µg
    Specification
    heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 22 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-IRE1 antibody [9F2]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Skov3 (ovary cancer cells))
    Permeabilization
    Yes - 0.25% Tritox X-100
    Specification
    Skov3 (ovary cancer cells)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 07 2015

    ELISA abreview for Anti-IRE1 antibody [9F2]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Human Serum (926 Cytoplam/Nuclear extracts)
    Specification
    926 Cytoplam/Nuclear extracts
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Type
    Direct
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 11 2015

    Western blot abreview for Anti-IRE1 antibody [9F2]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (glioma)
    Loading amount
    20 µg
    Specification
    glioma
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 27 2012

    Question

    Dear abcam technical support team: This customer would like to order ab96481, ab79483 and ab25898, he would likes to order these antibodies and test in WB with rat sample, would you please help this customer to create a discount code, the information of this customer as follow: Name: Email: Institution: Could you please help this customer to solve these problems? Thanks for your kindly help.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 07 2011

    Answer

    DISCOUNT CODES: ab96481 : ******** ab79483 : ******** ab25898 : ******** Expiration date: DD MM YYYY I am very pleased to hear you would like to accept our offer and test these antibodies in rat. This code will give you 1 free primary for each antibody tested before the expiration date. To redeem this offer, please submit an Abreview for rat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Dec 07 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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