Product nameAnti-IRE1 antibody [9F2]
See all IRE1 primary antibodies
DescriptionMouse monoclonal [9F2] to IRE1
Tested applicationsSuitable for: WB, ELISA, IHC-P, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Human
Purified recombinant fragment of Human IRE1 (aa282-433) expressed in E.coli.
- Raji, A431, Jurkat, Hela and HEK293 cell lysate; Human brain and stomach tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab96481 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 110 kDa.|
|IHC-P||1/200 - 1/1000.|
|Flow Cyt||1/100 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/200 - 1/1000.|
FunctionSenses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
Tissue specificityUbiquitously expressed. High levels observed in pancreatic tissue.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 KEN domain.
Contains 1 protein kinase domain.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
- Endoplasmic reticulum to nucleus signaling 1 antibody
- Endoplasmic reticulum-to-nucleus signaling 1 antibody
All lanes : Anti-IRE1 antibody [9F2] (ab96481) at 1/500 dilution
Lane 1 : Raji cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : HEK293 cell lysate
Predicted band size: 110 kDa
Immunohistochemical analysis of paraffin-embedded Human brain tissue (A) and stomach tissue (B), showing cytoplasmic localization using ab96481 at 1/200 dilution with DAB staining.
Overlay histogram showing Raji cells stained with ab96481 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab96481, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Raji cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This product has been referenced in:
- Li K et al. Deficiency of the IRE1a-Autophagy Axis Enhances the Antitumor Effects of the Oncolytic Virus M1. J Virol 92:N/A (2018). Read more (PubMed: 29263275) »
- Jeon YJ et al. miRNA-mediated TUSC3 deficiency enhances UPR and ERAD to promote metastatic potential of NSCLC. Nat Commun 9:5110 (2018). Read more (PubMed: 30504895) »