Overview

  • Product name

  • Description

    Rabbit polyclonal to IRE1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide consisting of 16 amino acid from near the carboxy terminus (Human).

  • Positive control

    • WB: A20 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab37073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 107 kDa (predicted molecular weight: 110 kDa).

Please note there is a smaller isoform of IRE1 in rat (predicted molecular weight:68.6kDa; accession number is EDM06401). Block for 2 hours at RT or overnight at 4C using 5% non-fat milk.

ICC/IF Use a concentration of 1 µg/ml.
ICC Use a concentration of 1 µg/ml.

Target

  • Function

    Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
  • Tissue specificity

    Ubiquitously expressed. High levels observed in pancreatic tissue.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 KEN domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated.
  • Cellular localization

    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
    • Endoplasmic reticulum to nucleus signaling 1 antibody
    • Endoplasmic reticulum-to-nucleus signaling 1 antibody
    • Endoribonuclease antibody
    • ER to nucleus signaling 1 antibody
    • ERN 1 antibody
    • Ern1 antibody
    • ERN1_HUMAN antibody
    • hIRE 1p antibody
    • hIRE1p antibody
    • Inositol requiring 1 antibody
    • Inositol requiring 1, S. cerevisiae, homolog of antibody
    • Inositol requiring enzyme 1, S. cerevisiae, homolog of antibody
    • Inositol requiring protein 1 antibody
    • inositol-requiring enzyme 1 antibody
    • Inositol-requiring protein 1 antibody
    • IRE 1 antibody
    • IRE 1a antibody
    • IRE 1P antibody
    • Ire1 alpha antibody
    • Ire1-alpha antibody
    • IRE1a antibody
    • Ire1alpha antibody
    • IRE1P antibody
    • MGC163277 antibody
    • MGC163279 antibody
    • Protein kinase/endoribonuclease antibody
    • RGD1559716 antibody
    • Serine/threonine protein kinase/endoribonuclease IRE1 antibody
    see all

Images

  • Western Blot of rat brain lysate labeling IRE1 with Anti-IRE1 antibody (ab37073) at (1) 0.5 and (2) 1µg/ml.
  • Lane 1 : Anti-IRE1 antibody (ab37073) at 0.5 µg/ml
    Lane 2 : Anti-IRE1 antibody (ab37073) at 1 µg/ml
    Lane 3 : Anti-IRE1 antibody (ab37073) at 2 µg/ml

    All lanes : A20 cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/10000 dilution

    Predicted band size: 110 kDa
    Observed band size: 107 kDa
    why is the actual band size different from the predicted?

  • ab37073, at 1 µg/ml, staining IRE1 in A20 cells with by ICC.

  • Immunocytochemistry/Immunofluorescence of IRE1p in A20 cells using ab37073 at 2 ug/ml.

References

This product has been referenced in:

  • Trotta MC  et al. The activation of retinal HCA2 receptors by systemic beta-hydroxybutyrate inhibits diabetic retinal damage through reduction of endoplasmic reticulum stress and the NLRP3 inflammasome. PLoS One 14:e0211005 (2019). Read more (PubMed: 30657794) »
  • Pandey VK  et al. Activation of PERK-eIF2a-ATF4 pathway contributes to diabetic hepatotoxicity: Attenuation of ER stress by Morin. Cell Signal 59:41-52 (2019). Read more (PubMed: 30877037) »
See all 58 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (heart)
Gel Running Conditions
Non-reduced Denaturing (10%)
Loading amount
20 µg
Specification
heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Aug 21 2018

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I will let you know as soon as I have any further information regarding the immunogen sequences for these antibodies.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1145331.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More
Application
Western blot
Sample
Mouse Cell lysate - whole cell (CEC)
Loading amount
30 µg
Specification
CEC
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 22 2008

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