Anti-IRE1 antibody (ab37117)
Key features and details
- Rabbit polyclonal to IRE1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-IRE1 antibody
See all IRE1 primary antibodies -
Description
Rabbit polyclonal to IRE1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide corresponding to 15 amino acids near the N terminus of the human IRE1 protein.
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Positive control
- A20 cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab37117 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 1 µg/ml.
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WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 107 kDa (predicted molecular weight: 110 kDa).
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Notes |
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ICC/IF
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 107 kDa (predicted molecular weight: 110 kDa). |
Target
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Function
Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. -
Tissue specificity
Ubiquitously expressed. High levels observed in pancreatic tissue. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 KEN domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 78943 Mouse
- SwissProt: Q9EQY0 Mouse
- SwissProt: Q9Z2E3 Mouse
- Unigene: 20452 Mouse
- Unigene: 340943 Mouse
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Alternative names
- Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
- Endoplasmic reticulum to nucleus signaling 1 antibody
- Endoplasmic reticulum-to-nucleus signaling 1 antibody
see all
Images
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All lanes : Anti-IRE1 antibody (ab37117)
Lane 1 : ab37117 at 0.5µg/ml + A20 cell lysate
Lane 2 : ab37117 at 1µg/ml + A20 Cell lysate
Lane 3 : ab37117 at 2µg/ml + A20 cell lysate
Predicted band size: 110 kDa
Observed band size: 107 kDa why is the actual band size different from the predicted?
Additional bands at: 150 kDa, 55 kDa. We are unsure as to the identity of these extra bands. -
Immunofluorescence of IRE1p in A20 cells using ab37117 at 10 ug/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab37117 has been referenced in 3 publications.
- Wang C et al. Interaction of Estradiol and Endoplasmic Reticulum Stress in the Development of Esophageal Carcinoma. Front Endocrinol (Lausanne) 11:410 (2020). PubMed: 32793111
- Yin M et al. Stellate Ganglion Blockade repairs Intestinal Mucosal Barrier through suppression of Endoplasmic Reticulum Stress following Hemorrhagic Shock. Int J Med Sci 17:2147-2154 (2020). PubMed: 32922175
- Yuan F et al. Gastrodin Ameliorates Acute Rejection via IRE1a/TRAF2/NF-?B in Rats Receiving Liver Allografts. Biomed Res Int 2019:9276831 (2019). PubMed: 31828147