Key features and details
- Rabbit polyclonal to IRE1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-IRE1 antibody
See all IRE1 primary antibodies
DescriptionRabbit polyclonal to IRE1
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to 15 amino acids near the N terminus of the human IRE1 protein.
- A20 cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab37117 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 107 kDa (predicted molecular weight: 110 kDa).|
FunctionSenses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
Tissue specificityUbiquitously expressed. High levels observed in pancreatic tissue.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 KEN domain.
Contains 1 protein kinase domain.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
- Endoplasmic reticulum to nucleus signaling 1 antibody
- Endoplasmic reticulum-to-nucleus signaling 1 antibody
All lanes : Anti-IRE1 antibody (ab37117)
Lane 1 : ab37117 at 0.5µg/ml + A20 cell lysate
Lane 2 : ab37117 at 1µg/ml + A20 Cell lysate
Lane 3 : ab37117 at 2µg/ml + A20 cell lysate
Predicted band size: 110 kDa
Observed band size: 107 kDa why is the actual band size different from the predicted?
Additional bands at: 150 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Immunofluorescence of IRE1p in A20 cells using ab37117 at 10 ug/ml.
ab37117 has been referenced in 1 publication.
- Yuan F et al. Gastrodin Ameliorates Acute Rejection via IRE1a/TRAF2/NF-?B in Rats Receiving Liver Allografts. Biomed Res Int 2019:9276831 (2019). PubMed: 31828147