Overview

  • Product name

    Anti-IRE1 antibody - N-terminal
    See all IRE1 primary antibodies
  • Description

    Rabbit polyclonal to IRE1 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human IRE1 (N terminal). The exact sequence is proprietary.
    Database link: O75460

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 110 kDa.

Target

  • Function

    Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
  • Tissue specificity

    Ubiquitously expressed. High levels observed in pancreatic tissue.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 KEN domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated.
  • Cellular localization

    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
    • Endoplasmic reticulum to nucleus signaling 1 antibody
    • Endoplasmic reticulum-to-nucleus signaling 1 antibody
    • Endoribonuclease antibody
    • ER to nucleus signaling 1 antibody
    • ERN 1 antibody
    • Ern1 antibody
    • ERN1_HUMAN antibody
    • hIRE 1p antibody
    • hIRE1p antibody
    • Inositol requiring 1 antibody
    • Inositol requiring 1, S. cerevisiae, homolog of antibody
    • Inositol requiring enzyme 1, S. cerevisiae, homolog of antibody
    • Inositol requiring protein 1 antibody
    • inositol-requiring enzyme 1 antibody
    • Inositol-requiring protein 1 antibody
    • IRE 1 antibody
    • IRE 1a antibody
    • IRE 1P antibody
    • Ire1 alpha antibody
    • Ire1-alpha antibody
    • IRE1a antibody
    • Ire1alpha antibody
    • IRE1P antibody
    • MGC163277 antibody
    • MGC163279 antibody
    • Protein kinase/endoribonuclease antibody
    • RGD1559716 antibody
    • Serine/threonine protein kinase/endoribonuclease IRE1 antibody
    see all

Images

  • All lanes : Anti-IRE1 antibody - N-terminal (ab227245) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 110 kDa



    7.5% SDS-PAGE gel.

References

ab227245 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227245.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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