Product nameAnti-IRE1 (phospho S724) antibody
See all IRE1 primary antibodies
DescriptionRabbit polyclonal to IRE1 (phospho S724)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Medaka fish
Synthetic peptide within Human IRE1 (internal sequence) (phospho S724). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: O75460
- WB: HepG2, treated with 3 µM Thapsigargin for 12 hours, whole cell extract.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 110 kDa.|
FunctionSenses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
Tissue specificityUbiquitously expressed. High levels observed in pancreatic tissue.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 KEN domain.
Contains 1 protein kinase domain.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
- Endoplasmic reticulum to nucleus signaling 1 antibody
- Endoplasmic reticulum-to-nucleus signaling 1 antibody
All lanes : Anti-IRE1 (phospho S724) antibody (ab226974) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) treated with 3 µM Thapsigargin for 12 hours, whole cell extract
Lysates/proteins at 20 µg per lane.
Predicted band size: 110 kDa
7.5% SDS-PAGE gel.
ab226974 has not yet been referenced specifically in any publications.