Key features and details
- Rabbit polyclonal to IRE1 (phospho S724)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IRE1 (phospho S724) antibody
See all IRE1 primary antibodies
DescriptionRabbit polyclonal to IRE1 (phospho S724)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Medaka fish
Synthetic peptide within Human IRE1 (internal sequence) (phospho S724). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: O75460
- WB: HepG2, treated with 3 µM Thapsigargin for 12 hours, whole cell extract.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 110 kDa.|
FunctionSenses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
Tissue specificityUbiquitously expressed. High levels observed in pancreatic tissue.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 KEN domain.
Contains 1 protein kinase domain.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
- Endoplasmic reticulum to nucleus signaling 1 antibody
- Endoplasmic reticulum-to-nucleus signaling 1 antibody
All lanes : Anti-IRE1 (phospho S724) antibody (ab226974) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) treated with 3 µM Thapsigargin for 12 hours, whole cell extract
Lysates/proteins at 20 µg per lane.
Predicted band size: 110 kDa
7.5% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226974 has been referenced in 1 publication.
- Wang J et al. Microcystin-LR ameliorates pulmonary fibrosis via modulating CD206+ M2-like macrophage polarization. Cell Death Dis 11:136 (2020). PubMed: 32075954