Anti-IRE1 (phospho S724) antibody (Biotin) (ab81936)

Rabbit polyclonal IRE1 (phospho S724) antibody conjugated to Biotin. Validated in WB and tested in Mouse, Rat, Human. Cited in 1 publication(s). Independently reviewed in 1 review(s).

Overview

  • Product name

    Anti-IRE1 (phospho S724) antibody (Biotin)
    See all IRE1 primary antibodies
  • Description

    Rabbit polyclonal to IRE1 (phospho S724) (Biotin)
  • Host species

    Rabbit
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Does not react with: Monkey
  • Immunogen

    Synthetic peptide surrounding the phosphorylated serine 724 of human IRE1.

  • Positive control

    • Glucose treated pancreatic islets and endogenous protein of pancreatic beta cells and insuli. COS-7 expressing wild-type IRE1. Min6 cells treated with glucose.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituents: 30% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).

Target

  • Function

    Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
  • Tissue specificity

    Ubiquitously expressed. High levels observed in pancreatic tissue.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 KEN domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated.
  • Cellular localization

    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
    • Endoplasmic reticulum to nucleus signaling 1 antibody
    • Endoplasmic reticulum-to-nucleus signaling 1 antibody
    • Endoribonuclease antibody
    • ER to nucleus signaling 1 antibody
    • ERN 1 antibody
    • Ern1 antibody
    • ERN1_HUMAN antibody
    • hIRE 1p antibody
    • hIRE1p antibody
    • Inositol requiring 1 antibody
    • Inositol requiring 1, S. cerevisiae, homolog of antibody
    • Inositol requiring enzyme 1, S. cerevisiae, homolog of antibody
    • Inositol requiring protein 1 antibody
    • inositol-requiring enzyme 1 antibody
    • Inositol-requiring protein 1 antibody
    • IRE 1 antibody
    • IRE 1a antibody
    • IRE 1P antibody
    • Ire1 alpha antibody
    • Ire1-alpha antibody
    • IRE1a antibody
    • Ire1alpha antibody
    • IRE1P antibody
    • MGC163277 antibody
    • MGC163279 antibody
    • Protein kinase/endoribonuclease antibody
    • RGD1559716 antibody
    • Serine/threonine protein kinase/endoribonuclease IRE1 antibody
    see all

References

This product has been referenced in:

  • He Y  et al. Palmitic acid, but not high-glucose, induced myocardial apoptosis is alleviated by N-acetylcysteine due to attenuated mitochondrial-derived ROS accumulation-induced endoplasmic reticulum stress. Cell Death Dis 9:568 (2018). Read more (PubMed: 29752433) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (AR42J)
Permeabilization
Yes - 10% Triton x100
Specification
AR42J
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 24 2017

Question
Answer

Thank you very much for your call today.
DISCOUNT CODE: ***
Expiration date: December 14, 2012
This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab104157 with rat samples and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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