Biotin Anti-IRE1 (phospho S724) antibody (ab81936)
Key features and details
- Biotin Rabbit polyclonal to IRE1 (phospho S724)
- Suitable for: IHC-P
- Reacts with: Mouse, Rat, Human
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-IRE1 (phospho S724) antibody
See all IRE1 primary antibodies -
Description
Biotin Rabbit polyclonal to IRE1 (phospho S724) -
Host species
Rabbit -
Conjugation
Biotin -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Does not react with: Monkey -
Immunogen
Synthetic peptide corresponding to Human IRE1 (phospho S724).
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Positive control
- Glucose treated pancreatic islets and endogenous protein of pancreatic beta cells and insuli. COS-7 expressing wild-type IRE1. Min6 cells treated with glucose.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 30% Glycerol, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab81936 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/300.
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Notes |
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IHC-P
1/300. |
Target
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Function
Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. -
Tissue specificity
Ubiquitously expressed. High levels observed in pancreatic tissue. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 KEN domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 10595 Human
- Entrez Gene: 2081 Human
- Entrez Gene: 78943 Mouse
- Entrez Gene: 498013 Rat
- Omim: 604033 Human
- SwissProt: O75460 Human
- SwissProt: Q76MJ5 Human
- SwissProt: Q9EQY0 Mouse
see all -
Alternative names
- Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
- Endoplasmic reticulum to nucleus signaling 1 antibody
- Endoplasmic reticulum-to-nucleus signaling 1 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab81936 has been referenced in 1 publication.
- He Y et al. Palmitic acid, but not high-glucose, induced myocardial apoptosis is alleviated by N-acetylcysteine due to attenuated mitochondrial-derived ROS accumulation-induced endoplasmic reticulum stress. Cell Death Dis 9:568 (2018). PubMed: 29752433