Anti-IREB2/IRP2 antibody (ab106926)
Key features and details
- Goat polyclonal to IREB2/IRP2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-IREB2/IRP2 antibody
See all IREB2/IRP2 primary antibodies -
Description
Goat polyclonal to IREB2/IRP2 -
Host species
Goat -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Orangutan -
Immunogen
Synthetic peptide corresponding to Human IREB2/IRP2 aa 489-503 (internal sequence).
Sequence:C-SIHYEGSEYKLSHGS
Database link: NP_004127.1 -
Positive control
- WB: Human Liver lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab106926 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106926 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 3 - 6 µg/ml.
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WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 105 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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IHC-P
Use a concentration of 3 - 6 µg/ml. |
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 105 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA. -
Sequence similarities
Belongs to the aconitase/IPM isomerase family. -
Post-translational
modificationsUbiquitinated and degraded by the proteasome in presence of high level of iron and oxygen. Ubiquitinated by a SCF complex containing FBXL5. Upon iron and oxygen depletion FBXL5 is degraded, preventing ubiquitination and allowing its RNA-binding activity. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 539404 Cow
- Entrez Gene: 3658 Human
- Entrez Gene: 64602 Mouse
- Entrez Gene: 100174315 Orangutan
- Entrez Gene: 100153993 Pig
- Entrez Gene: 64831 Rat
- Omim: 147582 Human
- SwissProt: P48200 Human
see all -
Alternative names
- ACO3 antibody
- FLJ23381 antibody
- IRE BP2 antibody
see all
Images
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All lanes : Anti-IREB2/IRP2 antibody (ab106926) at 0.3 µg/ml
Lane 1 : Human Liver lysate in RIPA buffer
Lane 2 : Human Liver lysate in RIPA buffer with immunizing peptide
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 105 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
Primary incubation was 1 hour.
An additional band of unknown identity was consistently observed at 60kDa. This band was successfully blocked by incubation with the immunizing peptide.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab106926 has been referenced in 1 publication.
- Cloonan SM et al. Mitochondrial iron chelation ameliorates cigarette smoke-induced bronchitis and emphysema in mice. Nat Med 22:163-74 (2016). PubMed: 26752519