Key features and details
- Goat polyclonal to IREB2/IRP2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IREB2/IRP2 antibody
See all IREB2/IRP2 primary antibodies
DescriptionGoat polyclonal to IREB2/IRP2
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Orangutan
- Human Liver lysate Human kidney
This product was previously labelled as IREB2
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab106926 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab106926 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 3 - 6 µg/ml.|
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 105 kDa).
1 hour primary incubation is recommended for this product.
FunctionRNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA.
Sequence similaritiesBelongs to the aconitase/IPM isomerase family.
modificationsUbiquitinated and degraded by the proteasome in presence of high level of iron and oxygen. Ubiquitinated by a SCF complex containing FBXL5. Upon iron and oxygen depletion FBXL5 is degraded, preventing ubiquitination and allowing its RNA-binding activity.
- Information by UniProt
- ACO3 antibody
- FLJ23381 antibody
- IRE BP2 antibody
All lanes : Anti-IREB2/IRP2 antibody (ab106926) at 0.3 µg/ml
Lane 1 : Human Liver lysate in RIPA buffer
Lane 2 : Human Liver lysate in RIPA buffer with immunizing peptide
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 105 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
Primary incubation was 1 hour.
An additional band of unknown identity was consistently observed at 60kDa. This band was successfully blocked by incubation with the immunizing peptide.
ab106926 at 3.8 µg/ml staining IREB2/IRP2 in Human spleen by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed. Antibody was revealed with AP-staining.
ab106926 has been referenced in 1 publication.
- Cloonan SM et al. Mitochondrial iron chelation ameliorates cigarette smoke-induced bronchitis and emphysema in mice. Nat Med 22:163-74 (2016). PubMed: 26752519