Anti-IREB2/IRP2 antibody (ab232994)
Key features and details
- Rabbit polyclonal to IREB2/IRP2
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Overview
-
Product name
Anti-IREB2/IRP2 antibody
See all IREB2/IRP2 primary antibodies -
Description
Rabbit polyclonal to IREB2/IRP2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human IREB2/IRP2 aa 1-300. Expressed in E.coli.
Database link: P48200 -
Positive control
- WB: Recombinant human IREB2/IRP2 protein; Pig liver lysate; Human lung tissue lysate; HeLa and A549 whole cell lysates. IHC-P: Human kidney, skin cancer and lung cancer tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 105 kDa.
|
|
IHC-P |
Use a concentration of 5 - 20 µg/ml.
|
Notes |
---|
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 105 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
-
Function
RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA. -
Sequence similarities
Belongs to the aconitase/IPM isomerase family. -
Post-translational
modificationsUbiquitinated and degraded by the proteasome in presence of high level of iron and oxygen. Ubiquitinated by a SCF complex containing FBXL5. Upon iron and oxygen depletion FBXL5 is degraded, preventing ubiquitination and allowing its RNA-binding activity. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 3658 Human
- Entrez Gene: 64602 Mouse
- Entrez Gene: 100153993 Pig
- Entrez Gene: 64831 Rat
- Omim: 147582 Human
- SwissProt: P48200 Human
- SwissProt: Q811J3 Mouse
- SwissProt: B3VKQ2 Pig
see all -
Alternative names
- ACO3 antibody
- FLJ23381 antibody
- IRE BP2 antibody
see all
Images
-
Formalin-fixed, paraffin-embedded human kidney tissue stained for IREB2/IRP2 using ab232994 at 10μg/ml in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human skin cancer tissue stained for IREB2/IRP2 using ab232994 at 10μg/ml in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human lung cancer tissue stained for IREB2/IRP2 using ab232994 at 10μg/ml in immunohistochemical analysis.
-
Anti-IREB2/IRP2 antibody (ab232994) at 3 µg/ml + Recombinant human IREB2/IRP2
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 105 kDa -
All lanes : Anti-IREB2/IRP2 antibody (ab232994) at 3 µg/ml
Lane 1 : Pig liver tissue lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : Human lung tissue lysate
Lane 4 : A549 (human lung carcinoma cell line) whole cell lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 105 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab232994 has been referenced in 3 publications.
- Zhang X et al. Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma. Theranostics 11:5650-5674 (2021). PubMed: 33897873
- Yao F et al. Iron regulatory protein 1 promotes ferroptosis by sustaining cellular iron homeostasis in melanoma. Oncol Lett 22:657 (2021). PubMed: 34386079
- Li Y et al. CISD3 inhibition drives cystine-deprivation induced ferroptosis. Cell Death Dis 12:839 (2021). PubMed: 34497268