Anti-IRF1 antibody (ab233087)
Key features and details
- Rabbit polyclonal to IRF1
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-IRF1 antibody
See all IRF1 primary antibodies -
Description
Rabbit polyclonal to IRF1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Pig -
Immunogen
Recombinant fragment (His-tag) corresponding to Human IRF1 aa 1-300. Expressed in E.coli. N-terminal tags.
Database link: P10914 -
Positive control
- WB: Rat heart and placenta lysates; Recombinant human IRF1 protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab233087 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233087 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 36 kDa.
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Notes |
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WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 36 kDa. |
Target
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Function
Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and activates those genes. Acts as a tumor suppressor. -
Involvement in disease
Defects in IRF1 are a cause of gastric cancer (GASC) [MIM:613659]; also called gastric cancer intestinal or stomach cancer. Gastric cancer is a malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions, resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease. -
Sequence similarities
Belongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain. -
Post-translational
modificationsSumoylation represses the transcriptional activity and displays enhanced resistance to protein degradation. Inactivates the tumor suppressor activity. Elevated levels in tumor cells. Major site is Lys-275. Sumoylation is enhanced by PIAS3 (By similarity). Desumoylated by SENP1 in tumor cells and appears to compete with ubiquitination on C-terminal sites.
Ubiquitinated. Appears to compete with sumoylation on C-terminal sites. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3659 Human
- Entrez Gene: 396611 Pig
- Entrez Gene: 24508 Rat
- Omim: 147575 Human
- SwissProt: P10914 Human
- SwissProt: A0FIN4 Pig
- SwissProt: P23570 Rat
- Unigene: 436061 Human
see all -
Alternative names
- Interferon regulatory factor 1 antibody
- Interferon regulatory factor 1 isoform +I9 antibody
- Interferon regulatory factor 1 isoform d78 antibody
see all
Images
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All lanes : Anti-IRF1 antibody (ab233087) at 2 µg/ml
Lane 1 : Rat heart lysate
Lane 2 : Rat placenta lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 36 kDa -
Anti-IRF1 antibody (ab233087) at 2 µg/ml + Recombinant human IRF1 protein
Predicted band size: 36 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab233087 has not yet been referenced specifically in any publications.