Anti-IRF2 antibody (ab245657)
Key features and details
- Rabbit polyclonal to IRF2
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-IRF2 antibody
See all IRF2 primary antibodies -
Description
Rabbit polyclonal to IRF2 -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide within Mouse IRF2 aa 100-150. The exact sequence is proprietary. NP_032417.3
Database link: P23906 -
Positive control
- IP: HEL whole cell lysate.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab245657 was affinity purified using an epitope specific to IRF2 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab245657 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP | Use at 2-10 µg/mg of lysate. |
Target
-
Function
Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and represses those genes. Also acts as an activator for several genes including H4 and IL7. Constitutively binds to the ISRE promoter to activate IL7. Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. Antagonizes IRF1 transcriptional activation. -
Tissue specificity
Expressed throughout the epithelium of the colon. Also expressed in lamina propria. -
Sequence similarities
Belongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain. -
Post-translational
modificationsAcetylated by CBP/ p300 during cell-growth. Acetylation on Lys-75 is required for stimulation of H4 promoter activity.
The major sites of sumoylation are Lys-137 and Lys-293. Sumoylation by SUMO1 increases its transcriptional repressor activity on IRF1 and diminishes its ability to activate ISRE and H4 promoter. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 3660 Human
- Entrez Gene: 16363 Mouse
- Omim: 147576 Human
- SwissProt: P14316 Human
- SwissProt: P23906 Mouse
- Unigene: 654566 Human
- Unigene: 1149 Mouse
-
Alternative names
- DKFZp686F0244 antibody
- Interferon regulatory factor 2 antibody
- IRF 2 antibody
see all
Images
-
IRF2 was immunoprecipitated from HEL (Human bone marrow erythroleukemia cell line) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).
ab245657 used for IP at 6 µg/mg lysate. For WB another anti-IRF2 antibody was used at 1 µg/ml.
Lane 1: ab245657 IP in HEL whole cell lysate.
Lane 2: Control IgG in HEL whole cell lysate.Chemiluminescence detection: 3 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab245657 has not yet been referenced specifically in any publications.