Anti-IRF2 antibody (ab245657)

Submit a review Submit a question

Overview

  • Product name

    Anti-IRF2 antibody
    See all IRF2 primary antibodies

  • Description

    Rabbit polyclonal to IRF2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide within Mouse IRF2 aa 100-150. The exact sequence is proprietary. NP_032417.3
    Database link: P23906

  • Positive control

    • IP: HEL whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab245657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.

    • Target

    • Function

      Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and represses those genes. Also acts as an activator for several genes including H4 and IL7. Constitutively binds to the ISRE promoter to activate IL7. Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. Antagonizes IRF1 transcriptional activation.

    • Tissue specificity

      Expressed throughout the epithelium of the colon. Also expressed in lamina propria.

    • Sequence similarities

      Belongs to the IRF family.
      Contains 1 IRF tryptophan pentad repeat DNA-binding domain.

    • Post-translational
      modifications

      Acetylated by CBP/ p300 during cell-growth. Acetylation on Lys-75 is required for stimulation of H4 promoter activity.
      The major sites of sumoylation are Lys-137 and Lys-293. Sumoylation by SUMO1 increases its transcriptional repressor activity on IRF1 and diminishes its ability to activate ISRE and H4 promoter.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • DKFZp686F0244 antibody
        • Interferon regulatory factor 2 antibody
        • IRF 2 antibody
        • IRF-2 antibody
        • IRF2 antibody
        • IRF2_HUMAN antibody
        see all

      Images

      • Immunoprecipitation - Anti-IRF2 antibody (ab245657)
        Immunoprecipitation - Anti-IRF2 antibody (ab245657)

        IRF2 was immunoprecipitated from HEL (Human bone marrow erythroleukemia cell line) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).

        ab245657 used for IP at 6 µg/mg lysate. For WB another anti-IRF2 antibody was used at 1 µg/ml.

        Lane 1: ab245657 IP in HEL whole cell lysate.
        Lane 2: Control IgG in HEL whole cell lysate.

        Chemiluminescence detection: 3 seconds.

      References

      ab245657 has not yet been referenced specifically in any publications.

      Publishing research using ab245657? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab245657.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com