Anti-IRF2 antibody (ab245657)

Submit a review Submit a question
Immunoprecipitation - Anti-IRF2 antibody (ab245657)

    Key features and details

    • Rabbit polyclonal to IRF2
    • Suitable for: IP
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-IRF2 antibody
      See all IRF2 primary antibodies

    • Description

      Rabbit polyclonal to IRF2

    • Host species

      Rabbit

    • Tested applications

      Suitable for: IPmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse

    • Immunogen

      Synthetic peptide within Mouse IRF2 aa 100-150. The exact sequence is proprietary. NP_032417.3
      Database link: P23906

    • Positive control

      • IP: HEL whole cell lysate.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab245657 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IP Use at 2-10 µg/mg of lysate.

    Target

    • Function

      Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and represses those genes. Also acts as an activator for several genes including H4 and IL7. Constitutively binds to the ISRE promoter to activate IL7. Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. Antagonizes IRF1 transcriptional activation.

    • Tissue specificity

      Expressed throughout the epithelium of the colon. Also expressed in lamina propria.

    • Sequence similarities

      Belongs to the IRF family.
      Contains 1 IRF tryptophan pentad repeat DNA-binding domain.

    • Post-translational
      modifications

      Acetylated by CBP/ p300 during cell-growth. Acetylation on Lys-75 is required for stimulation of H4 promoter activity.
      The major sites of sumoylation are Lys-137 and Lys-293. Sumoylation by SUMO1 increases its transcriptional repressor activity on IRF1 and diminishes its ability to activate ISRE and H4 promoter.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • DKFZp686F0244 antibody
        • Interferon regulatory factor 2 antibody
        • IRF 2 antibody
        • IRF-2 antibody
        • IRF2 antibody
        • IRF2_HUMAN antibody
        see all

      Images

      • Immunoprecipitation - Anti-IRF2 antibody (ab245657)
        Immunoprecipitation - Anti-IRF2 antibody (ab245657)

        IRF2 was immunoprecipitated from HEL (Human bone marrow erythroleukemia cell line) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).

        ab245657 used for IP at 6 µg/mg lysate. For WB another anti-IRF2 antibody was used at 1 µg/ml.

        Lane 1: ab245657 IP in HEL whole cell lysate.
        Lane 2: Control IgG in HEL whole cell lysate.

        Chemiluminescence detection: 3 seconds.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab245657? Please let us know so that we can cite the reference in this datasheet.

      ab245657 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab245657.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com