Key features and details
- Rabbit polyclonal to IRF2
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IRF2 antibody
See all IRF2 primary antibodies
DescriptionRabbit polyclonal to IRF2
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Mouse IRF2 aa 100-150. The exact sequence is proprietary. NP_032417.3
Database link: P23906
- IP: HEL whole cell lysate.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245657 was affinity purified using an epitope specific to IRF2 immobilized on solid support.
- Epigenetics and Nuclear Signaling
- Domain Families
- HLH / Leucine Zipper
- Epigenetics and Nuclear Signaling
- Polymerase associated factors
- Pol II Transcription
Our Abpromise guarantee covers the use of ab245657 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
FunctionSpecifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and represses those genes. Also acts as an activator for several genes including H4 and IL7. Constitutively binds to the ISRE promoter to activate IL7. Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. Antagonizes IRF1 transcriptional activation.
Tissue specificityExpressed throughout the epithelium of the colon. Also expressed in lamina propria.
Sequence similaritiesBelongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
modificationsAcetylated by CBP/ p300 during cell-growth. Acetylation on Lys-75 is required for stimulation of H4 promoter activity.
The major sites of sumoylation are Lys-137 and Lys-293. Sumoylation by SUMO1 increases its transcriptional repressor activity on IRF1 and diminishes its ability to activate ISRE and H4 promoter.
- Information by UniProt
- DKFZp686F0244 antibody
- Interferon regulatory factor 2 antibody
- IRF 2 antibody
IRF2 was immunoprecipitated from HEL (Human bone marrow erythroleukemia cell line) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).
ab245657 used for IP at 6 µg/mg lysate. For WB another anti-IRF2 antibody was used at 1 µg/ml.
Lane 1: ab245657 IP in HEL whole cell lysate.
Lane 2: Control IgG in HEL whole cell lysate.
Chemiluminescence detection: 3 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245657 has not yet been referenced specifically in any publications.