Key features and details
- Rabbit polyclonal to IRF3
- Suitable for: WB, IP
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-IRF3 antibody
See all IRF3 primary antibodies
DescriptionRabbit polyclonal to IRF3
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse
Synthetic peptide within Mouse IRF3 aa 375-419. The exact sequence is proprietary. NP_058545.1
Database link: P70671
- WB: Mouse bone marrow dendritic whole cell lysate; NIH/3T3 whole cell lysate. IP: Mouse bone marrow dendritic whole cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245341 was affinity purified using an epitope specific to IRF3 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245341 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2500. Predicted molecular weight: 47 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionMediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains.
Tissue specificityExpressed constitutively in a variety of tissues.
Sequence similaritiesBelongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
modificationsConstitutively phosphorylated on many serines residues. C-terminal serine/threonine cluster is phosphorylated in response of induction by IKBKE and TBK1. Ser-385 and Ser-386 may be specifically phosphorylated in response to induction. An alternate model propose that the five serine/threonine residues between 396 and 405 are phosphorylated in response to a viral infection. Phosphorylation, and subsequent activation of IRF3 is inhibited by vaccinia virus protein E3.
Ubiquitinated; ubiquitination involves RBCK1 leading to proteasomal degradation. Polyubiquitinated; ubiquitination involves TRIM21 leading to proteasomal degradation.
ISGylated by HERC5 resulting in sustained IRF3 activation and in the inhibition of IRF3 ubiquitination by disrupting PIN1 binding. The phosphorylation state of IRF3 does not alter ISGylation.
Cellular localizationCytoplasm. Nucleus. Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm.
- Information by UniProt
- IIAE7 antibody
- Interferon regulatory factor 3 antibody
- IRF 3 antibody
All lanes : Anti-IRF3 antibody (ab245341) at 0.4 µg/ml
Lane 1 : Mouse bone marrow dendritic whole cell lysate at 50 µg
Lane 2 : Mouse bone marrow dendritic whole cell lysate at 15 µg
Lane 3 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Predicted band size: 47 kDa
Exposure time: 3 minutes
IRF was immunoprecipitated from mouse bone marrow dendritic whole cell lysate (1 mg per IP reaction; 20% of IP loaded).
ab245341 used for IP at 6 µg/mg lysate. For WB 1 µg/ml.
Lane 1: ab245341 IP in mouse bone marrow dendritic whole cell lysate.
Lane 2: Control IgG IP in mouse bone marrow dendritic whole cell lysate.
Chemiluminescence detection: 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245341 has not yet been referenced specifically in any publications.