Anti-IRF3 antibody (ab25950)
Key features and details
- Rabbit polyclonal to IRF3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IRF3 antibody
See all IRF3 primary antibodies -
Description
Rabbit polyclonal to IRF3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
A synthetic peptide corresponding to 14 amino acids near the carboxyterminus of IRF3 (Human)
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Positive control
- WB: Ramos and HT-29 cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
IRF3 Antibody is affinity chromatography purified via peptide column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25950 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 47 kDa).
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 47 kDa). |
Target
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Function
Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains. -
Tissue specificity
Expressed constitutively in a variety of tissues. -
Sequence similarities
Belongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain. -
Post-translational
modificationsConstitutively phosphorylated on many serines residues. C-terminal serine/threonine cluster is phosphorylated in response of induction by IKBKE and TBK1. Ser-385 and Ser-386 may be specifically phosphorylated in response to induction. An alternate model propose that the five serine/threonine residues between 396 and 405 are phosphorylated in response to a viral infection. Phosphorylation, and subsequent activation of IRF3 is inhibited by vaccinia virus protein E3.
Ubiquitinated; ubiquitination involves RBCK1 leading to proteasomal degradation. Polyubiquitinated; ubiquitination involves TRIM21 leading to proteasomal degradation.
ISGylated by HERC5 resulting in sustained IRF3 activation and in the inhibition of IRF3 ubiquitination by disrupting PIN1 binding. The phosphorylation state of IRF3 does not alter ISGylation. -
Cellular localization
Cytoplasm. Nucleus. Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 3661 Human
- Omim: 603734 Human
- SwissProt: Q14653 Human
- Unigene: 289052 Human
- Unigene: 75254 Human
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Alternative names
- IIAE7 antibody
- Interferon regulatory factor 3 antibody
- IRF 3 antibody
see all
Images
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Lane 1 : Anti-IRF3 antibody (ab25950) at 1 µg/ml
Lane 2 : Anti-IRF3 antibody (ab25950) at 2 µg/ml
Lane 3 : Anti-IRF3 antibody (ab25950) at 4 µg/ml
All lanes : Ramos whole cell lysate.
Predicted band size: 47 kDa
Observed band size: ~44 kDa why is the actual band size different from the predicted? -
Anti-IRF3 antibody (ab25950) at 1/1000 dilution + HT-29 at 30 µg
Secondary
HRP conjugated Anti-rabbit IgG, Goat polyclonal at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDaSamples were incubated with primary antibody for 18 hours at 4ºC and blocked with 5% BSA for 1 hour at room temperature.
Protocols
Datasheets and documents
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Datasheet download
References (36)
ab25950 has been referenced in 36 publications.
- Shen H et al. Synergistically targeting synovium STING pathway for rheumatoid arthritis treatment. Bioact Mater 24:37-53 (2023). PubMed: 36582350
- Hu G et al. Mitotic SENP3 activation couples with cGAS signaling in tumor cells to stimulate anti-tumor immunity. Cell Death Dis 13:640 (2022). PubMed: 35869062
- Weerawardhana A et al. Foot-and-mouth disease virus non-structural protein 2B downregulates the RLR signaling pathway via degradation of RIG-I and MDA5. Front Immunol 13:1020262 (2022). PubMed: 36248821
- Han X et al. Knockdown of lncRNA TapSAKI alleviates LPS-induced injury in HK-2 cells through the miR-205/IRF3 pathway. Open Med (Wars) 16:581-590 (2021). PubMed: 33869780
- Jena KK et al. Autoimmunity gene IRGM suppresses cGAS-STING and RIG-I-MAVS signaling to control interferon response. EMBO Rep 21:e50051 (2020). PubMed: 32715615