Overview

  • Product name

    Anti-IRF3 (phospho S386) antibody
    See all IRF3 primary antibodies
  • Description

    Rabbit polyclonal to IRF3 (phospho S386)
  • Host species

    Rabbit
  • Specificity

    ab192796 detects endogenous levels of IRF3 only when phosphorylated at Serine 386.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human IRF3 (phospho S386) conjugated to Keyhole Limpet Haemocyanin (KLH). Peptide sequence around phosphorylation site of Serine 386(A-S-S(p)-L-E).
    Database link: Q14653

  • Positive control

    • 293 and colo cell extracts.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: 0.87% Sodium chloride, 50% Glycerol, 49% PBS

    (without Mg2+ and Ca2+)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab192796 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab192796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 47 kDa.

Target

  • Function

    Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains.
  • Tissue specificity

    Expressed constitutively in a variety of tissues.
  • Sequence similarities

    Belongs to the IRF family.
    Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
  • Post-translational
    modifications

    Constitutively phosphorylated on many serines residues. C-terminal serine/threonine cluster is phosphorylated in response of induction by IKBKE and TBK1. Ser-385 and Ser-386 may be specifically phosphorylated in response to induction. An alternate model propose that the five serine/threonine residues between 396 and 405 are phosphorylated in response to a viral infection. Phosphorylation, and subsequent activation of IRF3 is inhibited by vaccinia virus protein E3.
    Ubiquitinated; ubiquitination involves RBCK1 leading to proteasomal degradation. Polyubiquitinated; ubiquitination involves TRIM21 leading to proteasomal degradation.
    ISGylated by HERC5 resulting in sustained IRF3 activation and in the inhibition of IRF3 ubiquitination by disrupting PIN1 binding. The phosphorylation state of IRF3 does not alter ISGylation.
  • Cellular localization

    Cytoplasm. Nucleus. Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • IIAE7 antibody
    • Interferon regulatory factor 3 antibody
    • IRF 3 antibody
    • IRF-3 antibody
    • IRF3 antibody
    • IRF3_HUMAN antibody
    • MGC94729 antibody
    see all

Images

  • All lanes : Anti-IRF3 (phospho S386) antibody (ab192796) at 1/500 dilution

    Lane 1 : 293 cell extract with antigen-specific peptide
    Lane 2 : 293 cell extract
    Lane 3 : colo cell extract

    Predicted band size: 47 kDa

References

This product has been referenced in:

  • Skjesol A  et al. The TLR4 adaptor TRAM controls the phagocytosis of Gram-negative bacteria by interacting with the Rab11-family interacting protein 2. PLoS Pathog 15:e1007684 (2019). Read more (PubMed: 30883606) »
  • Li B & Zheng J MicroR-9-5p suppresses EV71 replication through targeting NF?B of the RIG-I-mediated innate immune response. FEBS Open Bio 8:1457-1470 (2018). Read more (PubMed: 30186747) »
See all 2 Publications for this product

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