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    irf3-phospho-s386-antibody-epr2346-bsa-and-azide-free-ab182859.pdf

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RecombinantRabMAb

Recombinant Anti-IRF3 (phospho S386) antibody [EPR2346] - BSA and Azide free (ab182859)

  • Datasheet
  • Certificate of Compliance
Submit a review Submit a question References (8)

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Dot Blot - Anti-IRF3 (phospho S386) antibody [EPR2346] - BSA and Azide free (ab182859)
  • Anti-IRF3 (phospho S386) antibody [EPR2346] - BSA and Azide free (ab182859)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2346] to IRF3 (phospho S386) - BSA and Azide free
  • Suitable for: WB, Dot blot
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

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Conjugation
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HRP Conjugation Kit - Lightning-Link® (ab102890)
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TCS Antibody Purification Kit (ab109207)
Protein
Recombinant Human IRF3 protein (ab205216)

View more associated products

Overview

  • Product name

    Anti-IRF3 (phospho S386) antibody [EPR2346] - BSA and Azide free
    See all IRF3 primary antibodies
  • Description

    Rabbit monoclonal [EPR2346] to IRF3 (phospho S386) - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Dot blotmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • MCF-7 cells treated with Calyculin A.
  • General notes

    ab182859 is the carrier-free version of ab76493.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.20
    Constituent: 100% PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2346
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interferons
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Immunology
    • Innate Immunity
    • TLR Signaling

Associated products

  • Alternative Versions

    • Anti-IRF3 (phospho S386) antibody [EPR2346] (ab76493)
  • Assay kits

    • IRF3 Transcription Factor Assay Kit (Colorimetric) (ab207210)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human IRF3 protein (ab205216)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab182859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.
Dot blot
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.
Dot blot
Use at an assay dependent concentration.

Target

  • Function

    Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains.
  • Tissue specificity

    Expressed constitutively in a variety of tissues.
  • Sequence similarities

    Belongs to the IRF family.
    Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
  • Post-translational
    modifications

    Constitutively phosphorylated on many serines residues. C-terminal serine/threonine cluster is phosphorylated in response of induction by IKBKE and TBK1. Ser-385 and Ser-386 may be specifically phosphorylated in response to induction. An alternate model propose that the five serine/threonine residues between 396 and 405 are phosphorylated in response to a viral infection. Phosphorylation, and subsequent activation of IRF3 is inhibited by vaccinia virus protein E3.
    Ubiquitinated; ubiquitination involves RBCK1 leading to proteasomal degradation. Polyubiquitinated; ubiquitination involves TRIM21 leading to proteasomal degradation.
    ISGylated by HERC5 resulting in sustained IRF3 activation and in the inhibition of IRF3 ubiquitination by disrupting PIN1 binding. The phosphorylation state of IRF3 does not alter ISGylation.
  • Cellular localization

    Cytoplasm. Nucleus. Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm.
  • Target information above from: UniProt accession Q14653 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3661 Human
    • Omim: 603734 Human
    • SwissProt: Q14653 Human
    • Unigene: 289052 Human
    • Unigene: 75254 Human
    • Alternative names

      • IIAE7 antibody
      • Interferon regulatory factor 3 antibody
      • IRF 3 antibody
      • IRF-3 antibody
      • IRF3 antibody
      • IRF3_HUMAN antibody
      • MGC94729 antibody
      see all

    Images

    • Dot Blot - Anti-IRF3 (phospho S386) antibody [EPR2346] - BSA and Azide free (ab182859)
      Dot Blot - Anti-IRF3 (phospho S386) antibody [EPR2346] - BSA and Azide free (ab182859)

      This Dot Blot data was generated using the same anti-phospho IRF3 S386 antibody clone, EPR2346, in a different buffer formulation (cat# ab76493).

      Dot blot analysis of IRF3 single phospho peptide pS386 (lane 1) and IRF3 non-phospho peptide (lane 2) with ab76493 at 1/1000. Blocking and diluting buffer was 5% NFDM/TBST. The secondary antibody used was ab97051 Peroxidase conjugated Goat Anti-Rabbit IgG, (H+L) at 1/100,000.

    • Anti-IRF3 (phospho S386) antibody [EPR2346] - BSA and Azide free (ab182859)
      Anti-IRF3 (phospho S386) antibody [EPR2346] - BSA and Azide free (ab182859)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (8)

    Publishing research using ab182859? Please let us know so that we can cite the reference in this datasheet.

    ab182859 has been referenced in 8 publications.

    • Lu HL & Liao F Melanoma differentiation-associated gene 5 senses hepatitis B virus and activates innate immune signaling to suppress virus replication. J Immunol 191:3264-76 (2013). PubMed: 23926323
    • Charoenthongtrakul S  et al. RING finger protein 11 targets TBK1/IKKi kinases to inhibit antiviral signaling. PLoS One 8:e53717 (2013). WB . PubMed: 23308279
    • Herman M  et al. Heterozygous TBK1 mutations impair TLR3 immunity and underlie herpes simplex encephalitis of childhood. J Exp Med 209:1567-82 (2012). PubMed: 22851595
    • Ma X  et al. Molecular basis of Tank-binding kinase 1 activation by transautophosphorylation. Proc Natl Acad Sci U S A 109:9378-83 (2012). WB . PubMed: 22619329
    • Alam S  et al. Galectin-9 expression in endothelial cells is positively regulated by histone deacetylase 3. J Biol Chem : (2011). WB ; Human . PubMed: 22027828
    • Gao L  et al. ABIN1 Protein Cooperates with TAX1BP1 and A20 Proteins to Inhibit Antiviral Signaling. J Biol Chem 286:36592-602 (2011). WB . PubMed: 21885437
    • Liu F  et al. Klotho suppresses RIG-I-mediated senescence-associated inflammation. Nat Cell Biol 13:254-62 (2011). PubMed: 21336305
    • Liu XY  et al. Tom70 mediates activation of interferon regulatory factor 3 on mitochondria. Cell Res 20:994-1011 (2010). WB ; Human . PubMed: 20628368

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