Recombinant Anti-IRF3 (phospho S386) antibody [EPR2346] - BSA and Azide free (ab182859)
![Dot Blot - Anti-IRF3 (phospho S386) antibody [EPR2346] - BSA and Azide free (ab182859)](https://www.abcam.com/ps/products/182/ab182859/Images/ab182859-295829-anti-irf3-phospho-s386-antibody-epr2346-dot-blot.jpg "Dot Blot - Anti-IRF3 (phospho S386) antibody [EPR2346] - BSA and Azide free (ab182859)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2346] to IRF3 (phospho S386) - BSA and Azide free
- Suitable for: WB, Dot blot
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-IRF3 (phospho S386) antibody [EPR2346] - BSA and Azide free
See all IRF3 primary antibodies -
Description
Rabbit monoclonal [EPR2346] to IRF3 (phospho S386) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, Dot blotmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- MCF-7 cells treated with Calyculin A.
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General notes
ab182859 is the carrier-free version of ab76493.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2346 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab182859 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.
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| Dot blot |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 47 kDa. |
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Dot blot
Use at an assay dependent concentration. |
Target
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Function
Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains. -
Tissue specificity
Expressed constitutively in a variety of tissues. -
Sequence similarities
Belongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain. -
Post-translational
modificationsConstitutively phosphorylated on many serines residues. C-terminal serine/threonine cluster is phosphorylated in response of induction by IKBKE and TBK1. Ser-385 and Ser-386 may be specifically phosphorylated in response to induction. An alternate model propose that the five serine/threonine residues between 396 and 405 are phosphorylated in response to a viral infection. Phosphorylation, and subsequent activation of IRF3 is inhibited by vaccinia virus protein E3.
Ubiquitinated; ubiquitination involves RBCK1 leading to proteasomal degradation. Polyubiquitinated; ubiquitination involves TRIM21 leading to proteasomal degradation.
ISGylated by HERC5 resulting in sustained IRF3 activation and in the inhibition of IRF3 ubiquitination by disrupting PIN1 binding. The phosphorylation state of IRF3 does not alter ISGylation. -
Cellular localization
Cytoplasm. Nucleus. Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 3661 Human
- Omim: 603734 Human
- SwissProt: Q14653 Human
- Unigene: 289052 Human
- Unigene: 75254 Human
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Alternative names
- IIAE7 antibody
- Interferon regulatory factor 3 antibody
- IRF 3 antibody
see all
Images
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Dot Blot - Anti-IRF3 (phospho S386) antibody [EPR2346] - BSA and Azide free (ab182859)
This Dot Blot data was generated using the same anti-phospho IRF3 S386 antibody clone, EPR2346, in a different buffer formulation (cat# ab76493).
Dot blot analysis of IRF3 single phospho peptide pS386 (lane 1) and IRF3 non-phospho peptide (lane 2) with ab76493 at 1/1000. Blocking and diluting buffer was 5% NFDM/TBST. The secondary antibody used was ab97051 Peroxidase conjugated Goat Anti-Rabbit IgG, (H+L) at 1/100,000.
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![Anti-IRF3 (phospho S386) antibody [EPR2346] - BSA and Azide free (ab182859)](https://www.abcam.com/ps/products/182/ab182859/Images/ab182859-2-benefits-of-recombinant-antibodies.png)
Anti-IRF3 (phospho S386) antibody [EPR2346] - BSA and Azide free (ab182859)
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (8)
ab182859 has been referenced in 8 publications.
- Lu HL & Liao F Melanoma differentiation-associated gene 5 senses hepatitis B virus and activates innate immune signaling to suppress virus replication. J Immunol 191:3264-76 (2013). PubMed: 23926323
- Charoenthongtrakul S et al. RING finger protein 11 targets TBK1/IKKi kinases to inhibit antiviral signaling. PLoS One 8:e53717 (2013). WB . PubMed: 23308279
- Herman M et al. Heterozygous TBK1 mutations impair TLR3 immunity and underlie herpes simplex encephalitis of childhood. J Exp Med 209:1567-82 (2012). PubMed: 22851595
- Ma X et al. Molecular basis of Tank-binding kinase 1 activation by transautophosphorylation. Proc Natl Acad Sci U S A 109:9378-83 (2012). WB . PubMed: 22619329
- Alam S et al. Galectin-9 expression in endothelial cells is positively regulated by histone deacetylase 3. J Biol Chem : (2011). WB ; Human . PubMed: 22027828
- Gao L et al. ABIN1 Protein Cooperates with TAX1BP1 and A20 Proteins to Inhibit Antiviral Signaling. J Biol Chem 286:36592-602 (2011). WB . PubMed: 21885437
- Liu F et al. Klotho suppresses RIG-I-mediated senescence-associated inflammation. Nat Cell Biol 13:254-62 (2011). PubMed: 21336305
- Liu XY et al. Tom70 mediates activation of interferon regulatory factor 3 on mitochondria. Cell Res 20:994-1011 (2010). WB ; Human . PubMed: 20628368
