Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IRF5 antibody

Average
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Macrophages)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Ventana Ultra CC1
Permeabilization
No
Specification
Macrophages
Blocking step
Hydrogen Peroxide as blocking agent for 4 minute(s) · Concentration: 3.0% · Temperature: 36°C
Fixative
Formaldehyde

Other product details

Incubation time
32 minute(s) · Temperature: 37°C · Diluent: A. Menarini Background Reducing Diluent
Dilution
1/1000

Secondary antibody

Concentration
40 µg/ml
Name
Non-Abcam antibody was used: OptiView HRP Multimer
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

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Submitted Jan 25 2016

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