Overview

  • Product name

  • Description

    Rabbit polyclonal to IRF5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide within Mouse IRF5 aa 175-225. The exact sequence is proprietary.
    Database link: P56477

  • Positive control

    • WB: Mouse bone marrow dendritic cell lysate. IP: Mouse bone marrow dendritic cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab245475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/200 - 1/10000. Predicted molecular weight: 56 kDa.

Target

  • Involvement in disease

    Genetic variations in IRF5 are associated with susceptibility to inflammatory bowel disease type 14 (IBD14) [MIM:612245]. IBD14 is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
    Genetic variations in IRF5 are associated with susceptibility to systemic lupus erythematosus type 10 (SLEB10) [MIM:612251]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
    Genetic variations in IRF5 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures.
  • Sequence similarities

    Belongs to the IRF family.
    Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Interferon regulatory factor 5 antibody
    • Interferon regulatory factor 5 bone marrow variant antibody
    • IRF 5 antibody
    • IRF-5 antibody
    • Irf5 antibody
    • IRF5_HUMAN antibody
    • SLEB10 antibody
    see all

Images

  • IRF5 was immunoprecipitated from 1 mg (20% of IP loaded) mouse bone marrow dendritic cell lysate using ab245475 at 6 µg/mg lysate. Western blot was performed on the immunoprecipitate using ab245475 at 1 µg/ml.

    Lane 1: ab245475 IP in mouse bone marrow dendritic cell lysate.
    Lane 2: Control IgG in mouse bone marrow dendritic cell lysate.

    Detection: Chemiluminescence with an exposure time of 30 seconds.

     

  • All lanes : Anti-IRF5 antibody (ab245475) at 0.04 µg/ml

    Lane 1 : Mouse bone marrow dendritic cell lysate at 50 µg
    Lane 2 : Mouse bone marrow dendritic cell lysate at 15 µg

    Developed using the ECL technique.

    Predicted band size: 56 kDa


    Exposure time: 3 minutes

References

ab245475 has not yet been referenced specifically in any publications.

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