Product nameAnti-IRF5 antibody - ChIP Grade
See all IRF5 primary antibodies
DescriptionRabbit polyclonal to IRF5 - ChIP Grade
Tested applicationsSuitable for: CHIPseq, WBmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow
- This antibody gave a positive signal in the following lysates: HeLa (Human epithelial carcinoma cell line) whole cell Liver (Mouse) Tissue Kidney (Mouse) Tissue Liver (Rat) Tissue
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
- Epigenetics and Nuclear Signaling
- Domain Families
- HLH / Leucine Zipper
ChIP Related Products
Our Abpromise guarantee covers the use of ab21689 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|CHIPseq||Use at an assay dependent concentration. PubMed: 25159141|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 58 kDa).|
Involvement in diseaseGenetic variations in IRF5 are associated with susceptibility to inflammatory bowel disease type 14 (IBD14) [MIM:612245]. IBD14 is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
Genetic variations in IRF5 are associated with susceptibility to systemic lupus erythematosus type 10 (SLEB10) [MIM:612251]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
Genetic variations in IRF5 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures.
Sequence similaritiesBelongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
- Information by UniProt
- Interferon regulatory factor 5 antibody
- Interferon regulatory factor 5 bone marrow variant antibody
- IRF 5 antibody
GM-BMDMs from RelAFl/Fl mice were infected with either Cre (black bars) or Empty (white bars) adenovirus and stimulated with LPS (100ng/ml) for 2 hr or left unstimulated and used in ChIP analysis for IRF5 recruitment on Il-6 and Il-1a loci. Nuclear lysates were immunoprecipitated with 10µg of ab21689. Data indicate mean percent input relative to genomic DNA ± SD of a representative experiment.
All lanes : Anti-IRF5 antibody - ChIP Grade (ab21689) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate - normal tissue
Lane 2 : Kidney (Mouse) Tissue Lysate
Lane 3 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Manni M et al. Regulation of age-associated B cells by IRF5 in systemic autoimmunity. Nat Immunol 19:407-419 (2018). Read more (PubMed: 29483597) »
- Seneviratne AN et al. Interferon Regulatory Factor 5 Controls Necrotic Core Formation in Atherosclerotic Lesions by Impairing Efferocytosis. Circulation 136:1140-1154 (2017). Read more (PubMed: 28698173) »