Product nameAnti-IRF5 antibody - ChIP Grade
See all IRF5 primary antibodies
DescriptionGoat polyclonal to IRF5 - ChIP Grade
SpecificityThis antibody is expected to recognize all four reported isoforms (NP_002191.1; NP_116032.1; NP_001092097.1; NP_001092099.1).
Tested applicationsSuitable for: IHC-P, ChIP, WB, IPmore details
Species reactivityReacts with: Dog, Human
Predicted to work with: Mouse, Rat, Cow
- Purchase matching WB positive control:Recombinant Human IRF5 protein
- A549 lysate Human cervix. HEK293
Principal Names – IRF5; interferon regulatory factor 5 Official Gene Symbol - IRF5 GenBank Accession Number – NP_002191; NP_116032 LocusLink ID - 3663 (human) Gene Ontology terms - RNA polymerase III transcription factor; cell growth and/or maintenance
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Epigenetics and Nuclear Signaling
- Domain Families
- HLH / Leucine Zipper
ChIP Related Products
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab2932 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ChIP||Use at an assay dependent dilution. PubMed: 19028697|
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).Can be blocked with Human IRF5 peptide (ab22939).|
|IP||Use at an assay dependent dilution. PubMed: 19028697|
Involvement in diseaseGenetic variations in IRF5 are associated with susceptibility to inflammatory bowel disease type 14 (IBD14) [MIM:612245]. IBD14 is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
Genetic variations in IRF5 are associated with susceptibility to systemic lupus erythematosus type 10 (SLEB10) [MIM:612251]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
Genetic variations in IRF5 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures.
Sequence similaritiesBelongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
- Information by UniProt
- Interferon regulatory factor 5 antibody
- Interferon regulatory factor 5 bone marrow variant antibody
- IRF 5 antibody
p53± cells were treated over a time course. The cells were subjected to IP and ChIP analysis using the indicated antibodies and primers. Input is shown in the top four panels; PCR results are shown in the bottom panels. A single panel is shown as representative of amplification from all primer sets after IP with IgG control antibodies for IRF-5.
ab2932 staining (2
µg/ml) of A549 lysate (RIPA buffer, 30 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. ab2932 staining (2µg/ml) of A549 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
HEK293 (ab7902) overexpressing human IRF5 and probed with ab2932 at 1µg/ml (mock transfection in lane 2).
IHC image of ab2932 staining in human normal cervix (ab29226) formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2932, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This product has been referenced in:
- Li D et al. Specific detection of interferon regulatory factor 5 (IRF5): A case of antibody inequality. Sci Rep 6:31002 (2016). Read more (PubMed: 27481535) »
- Massimino M et al. IRF5 is a target of BCR-ABL kinase activity and reduces CML cell proliferation. Carcinogenesis 35:1132-43 (2014). Human . Read more (PubMed: 24445143) »