Western blot abreview for Anti-IRF5 antibody - ChIP Grade

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (monocyte-derived dendritic cells)
Loading amount
30 µg
Specification
monocyte-derived dendritic cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/100
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk

Secondary antibody

Name
Non-Abcam antibody was used: Anti-Goat IgG (H&L) (donkey) antibody IRdye 800
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW

Detection

Detection method
LICOR Odyssey
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 65 kDa

Additional data

Additional Notes
Western blot represents 30 microgram lysate from primary human monocyte-derived dendritic cells.

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Submitted Jan 17 2013

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